Abstract
A new purification procedure for purification of Pop Corn Inhibitor, the trypsin and activated factor XII inhibitor present in corn, is presented. It is shown that Polenta is a more convenient starting material than the more commonly used sweet corn kernels. Complete purification of the inhibitor is obtained through use of one chromatography step on Procion red HE-3B immobilized on agarose followed by a reverse phase chromatography step. Advantages of this procedure compared to those using immobilized trypsin or immobilized anhydro trypsin are discussed.
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Swartz, M.J., Mitchell, H.L., Cox, D.J., Reeck, G.R., Isolation and characterization of trypsin inhibitor from opaque-2 corn seeds. J Biol. Chem. 1977, 252, 8105–7.
Hojima, Y., Pierce, J.V. and Pisano, J.J., Hageman factor fragment inhibitor in corn seeds: purification and characterization. Thromb. Res. 1980, 20, 149–62.
G. Tans, T. Jansseen-Claessen, J. Rossing and Griffin J.H., Studies on the effect of serine protease inhibitors on activated contact factors. Application in amidolytic assays for factor XIIa, Plasma kallikrein and Factor XIa. Eur. J. Biochem. 1987, 164, 637–42.
Lei, M.G., and Reek, G.R., Combined use of trypsin agarose affinity chromatography and reverse phase chromatography and reversed phase high performance liquid chromatography for the purification of single chain protease inhibitor from corn seeds. J. Chromatogr. 1986, 363, 315–21.
Mahoney, W.C., Hermodson M.A., Jones, B., Powers, D.D., Corfman, R.S. and Reek, G.R., Amino acid sequence and secondary structural analysis of the corn trypsin inhibitoor of trypsin and and activated Hageman factor. J. Biol. Chem. 1984, 259, 8412–6.
Algiman, E. Bertrand, O., Unpublished results.
Yokosawa, H. and Ishii, S.I., Anhydrotrypsin: new features in ligand interactions revealed by affinity chromatography and thionine replacement. J. Biochem. 1977, 81, 647–56.
Kutty, A.V.M. and Pattabiraman, T.N., Behavior of plant amylase and trypsin inhibitors during dye-ligand chromatography on blue sepharose and red sepharose. Biochem. Arch. 1987, 3, 231–40.
Kroviarski, Y., Cochet, S., Vadon, C., Truskolaski, A., Boivin, P., Bertrand, O., New strategies for the screening of a large number of immobilized dyes for purification of enzymes. Application to purification from human hemolyzate. J. Chromatogr. (in press).
Qadri, F. and Dean, P.D.G, The use of various immobilized triazine affinity dyes for the purification of 6-phospho gluconate dehydrogenase from Bacillus Stearothermophilus. Biochem. J. 1980, 191 53–62.
Bradford, M.M, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of Protein dye binding. Anal. Biochem. 1976, 72, 248–54.
Scopes, R.K., Strategies for enzyme isolation using dye-ligand and related adsorbents. J. Chromatogr., 1986, 376, 131–40.
Halim, A.H., Wassom, C.E. and Mitchell, H.L., Trypsin Inhibitor in Corn (Zea mays L.) as influenced by genotype and moisture stress. Crop Science 1973, 13, 405–7.
Bertrand, O., Cochet, S., Kroviarski, Y., Truskolaski, A. and Boivin, P., A low cost modular apparatus of large applicability for automation of chromatographies. Proteins purification technologies ed. A. Faure GRBP Paris 1986, pp. 205–7.
Kassel, B., Naturally occuring Inhibitors of Proteolytic enzymes In Methods in Enzymology Proteolytic Enzymes part A ed. G. Perlmann and L. Lorand. Academic Press New York 1970 pp. 839–906.
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Algiman, E., Kroviarski, Y., Cochet, S., Truskolaski, A., Boivin, P., Bertrand, O. (1989). A New Procedure for Purification of Pop Corn Inhibitor Based on Immobilized Dye Chromatography. In: Vijayalakshmi, M.A., Bertrand, O. (eds) Protein-Dye Interactions: Developments and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1107-9_30
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DOI: https://doi.org/10.1007/978-94-009-1107-9_30
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6989-2
Online ISBN: 978-94-009-1107-9
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