Abstract
The filamentous marine brown algae Ectocarpus siliculosus and Feldmannia simplex are infected by host specific DNA-viruses. Under Percoll isolation, Ectocarpus siliculosus-virus (EsV)-particles maintained their infective potential.
The EsV has a circular genome of dsDNA with a size of 320 kb. A restriction map has been established. The gene of a major coat protein (gpl) was detected in a genomic library and partly sequenced. Using gpl- sequences for polymerase chain reaction (PCR) amplification analysis, EsV-specific sequences could be detected in various symptom-free, clonal cultures of Ectocarpus. The PCR was also used to follow the passage of the virus genome during the meiosis of hosts. A monospecific antibody against recombinant gpl was used for immunostaining and infection experiments.
The Feldmannia simplex-virus (FlexV-1) has a circular genome with a size of 220 kb and a 43% G+C content. FsV-DNA contains methylated bases. 5-methylcytosine (5 mC) makes up 12% of the total cytosines.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bräutigam, M., M. Klein, R. Knippers & D.G. Müller, 1995. Inheritance and meiotic elimination of a virus genome in the host Ectocarpus siliculosus (Phaeophyceae). J. Phycol. 31: 823–827.
Flateau, E., F. A. Gonzales, F. A. Michalowski & P. A. Jones, 1984. DNA-methylation in 5-aza-2-deoxycytidine-resistant variants of C3H 10T1/2 C18 cells. Mol. cell. Biol.: 4: 2098–2102.
Frieß-Klebl, A.-K., R. Knippers &D. G. Müller, 1994. Isolation and characterization of a DNA virus infecting Feldmannia simplex (Phaeophyceae). J. Phycol. 30: 653–658.
Gershoni, J. M., E. A. Bayer & M. Wilchek, 1985. Blot analyses of glycoconjugates: enzyme hydrazide, a novel reagent for the detection of aldehydes. Analyt. Biochem. 146: 59–63.
Goff L. J. & D. Moon, 1993. PCR amplification of nuclear and plastid genes from algal herbarium specimens and algal spores. J. Phycol. 29: 381–384.
Henry, E. C. & R. H. Meints, 1992. A persistent virus infection in Feldmannia (Phaeophyceae). J. Phycol. 28: 517–526.
Klein, M., S. T. J. Lanka, R. Knippers & D. G. Müller, 1995. Coat protein of the Ectocarpus siliculosus virus. Virology 206: 520–526.
Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Lanka, S., M. Klein, U. Ramsperger, D. G. Müller & R. Knippers, 1993. Genome structure of a virus infecting the marine brown alga Ectocarpus siliculosus. Virology 193: 802–811.
Matagne, A., B. Joris & J. M. Frere, 1991. Anomalous behaviour of a protein during SDS/PAGE corrected by chemical modification of carboxyl groups. Biochem. J. 280: 553–556.
Meshbah, M., U. Premachandran & W. B. Whitman, 1989. Precise measurement of the G+C content of desoxyribonucleic acid by high performance liquid chromatography. Int. J. syst. Bacteriol. 39:159–167.
Müller, D. G., 1991a. Marine virioplankton produced by infected Ectocarpus siliculosus (Phaeophyceae). Mar. Ecol. Prog. Ser. 76: 101–102.
Müller, D. G., 1991b. Mendelian segregation of a virus genome during host meiosis in the brown alga Ectocarpus siliculosus. J. Plant Physiol. 137: 739–743.
Müller, D. G. & E. R. Parodi, 1993. Transfer of a marine DNA virus from Ectocarpus to Feldmannia (Ectocarpales, Phaeophyceae): aberrant symptoms and restitution of the host. Protoplasma 175: 121–125.
Müller, D. G. & B. Stäche, 1992. Worldwide occurence of virusinfections in filamentous marine brown algae. Helgoländer Meeresunters. 46: 1–8.
Müller, D. G., H. Kawai, B. Stäche & S. Lanka, 1990. A virus infection in the marine brown alga Ectocarpus siliculosus (Phaeophyceae). Bot. Acta 103: 72–82.
Towbin, H., T. Staehelin & J. Gordon, 1979. Electrophoretic transfer of proteins of Polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. nat. Acad. Sci. U.S.A. 67: 4350–4354.
Van Etten, J. L., L. C. Lane & R. H. Meints, 1991. Viruses and viruslike particles of eukaryotic algae. Microbiol. Rev. 55: 586–620.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Kluwer Academic Publishers
About this paper
Cite this paper
Klebl, AK., Bräutigam, M., Klein, M., Kapp, M., Knippers, R., Müller, D.G. (1996). Molecular and biochemical studies of virus infections in two filamentous brown algae. In: Lindstrom, S.C., Chapman, D.J. (eds) Fifteenth International Seaweed Symposium. Developments in Hydrobiology, vol 116. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1659-3_14
Download citation
DOI: https://doi.org/10.1007/978-94-009-1659-3_14
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7242-7
Online ISBN: 978-94-009-1659-3
eBook Packages: Springer Book Archive