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Handbook of Capillary Electrophoresis Applications pp 207–218Cite as

Assay of enzymes by capillary electrophoresis

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Abstract

Enzymes are mostly proteins with specific catalytic activity corresponding to their substrates. They are essential components in living cells and a substantial part of modern biochemistry and molecular biology is devoted to their study. Nearly all biochemical reactions occur in complex mixtures with great speed through the mediation of enzymes with a high degree of specificity and great efficiency. In contrast to an antibody which binds specifically to the ground state of a molecule, it is generally recognized that enzymes bind to a transition state of a chemical reaction, which lowers the activation energy of the reaction pathway and consequently significantly increases the reaction rate. The catalytic efficiency of most enzymes is extremely high such that on a molar basis, they can catalyze the transformation of 103 to 106 moles of the substrate per minute. Enzymes provide some of the best chemical amplification processes in nature which have been used in many practical applications, including enzyme assay, enzyme-linked immunoassay (ELISA) and DNA probe assays.

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Authors
  1. F.-T. A. Chen
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  2. R. A. Evangelista
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Editor information

Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Chen, FT.A., Evangelista, R.A. (1997). Assay of enzymes by capillary electrophoresis. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_17

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

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