Abstract
A method is described by which the integument of Brachionus plicatilis, together with its intracellular lamina, is quickly dissolved before other parts or tissues of the animal are destroyed. After removing the integument several parts of the body can be separated and fractionated in a more or less intact state by centrifugation in a Percoll gradient. The measurement of enzyme activities has indicated that this procedure might provide a way of localizing enzymes within the rotifer body.
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References
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© 1995 Kluwer Academic Publishers
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Kleinow, W., Röhrig, A. (1995). Enzyme activity measurements on isolated organs of Brachionus plicatilis (Rotifera). In: Ejsmont-Karabin, J., Pontin, R.M. (eds) Rotifera VII. Developments in Hydrobiology, vol 109. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1583-1_23
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DOI: https://doi.org/10.1007/978-94-009-1583-1_23
Publisher Name: Springer, Dordrecht
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Online ISBN: 978-94-009-1583-1
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