Recombinant murine IL-5 enhances IgA production by rat splenic B cells

McGhee, D W; Beagley, K W; McGhee, J R

doi:10.1007/978-94-009-1848-1_34

D W McGhee⁶,
K W Beagley⁶ &
J R McGhee⁶

19 Accesses

Abstract

Recent reports using highly purified or recombinant interleukin 5 (IL-5) have determined that this interleukin induces enhanced IgA synthesis by lipopolysaccharide (LPS) triggered murine splenic B cells as well as unstimulated Peyer’s patch B cells (1–3). The recombinant form of IL-5 is not yet available for the rat, however, a previous report by our group (4) has indicated that interleukin-containing culture supernatants from murine EL-4 and B151 K12 cell lines could support plaque forming cell responses by rat B cell cultures. Because of the importance of the rat models in mucosal immunity, we chose to study the effect of recombinant murine IL-5 (rmIL-5) on the polyclonal antibody response of rat spleen B cells.

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Authors and Affiliations

Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA
D W McGhee, K W Beagley & J R McGhee

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D W McGhee
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K W Beagley
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J R McGhee
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Editors and Affiliations

Department of Paediatric Gastroenterology, St Bartholomews Hospital, London, UK
Thomas T MacDonald PhD MRCPath (Wellcome Senior Lecturer) (Wellcome Senior Lecturer)
Department of Oral Medicine and Pathology, Guy’s Hospital, London, UK
Stephen J Challacombe PhD BDS MRCPath (Professor) (Professor)
Department of Veterinary Medicine, The University, Bristol, UK
Paul W Bland PhD (Lecturer) & Christopher R Stokes PhD (Senior Research Fellow) (Lecturer) & (Senior Research Fellow)
Department of Medicine, St James’s University Hospital, London, UK
Richard V Heatley MD MRCP (Senior Lecturer) (Senior Lecturer)
Department of Bacteriology and Immunology, The University, Glasgow, UK
Allan Mcl Mowat PhD MB ChB (Lecturer) (Lecturer)

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McGhee, D.W., Beagley, K.W., McGhee, J.R. (1990). Recombinant murine IL-5 enhances IgA production by rat splenic B cells. In: MacDonald, T.T., Challacombe, S.J., Bland, P.W., Stokes, C.R., Heatley, R.V., Mowat, A.M. (eds) Advances in Mucosal Immunology. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1848-1_34

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DOI: https://doi.org/10.1007/978-94-009-1848-1_34
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7323-3
Online ISBN: 978-94-009-1848-1
eBook Packages: Springer Book Archive

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