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Extraction of sterols from tissues

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Analysis of Sterols

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Abstract

The ideal method to utilize for the extraction of lipid from a tissue should be one which will remove all the required lipophilic compounds efficiently without losses or artefact formation due to hydrolysis, autoxidation or other degradation. The most well-established solvent extraction-partitioning methods were those developed originally for animal tissues by Folch et al. [1] and Bligh and Dyer [2]. They are still widely applied to obtain lipids from tissues of animals and have also been adopted by many workers for the extraction of lipids from micro-organisms and plants. These methods are based upon the ability of chloroform—methanol (CHCl3—MeOH) mixtures to form a monophasic system with the water in the tissue which will then disrupt the membrane structures and remove the lipid from the tissues. In addition to these two methods, various other solvent systems and techniques are used for the extraction of sterols from tissues and cells of plant and animal origins. Table 2-1 lists examples of the methods of lipid extraction employed with plant or animal materials, and indicates the methods of material preparation and initial separation of sterol subclasses from the extracted lipid.

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References

  1. Folch, J., Lees, M. and Stanley, G.H.S. J. Biol Chem., 226, 497 (1957).

    CAS  Google Scholar 

  2. Bligh, W.G. and Dyer, W.J. Can. J. Biochem. Physiol, 37, 911 (1959).

    CAS  Google Scholar 

  3. Macmurray, T.A. and Morrison, W.R. J. Sci. Food Agric., 21, 521 (1970)

    Google Scholar 

  4. Knights, B.A. Lipids, 6, 215 (1971).

    CAS  Google Scholar 

  5. Sobus, M.T. and Holmlund, C.E. Lipids, 11, 341 (1976).

    CAS  Google Scholar 

  6. Fishwick, M.J. and Wright, A.J. Phytochemistry, 16, 1507 (1977).

    CAS  Google Scholar 

  7. Nes, W.D., Saunders, G.A. and Heftmann, E. Lipids, 16, 744 (1981).

    CAS  Google Scholar 

  8. Osagie, A.U. and Kates, M. Lipids, 19, 958 (1984).

    CAS  Google Scholar 

  9. Johansson, A. and Appelqvist, L.-A. Lipids, 13, 658 (1978).

    CAS  Google Scholar 

  10. Westerman, L. and Roddick, L.G. Plant Physiol, 68, 872 (1981).

    CAS  Google Scholar 

  11. Knights, B.A. Lipids, 17, 204 (1982).

    CAS  Google Scholar 

  12. Nakanishi, S., Nishino, T., Yabusaki, Y., Fujisaki, S. and Katsuki, H. J. Biochem. (Tokyo), 96, 1665 (1984).

    CAS  Google Scholar 

  13. Harvey, H.R., Bradshaw, S.A., O’Hara, S.C.M., Eglinton, G. and Corner, E.D.S. Phytochemistry, 27, 1723 (1988).

    CAS  Google Scholar 

  14. Heupel, R.C. In Analysis of Sterols and Other Biologically Significant Steroids (W.D. Nes and E.J. Parish, eds), Chapter 1, pp. 1–3. Academic Press, New York (1989).

    Google Scholar 

  15. De Simone, F., Senatore, F., Sica, D. and Zollo, F. Phytochemistry, 18, 1572 (1979).

    Google Scholar 

  16. Fattorusso, E., Magno, S. and Mayol, L. Experientia, 36, 1137 (1980).

    CAS  Google Scholar 

  17. De Napoli, L., Magno, S., Mayol, L. and Novellino, E. Phytochemistry, 21, 1993 (1982).

    Google Scholar 

  18. Achari, B., Basu, K., Saha, C.R. and Pakrashi, S.C. Planta Medica, 40, 329 (1986).

    Google Scholar 

  19. Kikuchi, T., Kadota, S., Suehara, H. and Shima, T. Chem. Pharm. Bull, 33, 1914 (1985).

    CAS  Google Scholar 

  20. Erdman, T.R. and Thompson, R.H. Tetrahedron, 28, 5163 (1972).

    Google Scholar 

  21. Minale, L. and Sodano, G. J. Chem. Soc., Perkin Trans. 1, 1888 (1974).

    Google Scholar 

  22. Fattorusso, E., Magno, S., Mayol, L., Santacroce, C. and Sica, D. Tetrahedron, 31, 1715 (1975).

    CAS  Google Scholar 

  23. Steiner, E., Djerassi, C., Fattorusso, E., Magno, S., Mayol, L., Santacroce, C. and Sica, D. Helv. Chim. Acta, 60, 475 (1977).

    CAS  Google Scholar 

  24. Weete, J.D., Furter, R., Haeseler, E. and Rast, D.M. Can. J. Microbiol, 31, 1120 (1985).

    CAS  Google Scholar 

  25. Parks, L.W., Rodriguez, R.J. and Low, C. Lipids, 21, 89 (1986).

    CAS  Google Scholar 

  26. Weete, J.D. and Kelley, W.D. Lipids, 12, 398 (1977).

    CAS  Google Scholar 

  27. Renaud, R.L., Subden, R.E., Pierce, A.M. and Oehlschlager, A.C. Lipids, 13, 56 (1978).

    CAS  Google Scholar 

  28. Thompson, M.J., Dutky, S.R., Lehner, Y., Standifier, L.N. and Herbert, E.W. Jr. Phytochemistry, 17, 1053 (1978).

    CAS  Google Scholar 

  29. Warner, S.A., Sovocool, G.W. and Domnas, A.J. Mycologia, 75, 285 (1983).

    CAS  Google Scholar 

  30. Johansson, A. and Appelqvist, L.-A. Lipids, 13, 658 (1978).

    CAS  Google Scholar 

  31. Majumder, P.L. and Chakraborti, J. Tetrahedron, 41, 4973 (1985).

    CAS  Google Scholar 

  32. Majumder, P.L. and Pal, S. Phytochemistry, 29, 2717 (1990).

    CAS  Google Scholar 

  33. Beastall, G.H., Tyndall, A.M., Rees, H.H. and Goodwin, T.W. Eur. J. Biochem., 41, 301 (1974).

    CAS  Google Scholar 

  34. Loriaux, I.C., Morisaki, M. and Ikekawa, N. Phytochemistry, 15, 723 (1976).

    Google Scholar 

  35. Tanaka, Y., Tohara, K., Terasawa, K., Sawada, M. and Ageta, H. Shoyakugaku Zasshi, 32, 260 (1978).

    CAS  Google Scholar 

  36. Murakami, T., Tanaka, N., Satake, T., Saiki, Y. and Chen, C.-M. Yakugaku Zasshi, 105, 655 (1985).

    CAS  Google Scholar 

  37. Kokke, W.C.M.C., Bohlin, L., Fenical, W. and Djerassi, C. Phytochemistry, 21, 881 (1982).

    CAS  Google Scholar 

  38. Raederstorff, D. and Rohmer, M. Phytochemistry, 23, 2835 (1984).

    CAS  Google Scholar 

  39. Safe, S. Biochim. Biophys. Acta, 326, 471 (1973).

    CAS  Google Scholar 

  40. Palmer, M.A. and Bowden, B.N. Phytochemistry, 14, 1813 (1975).

    CAS  Google Scholar 

  41. Douglas, T.J. and Paleg, L.G. Phytochemistry, 17, 705 (1978).

    CAS  Google Scholar 

  42. Fayrett, J., Lacoste, L., Alais, J., Combier, A.L., Maquestiau, A., Van Harverbeke, Y., Flammang, R. and Mispreuve, H. Phytochemistry, 15, 49 (1976).

    Google Scholar 

  43. Ulbelen, A. Planta Medica, 30, 221 (1976).

    Google Scholar 

  44. Rauwald, H.-W., Sauter, M. and Schilcher, H. Phytochemistry, 24, 2746 (1985).

    CAS  Google Scholar 

  45. Lusby, W.R., Oliver, J.E., Mibben, G.H. and Thompson, M.J. Lipids, 22, 80 (1987).

    CAS  Google Scholar 

  46. Anding, C. and Ourisson, G. Eur. J. Biochem., 34, 345 (1973).

    CAS  Google Scholar 

  47. Volkman, J.K., Smith, D.J., Eglinton, G., Forsberg, T.E.V. and Corner, E.D.S. J. Mar. Biol Ass. U.K., 61, 509 (1981).

    CAS  Google Scholar 

  48. Kokke, W.C.M.C., Fenical, W. and Djerassi, C. Phytochemistry, 20, 127 (1981).

    CAS  Google Scholar 

  49. Ballantine, J.A., Lavis, A. and Morris, F.J. J. Exp. Mar. Biol Ecol, 53, 89 (1981).

    CAS  Google Scholar 

  50. Adams, B.G. and Parks, L.W. J. Lipid Res., 9, 8 (1968).

    CAS  Google Scholar 

  51. Ripperger, H. and Huneck, S. Phytochemistry, 15, 435 (1976).

    CAS  Google Scholar 

  52. Rohmer, M., Ourisson, G. and Brandt, R.D. Eur. J. Biochem., 31, 172 (1972).

    CAS  Google Scholar 

  53. Palmer, M.A. and Bowden, B.N. Phytochemistry, 14, 2049 (1975).

    CAS  Google Scholar 

  54. Heupel, R.C. Phytochemistry, 24, 2929 (1985).

    CAS  Google Scholar 

  55. Lozano, R., Chitwood, DJ., Lusby, W.R., Thompson, M.J., Svoboda, J.A. and Patterson, G.W. Comp. Biochem. Physiol., 79C, 21 (1984).

    CAS  Google Scholar 

  56. Chitwood, D.J., Hutzell, P.A. and Lusby, W.R. J. Nematology, 17, 64 (1985).

    CAS  Google Scholar 

  57. Salt, T.A., Lozano, R., Lusby, W.R., Chitwood, D.J. and Thompson, M.J. Steroids, 48, 451 (1986).

    CAS  Google Scholar 

  58. Chitwood, D.J., Lusby, W.R. and Salt, T.A. Comp. Biochem. Physiol., 86B, 103 (1987).

    CAS  Google Scholar 

  59. Gordon, D.T. J. Amer. Oil Chem. Soc., 59, 536 (1982).

    CAS  Google Scholar 

  60. Nichols, P.D. and Johns, R.B. Comp. Biochem. Physiol., 75B, 617 (1983).

    CAS  Google Scholar 

  61. Svoboda, J.A., Herbert, E.W. Jr, Thompson, M.J. and Feldlaufer, M.F. Lipids, 21, 97 (1986).

    CAS  Google Scholar 

  62. Csallany, A.S., Kindom, S.E., Addis, P.B. and Lee, J.-H. Lipids, 24, 645 (1989).

    CAS  Google Scholar 

  63. Hashimoto, F. and Hayashi, H. Biochim. Biophys. Acta, 1086, 115 (1991).

    CAS  Google Scholar 

  64. Li, L.N., Sjöstrand, U. and Djerassi, C. J. Amer. Chem. Soc., 103, 115 (1981).

    CAS  Google Scholar 

  65. Crist, B.V., Li, X., Bergquist, P.R. and Djerassi, C. J. Org. Chem., 48, 4472 (1983).

    CAS  Google Scholar 

  66. ApSimon, J.W. and Burnell, D.J. Comp. Biochem. Physiol., 65B, 567 (1980).

    CAS  Google Scholar 

  67. Teshima, S., Patterson, G.W. and Dutky, S.R. Lipids, 15, 1004 (1980).

    CAS  Google Scholar 

  68. Itoh, T. and Kaneko, H. Yukagaku, 23, 350 (1974).

    CAS  Google Scholar 

  69. Volkman, J.K., Gagosian, R.B. and Wakeham, S.G. Lipids, 19, 457 (1984).

    CAS  Google Scholar 

  70. Marlowe, I.T., Green, J.C., Neal, A.C., Brassell, S.C., Eglinton, G. and Course, P.A. Br. Phycol. J., 19, 203 (1984).

    Google Scholar 

  71. Harvey, H.R., Bradshaw, R.A., O’Hara, S.C.M., Eglinton, G. and Corner, E.D.S. Phytochemistry, 27, 1723 (1988).

    CAS  Google Scholar 

  72. Orcutt, D.M. and Richardson, B. Steroids, 16, 429 (1970).

    CAS  Google Scholar 

  73. Prahl, F.G., Eglinton, G., Corner, E.D.S., O’Hara, S.C.M. and Forsberg, T.E.V. J. Mar. Biol. Ass. U.K., 64, 317 (1984).

    CAS  Google Scholar 

  74. Takaishi, Y., Ohashi, T. and Tomimatsu, T. Phytochemistry, 28, 945 (1989).

    CAS  Google Scholar 

  75. Nes, W.R., Krevitz, K. and Behzadan, S. Lipids, 11, 118 (1976).

    CAS  Google Scholar 

  76. Kesselmeier, J., Eichenberger, W. and Urban, B. Plant Cell Physiol., 26, 463 (1985).

    CAS  Google Scholar 

  77. Kobayashi, M. and Mitsuhashi, H. Steroids, 26, 605 (1975).

    CAS  Google Scholar 

  78. Gupta, K.C. and Scheuer, P.J. Tetrahedron, 24, 5831 (1968).

    CAS  Google Scholar 

  79. Findlay, J.A. and Patil, A.D. Phytochemistry, 24, 366 (1985).

    CAS  Google Scholar 

  80. Boonyaratavej, S., Bates, R.B., Caldera, S. and Suvannachut, K. J. Nat. Prod., 53, 209 (1990).

    CAS  Google Scholar 

  81. Westerman, L. and Roddick, J.G. Plant Physiol., 68, 872 (1981).

    CAS  Google Scholar 

  82. Khan, A.Q., Ahmad, Z., Kazmi, S.N.-ul-H. and Malik, A. J. Nat. Prod. 51, 925 (1988).

    CAS  Google Scholar 

  83. Sheikh, Y.M. and Djerassi, C. Tetrahedron, 30, 4095 (1974).

    CAS  Google Scholar 

  84. Kobayashi, M., Kanda, F., Rao, C.L., Kumar, S.M.D., Trimurtulu, G. and Rao, C.B. Chem. Pharm. Bull., 38, 1724 (1990).

    CAS  Google Scholar 

  85. Serrazanetti, G.P., Cattani, O. and Piretti, M.V. Comp. Biochem. Physiol., 73B, 757 (1982).

    CAS  Google Scholar 

  86. Haughan, P.A., Lenton, J.R. and Goad, L.J. Phytochemistry, 27, 2491 (1988).

    CAS  Google Scholar 

  87. Seldes, A.M., Gros, E.G., Suarez, A., Rovirosa, J. and San-Martin, A. Tetrahedron, 44, 1359 (1988).

    CAS  Google Scholar 

  88. Kobayashi, M., Kawazoe, K., Katori, T. and Kitagawa, I. Chem. Pharm. Bull., 40, 1773 (1992).

    CAS  Google Scholar 

  89. Zielinski, J., Kokke, W.C.M.C., Tam Ha, T.B., Shu, A.Y.L., Duax, W.L. and Djerassi, C, J. Org. Chem., 48, 3471 (1983).

    CAS  Google Scholar 

  90. Catalan, C.A.N., Thompson, J.E., Kokke, W.C.M.C. and Djerassi, C. Tetrahedron, 41, 1073 (1985).

    CAS  Google Scholar 

  91. Avruch, L., Fischer, S., Pierce, H. Jr and Oehlschlager, A.C. Can. J. Biochem., 54, 657 (1976).

    CAS  Google Scholar 

  92. Withers, N.W., Tuttle, R.C., Holz, G.G., Beach, D.H., Goad, L.J. and Goodwin, T.W. Phytochemistry, 17, 1987 (1978).

    CAS  Google Scholar 

  93. Nichols, P.D., Volkman, J.K. and Johns, R.B. Phytochemistry, 22, 1447 (1983).

    CAS  Google Scholar 

  94. Nes, W.R., Dhanuka, I.C. and Pinto, W.J. Lipids, 21, 102 (1986).

    CAS  Google Scholar 

  95. Kren, V., Rezanka, T., Sajdl, P. and Rehacek, Z. Biochem. Physiol Pflanzen, 181, 505 (1986).

    CAS  Google Scholar 

  96. Smith, A.G., Rubinstein, I. and Goad, L.J. Biochem. J., 135, 443 (1973).

    CAS  Google Scholar 

  97. Teshima, S., Kanazawa, A., Hyodo, S. and Ando, T. Comp. Biochem. Physiol, 64B, 225 (1979).

    CAS  Google Scholar 

  98. Gibbons, G.F., Goad L.J. and Goodwin, T.W. Phytochemistry, 6, 677 (1967).

    Google Scholar 

  99. Popov, S., Carlson, R.M.K., Wegmann, A. and Djerassi, C. Steroids, 28, 699 (1976).

    CAS  Google Scholar 

  100. Knights, B.A. Phytochemistry, 9, 903 (1970).

    CAS  Google Scholar 

  101. van Lier, J.A. and Smith, L.L. Steroids, 15, 485 (1970).

    Google Scholar 

  102. Gladu, P.K., Patterson, G.W., Wikfors, G.H. and Lusby, W.R. Lipids, 26, 656 (1991).

    CAS  Google Scholar 

  103. Nes, W.D. and Heupel, R.C. Arch. Biochem. Biophys., 244, 211 (1986).

    CAS  Google Scholar 

  104. Lin, C.-N., Tome, W.-P. and Won, S.-J. Phytochemistry, 29, 673 (1990).

    CAS  Google Scholar 

  105. Popov, S.S., Marekov, N.L., Konaklieva, M.I., Panayotova, M.I. and Koraklieva, S.D. Phytochemistry, 24, 1987 (1985).

    CAS  Google Scholar 

  106. Cranwell, P.A., Jaworski, G.H.M. and Bickley, H.M. Phytochemistry, 29, 145 (1990).

    CAS  Google Scholar 

  107. Ekman, R. and Karunen, P. Phytochemistry, 19, 1243 (1980).

    CAS  Google Scholar 

  108. Tanaka, Y., Hiyama, R. and Ageta, H. Chem. Pharm. Bull, 38, 2130 (1990).

    Google Scholar 

  109. Kamaya, R., Mori, T., Shiji, K., Ageta, H., Chang, H.-C. and Hsu, H.-Y. Yakugaku Zasshi, 111, 120 (1991).

    CAS  Google Scholar 

  110. Kasprzyk, Z., Sliwowski, J. and Skawarko, B. Phytochemistry, 11, 1961 (1972).

    CAS  Google Scholar 

  111. Staphylakis, K. and Gegiou, D. Lipids, 20, 723 (1985).

    CAS  Google Scholar 

  112. Heupel, R.C., Sauvaire, Y., Le, P.H., Parish, E.J. and Nes, W.D. Lipids, 21, 69 (1986).

    CAS  Google Scholar 

  113. Noda, M., Tanaka, M., Seto, Y., Aiba, T. and Oku, C. Lipids, 23, 439 (1988).

    CAS  Google Scholar 

  114. Kalinowska, M., Nes, W.R., Crumley, F.G. and Nes, W.D. Phytochemistry, 29, 3427 (1990).

    CAS  Google Scholar 

  115. Dyas, L., Threlfall, D.R. and Goad, L.J. Phytochemistry, 35, 655 (1994).

    CAS  Google Scholar 

  116. Leitao, S.G., Kaplan, M.A.C., Monache, F.D., Akihisa, T. and Tamura, T. Phytochemistry, 31, 2813 (1992).

    CAS  Google Scholar 

  117. Leitao, S.G., Kaplan, M.A.C. and Monache, F.D. Phytochemistry, 36, 167 (1994).

    CAS  Google Scholar 

  118. Salt, T.A., Chitwood, D.J. and Lusby, W.R. Lipids, 24, 325 (1989).

    CAS  Google Scholar 

  119. Sheffer, M., Fried, A., Gottlieb, H.E., Tietz, A. and Avron, M. Biochim. Biophys. Acta, 857, 165 (1986).

    CAS  Google Scholar 

  120. Parks, L.W., Bond, F.T., Thompson, E.D. and Starr, P.R. J. Lipid Res., 13, 311 (1972).

    CAS  Google Scholar 

  121. Kohlhase, M. and Pohl, P. Phytochemistry, 21, 1735 (1988).

    Google Scholar 

  122. Conner, A.H., Nagasampagi, B.A. and Rowe, J.W. Phytochemistry, 19, 1121 (1980).

    CAS  Google Scholar 

  123. Kabore, S.A., Combaut, G., Vidal, J.-P., Codomier, L., Passet, J., Girard, J.-P. and Rossi, J.-C. Phytochemistry, 22, 1239 (1983).

    CAS  Google Scholar 

  124. Ando, T., Ferezou, J.-P. and Barbier, M. Biochem. Syst. Ecol, 3, 247 (1975).

    CAS  Google Scholar 

  125. Evans, F.J. J. Pharm. Pharmacol, 25, 156 (1973).

    CAS  Google Scholar 

  126. Yokokawa, H. Phytochemistry, 19, 2615 (1980).

    CAS  Google Scholar 

  127. Yokokawa, H. and Mitsuhashi, T. Phytochemistry, 20, 1349 (1981).

    CAS  Google Scholar 

  128. Endo, S., Abe, M., Ayabe, K., Kokubo, T., Yamamoto, S. and Mitsuhashi, T. Bull. Tokyo Gakugei Univ., Ser. IV 332, 91 (1980).

    Google Scholar 

  129. Pollio, A., Greca, M.D., Monaco, P., Pinto, G. and Previtera, L. Biochim. Biophys. Acta, 963, 53 (1988).

    CAS  Google Scholar 

  130. Theobald, N., Wells, R.J. and Djerassi, C. J. Amer. Chem. Soc., 100, 7677 (1978).

    CAS  Google Scholar 

  131. Eggersdorfer, M.L., Kokke, W.C.M.C., Crandell, C.W., Hochlowski, J.E. and Djerassi, C. J. Org. Chem ., 47, 5304 (1982).

    CAS  Google Scholar 

  132. Xu, S., Norton, R.A., Crumley, F.G. and Nes, W.D. J. Chromatogr., 452, 377 (1988).

    CAS  Google Scholar 

  133. Bianchini, J.-P., Gaydou, E.M., Sigoillot, J.-C. and Terrom, C. J. Chromatogr., 329, 231 (1985).

    CAS  Google Scholar 

  134. Farines, M., Cocallemen, S. and Soulier, J. Lipids, 23, 349 (1988).

    CAS  Google Scholar 

  135. Zygadlo, J.A. Phytochemistry, 35, 163 (1994).

    CAS  Google Scholar 

  136. Johansson, A. Lipids, 14, 285 (1979).

    CAS  Google Scholar 

  137. Saranpaa, P. and Nyberg, H. Trees, 1, 82 (1987).

    CAS  Google Scholar 

  138. Pillai, C.G.P. and Weete, J.D. Phytochemistry, 14, 2347 (1975).

    CAS  Google Scholar 

  139. Svoboda, J.A., Rees, H.H., Thompson, M.J. and Hoggard, N. Steroids, 53, 329 (1989).

    CAS  Google Scholar 

  140. Goad, L.J., Holz, G.G. Jr. and Beach, D.H. Phytochemistry, 22, 475 (1983).

    CAS  Google Scholar 

  141. Weete, J.D., Fulifaj, M., Montani, C., Nes, W.R. and Sancholle, M. Can. J. Microbiol., 31, 1127 (1985).

    CAS  Google Scholar 

  142. Thompson, M.J., Patterson, G.W., Dutky, S.R., Svoboda, J.A. and Kaplanis, J.N. Lipids, 15, 719 (1980).

    CAS  Google Scholar 

  143. Davis, N.D., Cole, R.J., Dorner, J.W., Weete, J.D., Backman, P.A., Clark, E.M., King, C.C., Schmidt, S.P. and Diener, U.L. J Agric. Food Chem., 34, 105 (1986).

    CAS  Google Scholar 

  144. Weete, J.D., Fuller, M.S., Huang, M.Q. and Gandhi, S. Exp. Mycol., 13, 183 (1989).

    CAS  Google Scholar 

  145. Chiu, P.-L., Patterson, G.W. and Salt, T.A. Phytochemistry, 27, 819 (1988).

    CAS  Google Scholar 

  146. Maia, G.A., Brown, W.H., Whiting, F.M. and Stull, J.W. J. Food Sci., 41, 190 (1976).

    CAS  Google Scholar 

  147. Hanny, B.W. and Gueldner, R.C. J. Agric. Food Chem., 24, 401 (1976).

    CAS  Google Scholar 

  148. Russell, G.B., Connor, H.E. and Purdie, A.W. Phytochemistry, 15, 1933 (1976).

    CAS  Google Scholar 

  149. Smith, R.M. and Martin-Smith, M. Phytochemistry, 17, 1307 (1978).

    CAS  Google Scholar 

  150. Stevens, J.F., Hart, H., Block, A., Zwaving, J.H. and Malingre, T.M. Phytochemistry, 35, 389 (1994).

    CAS  Google Scholar 

  151. Stevens, J.F., Hart, H.T., Pouw, A.J.A., Block, A. and Zwaving, J.H. Phytochemistry, 36, 341 (1994).

    CAS  Google Scholar 

  152. Safe, L.M., Wong, C.J. and Chandler, R.F. J. Pharm. Sci., 63, 464 (1974).

    CAS  Google Scholar 

  153. Adler, G. and Kasprzyk, Z. Phytochemistry, 14, 627 (1975).

    CAS  Google Scholar 

  154. Bergquist, R.P., Lavis, A. and Cambie, R.C. Biochem. Syst. Ecol, 14, 105 (1986).

    CAS  Google Scholar 

  155. Gonzalez, R.A. and Parks, L.W. Biochim. Biophys. Acta, 489, 507 (1977).

    Google Scholar 

  156. Rodriguez, R.J. and Parks, L.W. Anal. Biochem., 119, 200 (1982).

    CAS  Google Scholar 

  157. Rodriguez, R.J. and Parks, L.W. Arch. Biochem. Biophys., 225, 861 (1983).

    CAS  Google Scholar 

  158. Knapp, F.F., Phillips, D.O., Goad, L.J. and Goodwin, T.W. Phytochemistry, 11, 3497 (1972).

    CAS  Google Scholar 

  159. Bouvier, P., Rohmer, M., Benveniste, P. and Ourisson, G. Biochem. J., 159, 267 (1976).

    CAS  Google Scholar 

  160. Gladu, P.K., Patterson, G.W., Wikfors, G.H., Chitwood, D.J. and Lusby, W.R. Comp. Biochem. Physiol., 97B, 491 (1990).

    CAS  Google Scholar 

  161. Alam, M., Sansing, T.B., Guerra, J.R. and Harmon, A.D. Steroids, 38, 2807 (1981).

    Google Scholar 

  162. Wengrovitz, P.S., Sanduja, R. and Alam, M. Comp. Biochem. Physiol. 59B, 535 (1981).

    Google Scholar 

  163. Goad, L.J. and Withers, N.W. Lipids, 17, 853 (1982).

    CAS  Google Scholar 

  164. Alam, M., Sanduja, R., Watson, D.A. and Laeblich, A.R. III. J. Phycol., 20, 331 (1984).

    CAS  Google Scholar 

  165. Nichols, P.D., Volkman, J.K., Hallegraeff, G.M. and Blackburn, S.I. Phytochemistry, 26, 2537 (1987).

    CAS  Google Scholar 

  166. Combaut, C., Codomier, L., Teste, J. and Pedersen, M. Phytochemistry, 20, 1748 (1981).

    CAS  Google Scholar 

  167. Lisboa, B.P., Ganshow, I., Halket, J.M. and Joventino, F.P. Comp. Biochem. Physiol., 73B, 257 (1982).

    CAS  Google Scholar 

  168. Ziogas, B.Z., Sisler, H.D. and Lusby, W.R. Pestic. Biochem. Physiol., 20, 320 (1983).

    CAS  Google Scholar 

  169. Jain, A.C. and Guppta, S.K. Phytochemistry, 23, 2392 (1984).

    CAS  Google Scholar 

  170. Herber, R., Villoutreix, J., Granger, P. and Chapelle, S. Can. J. Microbiol., 29, 606 (1983).

    CAS  Google Scholar 

  171. Norton, R.A. and Nes, W.D. Lipids, 26, 247 (1991).

    CAS  Google Scholar 

  172. Nes, W.D., Janssen, G.G., Crumley, F.G., Kalinowska, M. and Akihisa, T. Arch. Biochem. Biophys., 300, 724 (1993).

    CAS  Google Scholar 

  173. Adler, J.H. Phytochemistry, 22, 607 (1983).

    CAS  Google Scholar 

  174. Chiu, P.-L., Patterson, G.W. and Fenner, G.P. Phytochemistry, 24, 263 (1985).

    CAS  Google Scholar 

  175. Laonigro, G., Adinolfi, M., Barone, G., Lanzetta, R. and Mangoni, L. Gazz. Chim. Ital., 112, 273 (1982).

    CAS  Google Scholar 

  176. Dyas, L., Prescott, M.C., Evershed, R.P. and Goad, L.J. Lipids, 26, 536 (1991).

    CAS  Google Scholar 

  177. Rahier, A., Taton, M., Schmitt, P., Benveniste, P., Place, P. and Anding, C. Phytochemistry, 24, 1223 (1985).

    CAS  Google Scholar 

  178. Heupel, R.C. and Nes, W.D. Phytochemistry, 24, 2905 (1985).

    CAS  Google Scholar 

  179. Misso, N.L.A. and Goad, L.J. Phytochemistry, 23, 72 (1984).

    Google Scholar 

  180. Itoh, T., Sica, D. and Djerassi, C. J. Chem. Soc., Perkin Trans. 1, 147 (1983).

    Google Scholar 

  181. Kitagawa, I., Kobayashi, M., Kitanaka, K., Kido, M. and Kyogoku, Y. Chem. Pharm. Bull., 31, 2321 (1983).

    CAS  Google Scholar 

  182. de Riccardis, F., Minale, L., Iorizzi, M., Debitus, C. and Levi, C. J. Nat. Prod., 56, 282 (1983).

    Google Scholar 

  183. Bohlin, L., Sjoestrand, U. and Djerassi, C. J. Chem. Soc., Perkin Trans. 1, 1023 (1981).

    Google Scholar 

  184. Miralles, J., Diop, M., Ferrer, A. and Kornprobst, J.-M. Comp. Biochem. Physiol., 89B, 209 (1988).

    CAS  Google Scholar 

  185. Popov, S., Soilov, I., Marekov, N., Kovachev, G. and Andreev, St. Lipids, 16, 663 (1981).

    CAS  Google Scholar 

  186. Piretti, M.V., Serrazanetti, G.P. and Viviani, R. Comp. Biochem. Physiol., 73B, 211 (1982).

    CAS  Google Scholar 

  187. Sato, S., Ikekawa, N., Kanazawa, A. and Ando, T. Steroids, 36, 2619 (1980).

    Google Scholar 

  188. Kalinovskaya, N.I., Kuznetsova, T.A., Popov, A.M., Antonov, S.A. and Elyakov, G.B. Comp. Biochem. Physiol., 76B, 167 (1983).

    CAS  Google Scholar 

  189. Matsuoka, C., Nohta, H., Kuroda, N. and Ohkura, Y. J. Chromatogr., 341, 432 (1985).

    CAS  Google Scholar 

  190. Devgun, M.S. and Paterson, C.R. J Chromatogr., 353, 169 (1986).

    CAS  Google Scholar 

  191. Hara, A. and Radin, N.S. Anal. Biochem., 90, 420 (1978).

    CAS  Google Scholar 

  192. Zielinski, J., Milkova, T., Popov, S., Marekov N. and Djerassi, C. Steroids, 39, 675 (1982).

    CAS  Google Scholar 

  193. Tam Ha, T.B., Kokke, W.C.M.C. and Djerassi, C. Steroids, 40, 433 (1982).

    Google Scholar 

  194. Akihisa, T., Ghosh, P., Thakur, S., Nagata, H., Tamura T. and Matsumoto, T. Phytochemistry, 29, 1639 (1990).

    CAS  Google Scholar 

  195. Kennedy, J.A. and Brassell, S.C. Org. Geochem., 19, 235 (1992).

    CAS  Google Scholar 

  196. Santos, V., Billett, D.S.M., Rice A.L. and Wolff, G.A. Deep-Sea Res. I, 41, 787 (1994).

    Google Scholar 

  197. Wakeham, S.G. and Canuel, E.A. Org. Geochem., 16, 703 (1990).

    CAS  Google Scholar 

  198. Wakeham, S.G. and Canuel, E.A. J. Marine Res., 46, 182 (1988).

    Google Scholar 

  199. Robinson, N., Eglinton, G., Bessell, S.C. and Cranwell, P.A. Nature, 308, 439 (1984).

    CAS  Google Scholar 

  200. Lenton, J.R., Goad, L.J. and Goodwin, T.W. Phytochemistry, 12, 1135 (1973).

    CAS  Google Scholar 

  201. Brandt, R.D., Pryce, R.J., Anding, C. and Ourisson, G. Eur. J. Biochem., 17, 344 (1970).

    CAS  Google Scholar 

  202. Rifai, N. and Warnick, G.R. Laboratory Measurement of Lipids, Lipoproteins and Apolipoproteins. AACC Press, Washington DC, USA (1995).

    Google Scholar 

  203. Cranwell, P.A. Progr. Lipid Res., 21, 271 (1982).

    CAS  Google Scholar 

  204. Maduriera, L.A.S., Eglinton, G. and Conte, M. In Organic Geochemistry — advances and applications in the natural environment (D.A. Manning, ed.), p. 243, Manchester University Press, Manchester (1991).

    Google Scholar 

  205. Saliot, A., Laureillard, J., Scribe, P. and Sicre, M.A. Marine Chem., 36, 233 (1991).

    CAS  Google Scholar 

  206. Volkman, J.K. Org. Geochem., 9, 83 (1981)

    Google Scholar 

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Authors and Affiliations

  1. School of Biological Sciences, University of Liverpool, UK

    L. John Goad

  2. College of Science and Technology, Nihon University, Tokyo, Japan

    Toshihiro Akihisa

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Goad, L.J., Akihisa, T. (1997). Extraction of sterols from tissues. In: Analysis of Sterols. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1447-6_2

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