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Analysis of antibodies by capillary electrophoresis

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Handbook of Capillary Electrophoresis Applications

Abstract

High resolution analysis of antibodies can be one of the more difficult challenges that the analytical biochemist faces. The high molecular weight of intact antibodies makes the use of reverse-phase HPLC (high performance liquid chromatography), one of the highest resolution tools in the analyst’s repertoire, extremely difficult if not impossible. In addition, since many antibodies differ only slightly in amino acid content and molecular weight, size-based separations are often of limited use. To complicate matters further, antibodies are glycosylated and otherwise modified post-translationally, meaning that actual samples will exist as a population of closely related species all of which must be separated for a thorough analvsis.

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Authors
  1. T. Pritchett
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Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Pritchett, T. (1997). Analysis of antibodies by capillary electrophoresis. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_19

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_19

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

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