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Handbook of Capillary Electrophoresis Applications pp 158–172Cite as

Sodium dodecyl sulfate (SDS)-non-gel molecular sieving capillary electrophoresis for proteins

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Abstract

The long monopoly enjoyed by polyacrylamide slab gel electrophoresis in protein separation by molecular size is finally coming to the end as the novel method of capillary electrophoresis comes to the fore [1, 2]. There are three reasons why gel packed in a glass tube or between glass plates has long been used for separation:

  1. 1

    heat convection can be suppressed;

  2. 2

    electroosmosis can be suppressed;

  3. 3

    separation by molecular size can be realized.

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References

  1. Heller, C. (1995) Capillary electrophoresis of proteins and nucleic acids in gels and entangled polymer solution. J. Chromatogr. 698, 19–31.

    Article  CAS  Google Scholar 

  2. Takagi, T.; Karim, M.R. (1994) SDS-polymer solution capillary electrophoresis. Tanpakushitu Kakusan Koso. 39, 930–940.

    CAS  Google Scholar 

  3. Grossman, P.D.; Soane, D.S. (1991) Capillary electrophoresis of DNA in entangled polymer solutions. J. Chromatogr. 559, 257–266.

    Article  CAS  Google Scholar 

  4. Bae, Y.C.; Soane, D. (1993) Polymeric separation media for electrophoresis: cross-linked systems or entangled solutions. J. Chromatogr. 652, 17–22.

    Article  CAS  Google Scholar 

  5. Liu, J.; Dolink, V.; Hsieh, Y.; Novotny, M. (1992) Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns. Anal. Chem. 64, 1328–1336.

    Article  CAS  Google Scholar 

  6. Cohen, A.S.; Karger, B.L. (1987) High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins. J. Chromatogr. 397, 409–417.

    Article  CAS  Google Scholar 

  7. Wu, D.; Regnier, E. (1993) Native protein separations and enzyme microassays by capillary zone and gel electrophoresis. Anal. Chem. 65, 2029–2035.

    Article  CAS  Google Scholar 

  8. Tsuji, K. (1994) Factors affecting the performance of sodium dodecyl sulfate gel-filled capillary electrophoresis. J. Chromatogr. 661, 257–264.

    Article  CAS  Google Scholar 

  9. Heiger, D.N.; Cohen, A.S.; Karger, B.L. (1990) Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields. J. Chromatogr. 516, 33–48.

    Article  CAS  Google Scholar 

  10. Cordier, Y.; Roch, O.; Cordier, P.; Bischoff, R. (1994) Capillary gel electrophoresis of oligonucleotides: prediction of migration times using base-specific migration coefficients. J. Chromatogr. 680, 479–489.

    Article  CAS  Google Scholar 

  11. Gelfi, C; Alloni, A.; Besi, P.; Righetti, P.G. (1992) Investigation of the properties of acrylamide bifunctional monomers (cross-linkers) by capillary zone electrophoresis. J. Chromatogr. 608, 343–348.

    Article  CAS  Google Scholar 

  12. Righetti, P.G.; Chiari, M.; Nesi, M.; Caglio, S. (1993) Towards new formulations for polyacrylamide matrices, as investigated by capillary zone electrophoresis. J. Chromatogr. 638, 165–178.

    Article  CAS  Google Scholar 

  13. Gelfi, C.; Besi, P.; Alloni, A.; Righetti, P.G. (1992) Investigation of the properties of novel acrylamide monomers by capillary zone electrophoresis. J. Chromatogr. 608, 333–341.

    Article  CAS  Google Scholar 

  14. Chiari, M.; Nesi, M.; Righetti, P.G. (1993) Movement of DNA fragments during capillary zone electrophoresis in liquid polyacrylamide. J. Chromatogr. 652, 31–39.

    Article  CAS  Google Scholar 

  15. Singhal, R.P.; Xian, J. (1993) Separation of DNA restriction fragments by polymer-solution capillary zone electrophoresis. J. Chromatogr. 652, 47–56.

    Article  CAS  Google Scholar 

  16. Kleparnik, K.; Garner, M.; Bocek, P. (1995) Injection bias of DNA fragments in capillary electrophoresis with sieving. J. Chromatogr. 698, 375–383.

    Article  CAS  Google Scholar 

  17. Gelfi, C.; Besi, P.D.; Alloni, A.; Righetti, P.G.; Lyubimova, T.; Briskman, V.A. (1992) Kinetics of acrylamide photopolymerization as investigated by capillary zone electrophoresis. J. Chromatogr. 598, 277–285.

    Article  CAS  Google Scholar 

  18. Rapp, T.L.; Kowalchyk, W.K.; Davis, K.L.; Todd, E.A.; Liu, K.L.; Morris, M.D. (1992) Acrylamide polymerization kinetics in gel electrophoresis capillaries. A Raman micro-probe study. Anal. Chem. 64, 2434–2437.

    Article  CAS  Google Scholar 

  19. Guttman, A.; Horvath, J.; Cooke, N. (1993) Influence of temperature on the sieving effect of different polymer matrices in capillary SDS gel electrophoresis of proteins. Anal. Chem. 65, 199–203.

    Article  CAS  Google Scholar 

  20. Nakatani, M.; Shibukawa, A.; Nakagawa, T. (1994) Preparation and characterization of a stable polyacrylamide sieving matrix-filled capillary for high-performance capillary electrophoresis. J. Chromatogr. 661, 315–321.

    Article  CAS  Google Scholar 

  21. Cobb, K.A.; Dolnik, V.; Novotny, M. (1990) Electrophoretic separations of proteins in capillaries with hydrolytically stable surface structures. Anal. Chem. 62, 2478–2483.

    Article  CAS  Google Scholar 

  22. Izumi, T.; Yamaguchi, M.; Yoneda, K.; Isobe, T.; Okuyama, T.; Shinoda, T. (1993) Use of glucomannan for the separation of DNA fragments by capillary electrophoresis. J. Chromatogr. 652, 41–46.

    Article  CAS  Google Scholar 

  23. Grossman, P.D.; Hino, T.; Soane, D.S. (1992) Dynamic light-scattering studies of hydroxyethyl cellulose solutions used as sieving media for electrophoretic separations. J. Chromatogr. 608, 79–83.

    Article  CAS  Google Scholar 

  24. Yin, H.F.; Lux, J.A.; Schomburg, G. (1990) Production of polyacrylamide gel filled capillaries for capillary gel electrophoresis (CGE): Influence of capillary surface pretreatment on performance and stability. J. High Resolut. Chromatogr. 13, 624–627.

    Article  CAS  Google Scholar 

  25. Chang, H.T.; Yeung, E.S. (1995) Poly(ethylene oxide) for high-resolution and high-speed separation of DNA by capillary electrophoresis. J. Chromatogr. 669, 113–123.

    Article  CAS  Google Scholar 

  26. Baba, Y.; Ishimaru, N.; Samata, K.; Tsuhako, M. (1993) High-resolution separation of DNA restriction fragments by capillary electrophoresis in cellulose derivative solutions. J. Chromatogr. 653, 329–335.

    Article  CAS  Google Scholar 

  27. McCord, B.R.; Jung, J.M.; Holleran, E A. (1993) High resolution capillary electrophoresis of forensic DNA using a non-gel sieving buffer. J. Liq. Chromatogr. 16, 1963–1981.

    Article  CAS  Google Scholar 

  28. Chan, K.C.; Whang, C.W.; Yeung, E.S. (1993) Separation of DNA restriction fragments using capillary electrophoresis. J. Liq. Chromatogr. 16, 1941–1962.

    Article  CAS  Google Scholar 

  29. Barron, A.E.; Soane, D.S.; Blanch, H.W. (1993) Capillary electrophoresis of DNA in uncross-linked polymer solutions. J. Chromatogr. 652, 3–16.

    Article  CAS  Google Scholar 

  30. McGregor, D. A.; Yeung, E.S. (1993) Optimization of capillary electrophoretic separation of DNA fragments based on polymer filled capillaries. J. Chromatogr. 652, 67–73.

    Article  CAS  Google Scholar 

  31. Guttman, A.; Schwartz, H.E. (1995) Artifacts related to sample introduction in capillary gel electrophoresis affecting separation performance and quantitation. Anal. Chem. 67, 2279–2283.

    Article  CAS  Google Scholar 

  32. Gelfi, C; Orsi, A.; Leoncini, F.; Righetti, P.G. (1995) Fluidified polyacrylamides as molecular sieves in capillary zone electrophoresis of DNA fragments. J. Chromatogr. 689, 97–105.

    Article  CAS  Google Scholar 

  33. Martinez, M.C.R.; Berka, J.; Belenkii, A.; Foret, F.; Miller, A.W.; Karger, B.L. (1993) DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection. Anal. Chem. 65, 2851–2858.

    Article  Google Scholar 

  34. Schans, M.J.; Allen, K.A.; Wanders, B.J.; Guttman, A. (1994) Effects of sample matrix and injection plug on dsDNA migration in capillary gel electrophoresis. J. Chromatogr. 680, 511–516.

    Article  Google Scholar 

  35. Best, N.; Arriaga, E.; Chen, D.Y.; Dovichi, N. (1994) Separation of fragments up to 570 bases in length by use of 6% T non-cross-linked polyacrylamide for DNA sequencing in capillary electrophoresis. Anal. Chem. 66, 4063–4067.

    Article  CAS  Google Scholar 

  36. Pariat, Y.F.; Berka, J.; Heiger, D.N.; Schmit, T.; Vilenchik, M.; Cohen, A.S.; Foret, F.; Karger, B.L. (1993) Separation of DNA fragments by capillary electrophoresis using replaceable linear polyacrylamide matrices. J. Chromatogr. 652, 57–66.

    Article  CAS  Google Scholar 

  37. Cheng, J.; Mitchelson, K.R. (1994) Glycerol-enhanced separation of DNA fragments in entangled solution capillary electrophoresis. Anal. Chem. 66, 4210–4214.

    Article  CAS  Google Scholar 

  38. Manabe, T.; Chen, N.; Terabe, S.; Yohda, M.; Endo, I. (1994) Effects of linear polyacrylamide concentrations and applied voltages on the separation of oligonucleotides and DNA sequencing fragments by capillary electrophoresis. Anal. Chem. 66, 4243–4252.

    Article  CAS  Google Scholar 

  39. Mitnik, L.; Salome, L.; Viovy, J.L.; Heller, C. (1995) Systematic study of field and concentration effects in capillary electrophoresis of DNA in polymer solutions. J. Chromatogr. 710, 309–321.

    Article  CAS  Google Scholar 

  40. Widhalm, A.; Schwer, C; Blaas, D.; Kenndler, E. (1991) Capillary zone electrophoresis with a linear, non-cross-linked polyacrylamide gel: separation of proteins according to molecular mass. J. Chromatogr. 549, 446–451.

    Article  CAS  Google Scholar 

  41. Wu, D.; Regnier, F.E. (1992) Sodium dodecyl sulfate-capillary gel electrophoresis of proteins using non-cross-linked polyacrylamide. J. Chromatogr. 604, 349–356.

    Article  Google Scholar 

  42. Werner, W.E.; Demorest, D.M.; Stevens, J.; Wiktorowicz, J.E. (1993) Size-dependent separation of proteins denatured in SDS by capillary electrophoresis using a replaceable sieving matrix. Anal. Biochem. 212, 253–258.

    Article  CAS  Google Scholar 

  43. Guttman, A.; Shieh, P.; Lindahl, J.; Cooke, N. (1994) Capillary sodium dodecyl sulfate gel electrophoresis of proteins II. On the Ferguson method in polyethylene oxide gels. J. Chromatogr. 676, 227–231.

    Article  CAS  Google Scholar 

  44. Benedek, K.; Guttman, A. (1994) Ultra-fast high-performance capillary sodium dodecyl sulfate gel electrophoresis of proteins. J. Chromatogr. 680, 375–381.

    Article  CAS  Google Scholar 

  45. Guttman, A.; Nolan, A.N.; Cooke, N. (1993) Capillary sodium dodecyl sulfate gel electrophoresis of proteins. J. Chromatogr. 632, 171–175.

    Article  CAS  Google Scholar 

  46. Lauch, T.; Scheper, T.; Reif, O.-W.; Fleisher, J.; Freitag, R. (1993) Rapid capillary gel electrophoresis of proteins. J. Chromatogr. 654, 190–195.

    Article  Google Scholar 

  47. Ganzler, K.; Greve, K.S.; Cohen, A.S.; Karger, B.L.; Guttmann, A.; Cooke, N.C. (1992) High-performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks. Anal. Chem. 64, 2665–2671.

    Article  CAS  Google Scholar 

  48. Guttman, A.; Horvath, J.; Cooke, N. (1993) Influence of temperature on the sieving effect of different polymer matrices in capillary SDS gel electrophoresis of proteins. Anal. Chem. 65, 199–203.

    Article  CAS  Google Scholar 

  49. Alfonso, E.S.; Conti, M.; Gelgi, C.; Righetti, P.G. (1995) Sodium dodecyl sulfate capillary electrophoresis of proteins in entangled solutions of poly(vinyl alcohol). J. Chromatogr. 689, 85–96.

    Article  Google Scholar 

  50. Deyl, Z.; Miksik, I. (1995) Separation of collagen type I chain polymers by electrophoresis in non-cross-linked polyacrylamide-filled capillaries. J. Chromatogr. 698, 369–373.

    Article  CAS  Google Scholar 

  51. Reif, O.W.; Freitag, R. (1994) Control of the cultivation process of antithrombin II and its characterization by capillary electrophoresis. J. Chromatogr. 680, 383–394.

    Article  CAS  Google Scholar 

  52. Kinghorn, N.M.; Norris, C.S.; Paterson, G.R.; Otter, D.E. (1995) Comparison of capillary electrophoresis with traditional methods to analyse bovine whey proteins. J. Chromatogr. 700, 111–123.

    Article  CAS  Google Scholar 

  53. Tsuji, K. (1993) Evaluation of sodium dodecyl sulfate non-acrylamide polymer gel-filled capillary electrophoresis for molecular size separation of recombinant bovine somatotropin. J. Chromatogr. 652, 139–147.

    Article  CAS  Google Scholar 

  54. Manabe, T. (1994) Capillary electrophoresis: application for biopolymers. Bunseki, 467–472.

    Google Scholar 

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Authors
  1. Y. Kurosu
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  2. M. Saito
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Editor information

Editors and Affiliations

  1. National Institute of Health Sciences, Ministry of Health & Welfare, 1-18-1, Kamiyoga, Setagaya, Tokyo, Japan

    Hideharu Shintani (Section Director) (Section Director)

  2. Slovak Technical University, Bratislava, Slovakia

    J. Polonský (Professor of Analytical Chemistry) (Professor of Analytical Chemistry)

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© 1997 Chapman & Hall

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Kurosu, Y., Saito, M. (1997). Sodium dodecyl sulfate (SDS)-non-gel molecular sieving capillary electrophoresis for proteins. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_14

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  • DOI: https://doi.org/10.1007/978-94-009-1561-9_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7197-0

  • Online ISBN: 978-94-009-1561-9

  • eBook Packages: Springer Book Archive

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