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Detection

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Book cover Handbook of Capillary Electrophoresis Applications

Abstract

Although analysis in capillary electrophoresis has the advantage of speed with high separation efficiency, its concentration sensitivity is unfavorably low. Since the capillary diameter in CE is less than 100µm and the entire volume of the capillary in most cases is less than 10 µl, the optical path should be short enough to detect analyte zones without loss of separation efficiency. Consequently, the detection limit is between 1 and 0.1 ppm with conventional on-line UV detection. Several studies have been carried out to enhance the detection sensitivity, e.g. sample preconcentration, choice of detection system including strong light source, a detector with high sensitivity and efficient optical alignment, and derivatization of analytes [1].

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Authors
  1. K. Kitagishi
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Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Kitagishi, K. (1997). Detection. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_7

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

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