Summary
The study of the molecular location of acid and alkaline phosphatases in a compost of urban refuse was initiated after their extraction using 0,05 M pyrophosphate at pH 9 and in the presence of 1 M KCl to modify its ionic strength. The extract was divided by ultrafiltration in cell-bound (UF3 fraction with ϕ>0,45 μm) and exocellular enzymes: UF23 with ϕ<0,45 μm and mol. wt.> 100 kD; UF22 with 100<moi. wt.<30 kD; UF21, with 30 <mol. wt.>l0 kD and UF1, with mol. wt.<l0 kD. Thereafter UF3 was divided by sonication in their intracellular (Ei) and membranebound components (Em) and after solubilization with Triton X-100 in enzymes located outside (Emp) and inside the membrane (Ems)
The highest activity of acid phosphatase appeared in the UF3 fraction, its extraction being unaffected by the introduction of KCl in the extractant. The UF3 fractionation located the acid PA in the membrane-protein fractions (Em). Alkaline phosphatase was predominant in the fraction UF23 showing 86% of the activity displayed by all the fractions; the increase in the ionic strength reduced this activity in UF23 (-68%) increasing the activity in UF22 (5,2% to 24,2%). In UF3, the alkaline phosphatase had an intracellular localization (Ei fraction) showing 90% of the activity displayed by UF3
Different mechanisms of stabilization for compost enzymes were postulated: acid phosphatases may be associated to membranous components of living or dead microbial cells with low solubility in the pyrophosphate extractant and alkaline phosphatase may form a soluble complex enzyme-organic matter of high mol. wt. dissociable at high ionic strength.
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References
Boyd, S. A. and Mortland, M. M. (1990). Enzyme interactions with clays and clay-organic matter complexes. In Soil Biochemistry, (J. M. Bollag and G. Stotzky. Eds), pp. 1–28. Marcel Dekker, New York.
Bretaudiere, J. P. and Spillman, T. (1984). Alkaline phosphatases. In Methods of Enzymatic Analysis. (H. U. Bergmeyer, J. Bergmeyer and M. Graßl, Eds), pp. 75–106. Verlag Chern ie, Weinheim (Gennany).
Bums, R. G. (1986). Interactions of Enzymes with Soil Mineral and Organic Colloids. In Interactions of Soil Minerals with Natural Organics and Microbes. (P. M. Huang and M. Schnitzer. Eds). pp. 429–451. Soil Science of America. Madison, Wis. (USA)
De Bertoldi, M. (1992). The control of the composting process and quality of en products. In Composting and Compost Quality Assurance Criteria. (D. V. Jackson. J. M. Merillot and P.L’Hennite. Eds), pp. 85–93. Commission of the European Communities. Brussels (Belgium).
Fasman, G. D. (1989). Practical Handbook of Biochemistry and Molecular Biology. p. 164 CRC Press. Florida (USA).
García, C., Hernández, T., Costa, F., Ceccanti, B. and Masciandaro, G. (1993). Kinetics of phosphatase activity in organic wastes. Soil Biology and Biochemistry, 25. 561–565.
He, X. T., Traina, S. J. and Logan, T. J. (1992). Chemical properties of Municipal Solid Waste composts. Journal of Environmental Quality, 21,318–329.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biology and Biochemistry, 193.265–275.
Martens, D. A. and Frankenberger, W. T. J. (1991). Saccharide composition of extracellular polymers produced by soil microorganisms. Soil Biology and Biochemistry. 23, 731–736.
Moss, D. W. (1984). Acid phosphatases. In Methods of Enzymatic Analysis. (H. U. Bergmeyer, J. Bergmeyer and M. Graßl. Eds). pp. 92–105. Verlag Chemie. Weinheim (Gennany).
Nakas, J. P., Gould, W. D. and Klein, D. A. (1987). Origin and expression of phosphatase activity in a grassland soil. Soil Biology and Biochemistry, 19. 13–18.
Nannipieri, P., Ceccanti, B. and Bianchi, D. (1988). Characterization of humus-phosphatase complexes extracted to soil. Soil Biology and Biochemistry, 20. 683–691.
Nannipieri, P., Ceccanti, B., Cervelli, S. and Matarese, E. (1980). Extraction of phosphatase. urease. proteases, organic carbon and nitrogen from soil. Soil Science Society of America Journal. 44. 1011–1016.
Rad, J. C. (1992). Materia Orgánica Residual Urbana: Extracción y caracterización de actividades enzimáticas de interés agrotecnolágico. Ph D Thesis. University of Valladolid (Spain).
Rojo, M. J., González-Carcedo, S. and Pérez-Mateos, M. (1990). Distribution and characterization of phosphatase and organic phosphorus in soil fractions. Soil Biology and Biochemistry. 22, 169–174.
Ruggiero, P. and Radogna, V. M. (1988). Humic acid-tyrosinase interactions as a model of soil humic enzyme complexes. Soil Biology and Biochemistry, 20, 353–359.
Tabatabai, M. A. and Bremner, J. M. (1969). Use of p-nitrophenol phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1,301–307.
Van Belle, W. A. (1992). The role of composting in an integrated waste management system. In Composting and Compost Quality Assurance Criteria. (D. Y. Jackson, J. M. Merillot and P. L’Hermite, Eds). pp. 5–23. Commision of the European Communities, Brussels (Belgium).
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Rad Moradillo, J.C., González Carcedo, S. (1996). Different Location of Acid and Alkaline Phosphatases Extracted From a Compost of Urban Refuse. In: de Bertoldi, M., Sequi, P., Lemmes, B., Papi, T. (eds) The Science of Composting. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1569-5_28
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DOI: https://doi.org/10.1007/978-94-009-1569-5_28
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