Abstract
Recently molecular technology has enabled reporter and marker genes to be integrated in the fungal genome. The resulting transformants can then be monitored after introduction into natural environments. This paper summarizes the suitability of using a GUS transformant for monitoring the presence, the population development, and the activity of a specific strain of T. harzianum deliberately released into the environment.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abd-El Moity T.H., Papavizas, G.C., and Shatla, M.N. (1982) Introduction of new isolates of Trichoderma harzianum tolerant to fungicides and their experimental use for control of white rot of onion. Phytopathology 72:396–400.
Ahmad, J.S., and Baker, R. (1987) Competetive saprophytic ability and cellulytic activity of rhizosphere competent mutants of Trichoderma harzianum. Phytopathology 77:358–362.
Brock, T.D. (1987) The study of microorganisms in situ: progress and problems. Pages 1–17 in: Ecology of Microbial Communities. M. Fletcher, T.R.G. Gray and J.G. Jones, eds. Symp. Soc. Gen. Microbiol. Vol. 41.
Chet, I. (1987) Trichoderma-application, mode of action, and potential as a biocontrol agent of soilborne plant pathogenic fungi. Pages 137–160 in: Innovative Approaches to Plant Disease Control. I. Chet, ed. Wiley, New York.
Couteaudier, Y., Daboussi, M.-J., Eparvier, A., Langin, T., and Orcival, J. (1993) The GUS gene fusion system (Escherichia coli ß-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum. Appl. Environ. Microbiol. 59:1767–1773.
Davet, P. 1979. Technique pour l’analyse des populations de Trichoderma et de Gliocladium virens dans le sol. Ann. Phytopathol. 11:529–533.
Elad, Y., Chet, I., and Henis, Y. (1981) A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica 9:59–67.
Eparvier, A., and Alabouvette, C. (1994) Competition between pathogenic and non pathogenic Fusarium oxysporum for root colonization. Biocontrol Sci. Technol. 4:35–47.
Gallagher, S.R. (1992) GUS protocols: Using the GUS gene as a reporter of gene expression. Academic Press, San Diego, 221 pp.
Green, H., and Jensen, D.F. (1995) A tool for monitoring Trichoderma harzianum: II. The use of a GUS transformant for ecological studies in the rhizosphere. Phytopathology, 85:1436–1440.
Jefferson, R. A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5:387–405.
Jensen, D.F., and Wolffhechel, H. (1995) The use of fungi, particularly Trichoderma spp. and Gliocladium spp., to control root rot and damping-off diseases. Pages 177–189 in: Biocontrol Agents: Benefits and Risks. H. Hokkanen, and J.M. Lynch eds. Cambridge University Press, Cambridge.
Liljeroth, E., Jansson, H.-B., and Schäfer, W. (1994) Transformation of Bipolaris sorokiniana with the GUS-gene and use for studying fungal colonization of barley roots. Phytopathology 84:1484–89.
Oliver, R.P., Farman, M.L., Jones, J.D.G., and Hammond-Kosack, K.E. (1993) Use of fungal transformants expressing ß-glucuronidase activity to detect infection and measure hyphal biomass in infected plant tissues. Mol. Plant-Microbe Interact. 6:521–525.
Papavizas, G.C. (1985) Trichoderma and Gliocladium: biology, ecology, and potential for biocontrol. Annu. Rev. Phytopathol. 23:23–54.
Papavizas, G.C, Lewis, J.A., and Abd-El Moity, T.H. (1982) Evaluation of new biotypes of Trichoderma harzianum for tolerance to benomyl and enhanced biocontrol capabilities. Phytopathology 72:126–132.
Papavizas, G.C., and Lumsden, R.D. (1982) Improved medium for isolation of Trichoderma spp. from soil. Plant Dis. 66:1019–1020.
Parkinson, D., and Coleman, D.C. (1991) Methods for assessing soil microbial populations, activity and biomass. Agric. Ecosyst. Environ. 34:3–33.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning. A laboratory manual. Second edition. Cold Spring Harbour Laboratory Press, Cold Spring Harbour.
Söderström, B.E. (1977) Vital staining of tungi in pure cultures and in soil with fluorescein diacetate. Soil Biol. Biochem. 9:59–63.
Thrane, C, Lübeck, M., Green, H., Defégu, Y., Allerup, S., Thrane, U., and Jensen, D.F. (1995) A tool for monitoring Trichoderma harzianum: I. Transformation with the GUS gene by protoplast technology. Phytopathology, 85:1428–1435.
Torres, M., Viladrich, R., Sanchis, V., and Canela, R. (1992) Influenxe of age on ergosterol content in mycelium of Aspergillus ochraceus. Lett. Appl. Microbiol. 15:20–22.
Warcup, J.H. (1955) On the origin of colonies of fungi developing on soil dilution plates. Trans. Br. Mycol. Soc. 38:298–262.
Wilson, K.J., Giller, K.E., and Jefferson, R.A. (1991) ß-glucuronidase (GUS) operon fusion as a tool for studying plant-microbe interactions. Pages 226–229 in: Advances in Molecular Genetics of Plant-Microbe Interactions. Vol. 1. H. Hennecke, and D.P.S. Verma, eds. Kluwer Academic Publishers, the Netherlands.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Kluwer Academic Publishers
About this chapter
Cite this chapter
Green, H., Jensen, D.F. (1996). Monitoring of a Gus Transformed Strain of Trichoderma Harzianum in Soil and Rhizosphere. In: Jensen, D.F., Jansson, HB., Tronsmo, A. (eds) Monitoring Antagonistic Fungi Deliberately Released into the Environment. Developments in Plant Pathology, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1698-2_11
Download citation
DOI: https://doi.org/10.1007/978-94-009-1698-2_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7260-1
Online ISBN: 978-94-009-1698-2
eBook Packages: Springer Book Archive