Skip to main content
SpringerLink
Log in

Gene mutation and DNA sequencing

  • Chapter
Handbook of Capillary Electrophoresis Applications

  • 738 Accesses

Abstract

Polymer sieving in capillary electrophoresis (CE) has been applied to DNA analysis because complementary double-stranded DNA or completely denatured single-stranded DNA molecules have a constant charge-to-mass ratio. These molecules migrate in sieving media, dependent only upon their molecular size, so that the migration velocity of the DNA of interest reflects its molecular size and secondary structure. Both gel-filled capillaries and replaceable linear polymer solutions can be used as sieving media. Polyacrylamide gel-filled capillaries can be prepared by procedures similar to conventional polyacrylamide slab gels, described in section 4.3. Gellike molecular sieving electrophoresis is performed in replaceable linear polymer solutions such as non-crosslinked polyacrylamide, polysaccharides, poly(ethylene glycol), etc. Recently replaceable linear polymer solutions have been employed more frequently than gel-filled capillaries because of their easy operation and highly reproducible measurements [1–3].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. McCord, B.R.; Jung, J.M.; Holleran, E.A. (1993) High resolution capillary electrophoresis of forensic DNA using a non-gel sieving buffer. J. Liq. Chromatogr. 16, 1963–1981.

    Article  CAS  Google Scholar 

  2. Williams, P.E.; Marino, M.A.; Del Rio, S.A.; Turni, L.A.; Devaney, J.M. (1994) Analysis of DNA restriction fragments and polymerase chain reaction products by capillary electrophoresis. J. Chromatogr. 680, 525–540.

    Article  CAS  Google Scholar 

  3. Wang, Y.; Ju, J.; Carpenter, B.A.; Atherton, J.M.; Sensabaugh, G.F.; Mathies, R.A. (1995) Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers. Anal. Chem. 67, 1197–1203.

    Article  CAS  Google Scholar 

  4. Nathakarnkitkool, S.; Oefner, P.J.; Bartsch, G; Chin, M.A.; Bonn, G.K. (1992) High-resolution capillary electrophoretic analysis of DNA in free solution. Electrophoresis 13, 18–31.

    Article  CAS  Google Scholar 

  5. Martin, F.; Vairelles, D.; Henrion, B. (1993) Automated ribosomal DNA fingerprinting by capillary electrophoresis of PCR products. Anal. Biochem. 214, 182–189.

    Article  CAS  Google Scholar 

  6. McCord, B.R.; McClure, D.L.; Jung, J.M. (1993) Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye. J. Chromatogr. 652, 75–82.

    Article  CAS  Google Scholar 

  7. Srinivasan, K.; Girard, J.E.; Williams, P.; Roby, R.K.; Weedn, V.W.; Morris, S.C.; Kline, M.C.; Reeder, D.J. (1993) Electrophoretic separations of polymerase chain reaction-amplified DNA fragments in DNA typing using a capillary electrophoresis-laser induced fluorescence system. J. Chromatogr. 652, 83–91.

    Article  CAS  Google Scholar 

  8. Felmlee, T.A.; Mitchell, P.S.; Ulfelder, K.J.; Persing, D.H.; Landers, J.P. (1995) Capillary electrophoresis for the post-amplification detection of a hepatitis C virus-specific DNA product in human serum. J. Capillary Electrophoresis 2, 125–130.

    CAS  Google Scholar 

  9. Butler, J.M.; McCord, B.R.; Jung, J.M.; Wilson, M.R.; Budowle, B.; Allen, R.O. (1994) Quantitation of polymerase chain reaction products by capillary electrophoresis using laser fluorescence. J. Chromatogr. 658, 271–280.

    CAS  Google Scholar 

  10. Lu, W.; Han, D.-S.; Yuan, J.; Andrieu, J.-M. (1994) Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence. Nature 368, 269–271.

    Article  CAS  Google Scholar 

  11. Ulfelder, K.J.; Schwartz, H.E.; Hall, J.M.; Sunzeri, F.J. (1992) Restriction fragment length polymorphism analysis of ERBB2 oncogene by capillary electrophoresis. Anal Biochem. 200, 260–267.

    Article  CAS  Google Scholar 

  12. Cheng, J.; Mitchelson, K.R. (1994) Glycerol-enhanced separation of DNA fragments in entangled solution capillary electrophoresis. Anal Chem. 66, 4210–4214.

    Article  CAS  Google Scholar 

  13. Chen, J.W.; Cohen, A.S.; Karger, B.L. (1991) Identification of DNA molecules by pre-column hybridization using capillary electrophoresis. J. Chromatogr. 559, 295–305.

    Article  CAS  Google Scholar 

  14. Mitchelson, K.R.; Cheng, J. (1995) Point mutation screening by high-performance capillary electrophoresis. J. Capillary Electrophoresis 2, 137–143.

    CAS  Google Scholar 

  15. Gelfi, C., Righetti, P.G.; Brancolini, V.; Cremonesi, L.; Ferrari, M. (1994) Capillary electrophoresis in polymer networks for analysis of PCR products: Detection of ΔF508 mutation in cystic fibrosis. Clin. Chem. 40, 1603–1605.

    CAS  Google Scholar 

  16. Gelfi, C.; Orsi, A.; Righetti, P.G.; Brancolini, V.; Cremonesi, L.; Ferrari, M. (1995) Capillary zone electrophoresis of polymerase chain reaction-amplified DNA fragments in polymer networks: The case of GATT microsatellites in cystic fibrosis. Electrophoresis 15, 640–643.

    Article  Google Scholar 

  17. Baba, Y.; Tomisaki, R.; Sumita, C.; Morimoto, I.; Sugita, S.; Tsuhako, M.; Miki, T.; Ogihara, T. (1995) Rapid typing of variable number of tandem repeat locus in the human apolipoprotein B gene for DNA diagnosis of heart disease by polymerase chain reaction and capillary electrophoresis. Electrophoresis 16, 1437–1440.

    Article  CAS  Google Scholar 

  18. Arakawa, H.; Uetanaka, K.; Maeda, M.; Tsuji, A.; Matsubara, Y.; Narisawa, K. (1994) Analysis of polymerase chain reaction-product by capillary electrophoresis with laser-induced fluorescence detection and its application to the diagnosis of medium-chain acylcoenzyme A dehydrogenase deficiency. J. Chromatogr. 680, 517–523.

    Article  CAS  Google Scholar 

  19. Arakawa, H.; Uetanaka, K.; Maeda, M.; Tsuji, A. (1994) Analysis of polymerase chain reaction product by capillary electrophoresis and its application to the detection of single base substitution in genes. J. Chromatogr. 664, 89–98.

    Article  CAS  Google Scholar 

  20. Del Principe, D.; Iampieri, M.P.; Germani, D.; Menichelli, A.; Novelli, G.; Dallapiccola, B. (1993) Detection by capillary electrophoresis of restriction fragment length polymorphism. Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene. J. Chromatogr. 638, 277–281.

    Article  Google Scholar 

  21. Guttman, A.; Nelson, R.J.; Cooke, N. (1992) Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis. J. Chromatogr. 593, 297–303.

    Article  CAS  Google Scholar 

  22. Satow, T.; Akiyama, T.; Machida, A.; Utagawa, Y.; Kobayashi, H. (1993) Simultaneous determination of the migration coefficient of each base in heterogeneous oligo-DNA by gel filled capillary electrophoresis. J. Chromatogr. 652, 23–30.

    Article  CAS  Google Scholar 

  23. Orita, M.; Iwahana, H.; Kanazawa, H.; Hayashi, K.; Sekiya, T. (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl Acad. Sci. USA 86, 2766–2770.

    Article  CAS  Google Scholar 

  24. Kuypers, A.W.H.M.; Willems, P.M.W.; van der Schans, M.J.; Linssen, P.C.M.; Wessels, H.M.C.; de Bruijn, C.H.M.M.; Everaerts, F.M.; Mensink, E.J.B.M. (1993) Detection of point mutations in DNA using capillary electrophoresis in a polymer network. J. Chromatogr. 621, 149–156.

    Article  CAS  Google Scholar 

  25. Hebenbrock, K.; Williams, P.M.; Karger, B.L. (1995) Single strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection. Electrophoresis 16, 1429–1436.

    Article  CAS  Google Scholar 

  26. Felmlee, T.A.; Oda, R.P.; Persing, D.A.; Landers, J.P. (1995) Capillary electrophoresis of DNA. Potential utility for clinical diagnoses. J. Chromatogr. 717, 127–137.

    Article  CAS  Google Scholar 

  27. Cheng, J.; Kasuga, T.; Mitchelson, K.R.; Lightly, E.R.T.; Watson, N.D.; Martin, W.J.; Atkinson, D. (1994) Polymerase chain reaction heteroduplex polymorphism analysis by entangled solution capillary electrophoresis. J. Chromatogr. 677, 169–177.

    Article  CAS  Google Scholar 

  28. Abrams, E.S.; Murdaugh, S.E.; Lerman, L.S. (1990) Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp. Genomics 7, 463–475.

    Article  CAS  Google Scholar 

  29. Khrapko, K.; Hanekamp, J.S.; Thilly, W.G.; Belenkii, A.; Foret, F.; Karger, B.L. (1994) Constant denaturant capillary electrophoresis (CDCE): a high resolution approach to mutational analysis. Nucleic Acids Res. 22, 364–369.

    Article  CAS  Google Scholar 

  30. Barany, F. (1991) Genetic disease detection and DNA amplification using cloned thermostable ligase. Proc. Natl Acad. Sci. USA 88, 189–193.

    Article  CAS  Google Scholar 

  31. Drossman, H.; Luckey, J.A.; Kostichka, A.J.; D’Cunha, J.; Smith, L.M. (1990) High-speed separations of DNA sequencing reactions by capillary electrophoresis. Anal. Chem. 62, 900–903.

    Article  CAS  Google Scholar 

  32. Cohen, A.S.; Najarian, D.R.; Karger, B.L. (1990) Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis. J. Chromatogr. 516, 49–60.

    Article  CAS  Google Scholar 

  33. Luckey, J.A.; Drossman, H.; Kostichka, A.J.; Mead, D.A.; D’Cunha, J.; Norris, T.B.; Smith, L.M. (1990) High speed DNA sequencing by capillary electrophoresis. Nucleic Acids Res. 18, 4417–4421.

    Article  CAS  Google Scholar 

  34. Tomisaki, R.; Baba, Y.; Tsuhako, M; Takahashi, S.; Murakami, K.; Anazawa, T.; Kambara, H. (1994) High-speed DNA sequencer using capillary gel electrophoresis with a laser-induced four-color fluorescent DNA detector. Anal. Sci. 10, 817–820.

    Article  CAS  Google Scholar 

  35. Swerdlow, H.; Zhang, J.Z.; Chen, D.Y.; Harke, H.R.; Grey, R.; Wu, S.; Dovichi, N.J.; Fuller, C. (1991) Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence. Anal. Chem. 63, 2835–2841.

    Article  CAS  Google Scholar 

  36. Swerdlow, H.; Wu, S.; Harke, H.R.; Dovichi, N.J. (1990) Capillary gel electrophoresis for DNA sequencing. Laser-induced fluorescence detection with the sheath flow cuvette. J. Chromatogr. 516, 61–67.

    Article  CAS  Google Scholar 

  37. Chen, D.Y.; Swerdlow, H.P.; Harke, H.R.; Zhang, J.Z.; Dovichi, N.J. (1991) Low-cost, high-sensitivity laser-induced fluorescence detection for DNA sequencing by capillary gel electrophoresis. J. Chromatogr. 559, 237–246.

    Article  CAS  Google Scholar 

  38. Luckey, J.A.; Smith, L.M. (1993) Optimization of electric field strength for DNA sequencing in capillary gel electrophoresis. Anal Chem. 65, 2841–2850.

    Article  CAS  Google Scholar 

  39. Swerdlow, H.; Dew-Jager, K.E.; Brady, K.; Grey, R.; Dovichi, N.J.; Gesteland, R. (1992) Stability of capillary gels for automated sequencing of DNA. Electrophoresis 13, 475–483.

    Article  CAS  Google Scholar 

  40. Rocheleau, M.J.; Grey, R.J.; Chen, D.Y.; Harke, H.R.; Dovichi, N.J. (1992) Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis. Electrophoresis 13, 484–486.

    Article  CAS  Google Scholar 

  41. Ruiz-Martinez, M.C.; Berka, J.; Belenkii, A.; Foret, F.; Miller, A.W.; Karger, B.L. (1993) DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection. Anal. Chem. 65, 2851–2858.

    Article  CAS  Google Scholar 

  42. Chen, N.; Manabe, T.; Terabe, S.; Yohda, M.; Endo, I. (1994) High-resolution separation of oligonucleotides and DNA sequencing reaction products by capillary electrophoresis with linear polyacrylamide and laser-induced fluorescence detection. J. Microcol. Sep. 6, 539–543.

    Article  CAS  Google Scholar 

  43. Manabe, T.; Chen, N.; Terabe, S.; Yohda, M.; Endo, I. (1994) Effects of linear polyacrylamide concentrations and applied voltages on the separation of oligonucleotides and DNA sequencing fragments by capillary electrophoresis. Anal. Chem. 66, 4243–4252.

    Article  CAS  Google Scholar 

  44. Zhang, J.Z.; Fang, Y.; Hou, J.Y.; Ren, H.J.; Jiang, R.; Roos, P.; Dovichi, N.J. (1995) Use of non-cross-linked polyacrylamide for four-color DNA sequencing by capillary electrophoresis separation of fragments up to 640 bases in length in two hours. Anal. Chem. 67, 4587–593.

    Google Scholar 

  45. Fung, E.N.; Yeung, E.S. (1995) High-speed DNA sequencing by using mixed poly(ethylene oxide) solutions in uncoated capillary columns. Anal. Chem. 67, 1913–1919.

    Article  CAS  Google Scholar 

  46. Huang, X.C.; Quesada, M.A.; Mathies, R.A. (1992) Capillary array electrophoresis using laser-excited confocal fluorescence detection. Anal. Chem. 64, 967–972.

    Article  CAS  Google Scholar 

  47. Taylor, J.A.; Yeung, E.A. (1993) Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination. Anal. Chem. 65, 956–960.

    Article  CAS  Google Scholar 

  48. Ueno, K.; Yeung, E.S. (1994) Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries. Anal. Chem. 66, 1424–1431.

    Article  CAS  Google Scholar 

  49. Takahashi, S.; Murakami, K.; Anazawa, T.; Kambara, H. (1994) Multiple sheath-flow gel capillary-array electrophoresis for multicolor fluorescent DNA detection. Anal. Chem. 66, 1021–1026.

    Article  CAS  Google Scholar 

  50. Effenhauser, C.S.; Paulus, A.; Manz, A.; Widmer, H.M. (1994) High-speed separation of antisense oligonucleotides on a micromachined capillary electrophoresis device. Anal. Chem. 66, 2949–2953.

    Article  CAS  Google Scholar 

  51. Woolley, A.T.; Mathies, R.A. (1994) Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips. Proc. Natl Acad. Sci. USA 91, 11348–11352.

    Article  CAS  Google Scholar 

  52. Woolley, A.T.; Mathies, R.A. (1995) Ultra-high-speed DNA sequencing using capillary electrophoresis chips. Anal. Chem. 67, 3676–3680.

    Article  CAS  Google Scholar 

Download references

Authors
  1. K. Kitagishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Chapman & Hall

About this chapter

Cite this chapter

Kitagishi, K. (1997). Gene mutation and DNA sequencing. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_32

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-1561-9_32

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation