Abstract
The technology for introducing chimeric DNA constructs into cultured cells provides an important new approach for analysing diverse aspects of gene expression. In particular, transfection studies with cell lines from mosquitoes are beginning to provide insights into the structural and functional features of genes, and their regulatory elements that play essential roles in the physiological processes that maintain disease transmission cycles. Functional assays with transfected mosquito cells will further enable the investigator to develop novel approaches, such as antisense nucleic acid technologies and suicide selection systems, whose implementation has potential applications for molecular disruption of disease transmission in transgenic mosquitoes.
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Fallon, A.M. (1997). Transfection of cultured mosquito cells . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_36
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DOI: https://doi.org/10.1007/978-94-009-1535-0_36
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