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Capillary electrophoresis of metal complexes

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Handbook of Capillary Electrophoresis Applications

Abstract

Currently, capillary zone electrophoresis (CZE) is attracting considerable interest as an alternative technique for the determination of metal ions. However, a range of metal ions such as transition and lanthanide metal ions exhibit nearly identical charge and size, so that a separation solely based on differences in the electrophoretic mobilities presents a serious problem. The most successful approach to optimization of separation selectivity for metal ions is the employment of complex-forming reactions that can take place in the capillary during the separation or in the sample before injection. The mobility of metal cations can be manipulated by addition of weak complexing agents such as organic acids to the carrier electrolyte. The higher the respective complex-formation constant, the lower will be the electrophoretic mobility due to the decreased effective charge (the effective charge is still positive, as the complexing properties of organic acids are not very strong). This strategy and various applications have been discussed in Chapter 36 on Inorganic Ions. Another possibility for improving the separation selectivity is the use of strong complexing reagents that allow the full conversion of metal ions into positively charged, negatively charged or neutral complexes. In this case, the complexation reaction can be carried out in the sample before injection (precapillary complexation), if the thermodynamic and kinetic stability of the resulting complexes is high enough.

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Authors
  1. W. Buchberger
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Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Buchberger, W. (1997). Capillary electrophoresis of metal complexes. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_35

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_35

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

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