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Mass spectrometry of sterols

  • Chapter
Analysis of Sterols

Abstract

The potential for the application of mass spectrometry (MS), and especially combined gas chromatography—mass spectrometry (GC—MS), to sterol and steroid analysis was recognized in the 1960s [1–12]. Since that time the applications of mass spectroscopy for sterol analysis have moved in parallel with the considerable advances in instrument design and the development of new techniques. Mass spectrometry, and GC—MS in particular, is an ideally suited method to help with the unravelling of the identities of the components in a complex sterol mixture. Among the factors which have made GC—MS such a good analytical method for sterol analysis are: (a) the high sensitivity of the technique; (b) the excellent resolutions of closely related sterols now achievable on fused-silica bonded stationary-phase capillary gas chromatography columns that are capable of high-temperature operation and therefore also suited to steryl conjugate analysis; (c) improved designs for interfacing the GC with the mass spectrometer ensuring good sample transfer; (d) powerful computer programs for instrument operation and data handling that allow mass chromatography, selected ion monitoring, library searching of spectra, etc.; (e) the introduction of bench-top quadrupole and ion-trap mass spectrometers with automatic sample injection systems which are now permitting GC—MS to become a routine laboratory technique. The development of alternative ionization methods has also benefited the analysis of steryl conjugates which were previously rather intractable to MS examination. The mass spectrometry of sterols and related compounds has been reviewed in books and articles [1, 13–20].

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Authors and Affiliations

  1. School of Biological Sciences, University of Liverpool, UK

    L. John Goad

  2. College of Science and Technology, Nihon University, Tokyo, Japan

    Toshihiro Akihisa

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© 1997 Chapman & Hall

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Goad, L.J., Akihisa, T. (1997). Mass spectrometry of sterols. In: Analysis of Sterols. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1447-6_7

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