Abstract
In 1968, Britten and Kohne introduced C0t curve analysis to examine the reassociation kinetics of genomic DNA. Using this technique they were able to estimate genome size and, more importantly, demonstrate that eukaryotic genomes consist of a complex mixture of repetitive and unique DNA sequences. In addition to protein coding genes, genomes contain large blocks of noncoding sequences, some of which are repetitive. Repetitive DNA is categorized as either highly repetitive (∼1000 – 100 000 copies) or moderately repetitive (2–∼1000 copies) (reviewed in Haiti, 1991; Lewin, 1990).
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Palmer, M.J., Black, W.C. (1997). Determination of insect genome size and complexity by reassociation kinetics . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_16
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DOI: https://doi.org/10.1007/978-94-009-1535-0_16
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