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Analysis of nucleic acids by capillary electrophoresis

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Handbook of Capillary Electrophoresis Applications

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Abstract

This chapter gives descriptions of the separation of bases, substituted bases, nucleosides, nucleotides, oligonucleotides, DNA and RNA. The separation modes for these compounds are included in the text as well as in Table 21.1.

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References

  1. Lee, K.-J.; Heo, G.S.; Kim, N.J.; Moon, D.-C. (1992) Separation of theophylline and its analogues by micellar electrokinetic chromatography: application to the determination of theophylline in human plasma. J. Chromatogr. 577, 135–141.

    Article  CAS  Google Scholar 

  2. Thormann, W.; Minger, A.; Molteni, S.; Caslavska, J.; Gebauer, P. (1992) Determination of substituted purines in body fluids by micellar electrokinetic capillary chromatography with direct sample injection. J. Chromatogr. 593, 275–288.

    Article  CAS  Google Scholar 

  3. Singhal, R.P.; Hughbanks, D.; Xian, J. (1992) Separation of dideoxyribonucleosides in trace amounts by automated liquid chromatography and capillary electrophoresis. J. Chromatogr. 609, 147–161.

    Article  CAS  Google Scholar 

  4. Kaneta, T.; Tanaka, S.; Taga, M.; Yoshida, H. (1992) Effect of addition of glucose on micellar electrokinetic capillary chromatography with sodium dodecyl sulphate. J. Chromatogr. 609, 369–374.

    Article  CAS  Google Scholar 

  5. Li, W.; Moussa, A.; Giese, R.W. (1992) Capillary electrophoresis of fluorescein-ethylenediamine-5′-deoxynucleotides. J. Chromatogr. 608, 171–174.

    Article  CAS  Google Scholar 

  6. Li, W.; Moussa, A.; Giese, R.W. (1993) Capillary electrophoresis with laser fluorescence detection for profiling damage to fluorescein-labeled deoxyadenylic acid by background, ionizing radiation and hydrogen peroxide. J. Chromatogr. 633, 315–319.

    Article  CAS  Google Scholar 

  7. Shao, X.; O’Neill, K.; Zhao, Z.; Anderson, S.; Malik, A.; Lee, M. (1994) Analysis of nucleotide pools in human lymphoma cells by capillary electrophoresis. J. Chromatogr. 680, 463–468.

    Article  CAS  Google Scholar 

  8. Norwood, C.B.; Jackim, E.; Cheer, S. (1993) DNA adduct research with capillary electrophoresis. Anal. Biochem. 213, 194–199.

    Article  CAS  Google Scholar 

  9. Harvey, S.D.; Bean, R.M.; Udseth, H.R. (1992) High-resolution separation and detection of DNA adducts of benzo[a]pyrene. J. Microcol. Sep. 4, 191–198.

    Article  CAS  Google Scholar 

  10. Terabe, S.; Chen, N.; Otsuka, K. (1994) Micellar electrokinetic chromatography. In Advances in Electrophoresis, Chrambach, A.; Dunn, M.J.; Radola, B.J. (eds), VCH, Weinheim, Germany, Vol. 7, pp. 87–153.

    Google Scholar 

  11. Srivatsa, G.S.; Batt, M.; Schuette, J.; Carlson, R.H.; Fitchett, J.; Lee, C; Cole, D.L. (1994) Quantitative capillary gel electrophoresis assay of phosphorothioate oligonucleotides in pharmaceutical formulations. J. Chromatogr. 680, 469–477.

    Article  CAS  Google Scholar 

  12. Guttman, A.; Nelson, R.J.; Cooke, N. (1992) Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis. J. Chromatogr. 593, 297–303.

    Article  CAS  Google Scholar 

  13. Satow, T.; Akiyama, T.; Machida, A.; Utagawa, Y.; Kobayashi, H. (1993) Simultaneous determination of the migration coefficient of each base in heterogeneous oligo-DNA by gel filled capillary electrophoresis. J. Chromatogr. 652, 23–30.

    Article  CAS  Google Scholar 

  14. Baba, Y.; Tsuhako, M.; Sawa, T.; Akashi, M.; Yashima, E. (1992) Specific base recognition of oligodeoxynucleotides by capillary affinity gel electrophoresis using polyacrylamide-poly(9-vinyladenine) conjugated gel. Anal. Chem. 64, 1920–1925.

    Article  CAS  Google Scholar 

  15. Akashi, M.; Sawa, T.; Baba, Y.; Tsuhako, M. (1992) Specific separation of oligodeoxynucleotides by capillary affinity gel electrophoresis (CAGE) using poly(9-vinyladenine)-polyacrylamide conjugated gel. J. High Resolut. Chromatogr. 15, 625–626.

    Article  CAS  Google Scholar 

  16. Baba, Y.; Tsuhako, M.; Sawa, T.; Akashi, M. (1993) Temperature-programmed capillary affinity gel electrophoresis for the sensitive base-specific separation of oligodeoxynucleotides. J. Chromatogr. 632, 137–142.

    Article  CAS  Google Scholar 

  17. Bevan, CD.; Mutton, I.M.; Pipe, A.J. (1993) Resolution of diastereomeric phosphoramidate bridged unnatural oligonucleotides by micellar electrokinetic chromatography. J. Chromatogr. 636, 113–123.

    Article  CAS  Google Scholar 

  18. Cohen, A.S.; Najarian, D.; Smith, J.A.; Karger, B.L. (1988) Rapid separation of DNA restriction fragments using capillary electrophoresis. J. Chromatogr. 458, 323–333.

    Article  CAS  Google Scholar 

  19. Martin, F.; Vairelles, D.; Henrion, B. (1993) Automated ribosomal DNA fingerprinting by capillary electrophoresis of PCR products. Anal. Biochem. 214, 182–189.

    Article  CAS  Google Scholar 

  20. Grossman, P.D.; Soane, D.S. (1991) Capillary electrophoresis of DNA in entangled polymer solutions. J. Chromatogr. 559, 257–266.

    Article  CAS  Google Scholar 

  21. Luckey, J.A.; Smith, L.M. (1993) A model for the mobility of single-stranded DNA in capillary gel electrophoresis. Electrophoresis 14, 492–501.

    Article  CAS  Google Scholar 

  22. McCord, B.R.; Jung, J.M.; Holleran, E.A. (1993) High resolution capillary electrophoresis of forensic DNA using a non-gel sieving buffer. J. Liq. Chromatogr. 16, 1963–1981.

    Article  CAS  Google Scholar 

  23. MacCrehan, W.A.; Rasmussen, H.T.; Northrop, D.M. (1992) Size-selective capillary electrophoresis (SSCE) separation of DNA fragments. J. Liq. Chromatogr. 15, 1063–1080.

    Article  CAS  Google Scholar 

  24. Ulfelder, K.J.; Schwartz, H.E.; Hall, J.M.; Sunzeri, F.J. (1992) Restriction fragment length polymorphism analysis ERBB2 oncogene by capillary electrophoresis. Anal. Biochem. 200, 260–267.

    Article  CAS  Google Scholar 

  25. Cheng, J.; Mitchelson, K.R. (1994) Glycerol-enhanced separation of DNA fragments in entangled solution capillary electrophoresis. Anal. Chem. 66, 4210–214.

    Article  CAS  Google Scholar 

  26. Williams, P.E.; Marino, M.A.; Del Rio, S.A.; Turni, L.A.; Devaney, J.M. (1994) Analysis of DNA restriction fragments and polymerase chain reaction products by capillary electrophoresis. J. Chromatogr. 680, 525–540.

    Article  CAS  Google Scholar 

  27. McCord, B.R.; McClure, D.L.; Jung, J.M. (1993) Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye. J. Chromatogr. 652, 75–82.

    Article  CAS  Google Scholar 

  28. Srinivasan, K.; Girard, J.E.; Williams, P.; Roby, R.K.; Weedn, V.W.; Morris, S.C.; Kline, M.C.; Reeder, D.J. (1993) Electrophoretic separations of polymerase chain reaction-amplified DNA fragments in DNA typing using a capillary electrophoresis-laser induced fluorescence system. J. Chromatogr. 652, 83–91.

    Article  CAS  Google Scholar 

  29. Zhu, H.; Clark, S.M.; Benson, S.C.; Rye, H.S.; Glazer, A.N.; Mathies, R.A. (1994) High-sensitivity capillary electrophoresis of double-stranded DNA fragments using monomeric and dimeric fluorescent intercalating dyes. Anal. Chem. 66, 1941–1948.

    Article  CAS  Google Scholar 

  30. Butler, J.M.; McCord, B.R.; Jung, J.M.; Wilson, M.R.; Budowle, B.; Allen, R.O. (1994) Quantitation of polymerase chain reaction products by capillary electrophoresis using laser fluorescence. J. Chromatogr. 658, 271–280.

    CAS  Google Scholar 

  31. Hebenbrock, K.; Williams, P.M.; Karger, B.L. (1995) Single strand conformational polymorphism using capillary electrophoresis with two-dye laser-induced fluorescence detection. Electrophoresis 16, 1429–1436.

    Article  CAS  Google Scholar 

  32. Wang, Y.; Ju, J.; Carpenter, B.A.; Atherton, J.M.; Sensabaugh, G.F.; Mathies, R.A. (1995) Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers. Anal Chem. 67, 1197–1203.

    Article  CAS  Google Scholar 

  33. Figeys, D.; Renborg, A.; Dovichi, N.J. (1994) Labeling of double-stranded DNA by ROX-dideoxycytosine triphosphate using terminal deoxynucleotidyl transferase and separation by capillary electrophoresis. Anal. Chem. 66, 4382–4383.

    Article  CAS  Google Scholar 

  34. Luckey, J.A.; Drossman, H.; Kostichka, A.J.; Mead, D.A.; D’Cunha, J.; Norris, T.B.; Smith, L.M. (1990) High speed DNA sequencing by capillary electrophoresis. Nucleic Acids Res. 18, 4417–4421.

    Article  CAS  Google Scholar 

  35. Luckey, J.A.; Norris, T.B.; Smith, J.M. (1993) Analysis of resolution in DNA sequencing by capillary gel electrophoresis. J. Phys. Chem. 97, 3067–3075.

    Article  CAS  Google Scholar 

  36. Cohen, A.S.; Najarian, D.R.; Karger, B.L. (1990) Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis. J. Chromatogr. 516, 49–60.

    Article  CAS  Google Scholar 

  37. Chen, D.Y.; Swerdlow, H.P.; Harke, H.R.; Zhang, J.Z.; Dovichi, N.J. (1991) Low-cost, high-sensitivity laser-induced fluorescence detection for DNA sequencing by capillary gel electrophoresis. J. Chromatogr. 559, 237–246.

    Article  Google Scholar 

  38. Manabe, T.; Chen, N.; Terabe, S.; Yohda, M.; Endo, I. (1994) Effects of linear polyacrylamide concentrations and applied voltages on the separation of oligonucleotides and DNA sequencing fragments by capillary electrophoresis. Anal. Chem. 66, 4243–4252.

    Article  Google Scholar 

  39. Fung, E.N.; Yeung, E.S. (1995) High-speed DNA sequencing by using mixed poly(ethylene oxide) solutions in uncoated capillary columns. Anal. Chem. 67, 1913–1919.

    Article  Google Scholar 

  40. Nathakarnkitkool, S.; Oefner, P.J.; Bartsch, G.; Chin, M.A.; Bonn, G.K. (1992) High-resolution capillary electrophoretic analysis of DNA in free solution. Electrophoresis 13, 18–31.

    Article  Google Scholar 

  41. Felmlee, T.A.; Mitchell, P.S.; Ulfelder, K.J.; Persing, D.H.; Landers, J.P. (1995) Capillary electrophoresis for the post-amplification detection of a hepatitis C virus-specific DNA product in human serum. J. Capillary Electrophoresis 2, 125–130.

    Google Scholar 

  42. Lu, W.; Han, D.-S.; Yuan, J.; Andrieu, J.-M. (1994) Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence. Nature 368, 269–271.

    Article  Google Scholar 

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Authors
  1. K. Kitagishi
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Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Kitagishi, K. (1997). Analysis of nucleic acids by capillary electrophoresis. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_22

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_22

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

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