Skip to main content
SpringerLink
Log in

Pseudomonas aeruginosa immunization

  • Chapter
Recent Developments in Prophylactic Immunization

Part of the book series: Immunology and Medicine ((IMME,volume 12))

  • 42 Accesses

Abstract

Pseudomonas aeruginosa, formerly known as Bacillus pyocyaneus, was described over 100 years ago by Gessard1. However, as recently as 1947 this ubiquitous environmental inhabitant was described as rarely pathogenic for man2. The advent of modern medical practices, however, including antimicrobial agents, immuno- and myelosuppressive chemotherapy and sophisticated life-support equipment, has produced a population of patients at increased risk for infection with opportunistic infectious agents. P. aeruginosa has emerged as one of the most common and most problematic among these modern opportunistic pathogens3,4. Perhaps of most concern has been the unusually high mortality associated with P. aeruginosa bacteraemia and pneumonia, often twice that observed with other Gram-negative bacillary infections4–6.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gessard, C. (1882). Sur les colorations bleue et verte des linges à pansements. C.R. Sceances Acad. Sci. Serie D, 94, 536–8

    Google Scholar 

  2. Stanley, M. (1947). Bacillus pyocyaneus infections. A review, report of cases and discussion of newer therapy including streptomycin. Am. J. Med., 2, 253, 347–67

    Article  PubMed  CAS  Google Scholar 

  3. Reynolds, H. Y., Levine, A. S., Wood, R. E., Zierdt, C. H., Dale, D. C. and Pennington, J. E. (1975). Pseudomnas aeruginosa infections: persisting problems and current research to find new therapies. Ann. Intern. Med., 82, 819–31

    Google Scholar 

  4. Young, L. S., Wenzel, R. P., Sabath, L. D., Pollack, M, Pennington, J. E. and Platt, R. (1984). The outlook for prevention and treatment of infections due to Pseudomonas aeruginosa. Rev. Infect. Dis., 6 (Suppl. 3), S769–74

    Article  PubMed  Google Scholar 

  5. Stevens, R. M., Teres, D., Skillman, J. J. and Feingold, D. S. (1974). Pneumonia in an intensive care unit. Arch. Intern. Med., 134, 106–11

    Article  PubMed  CAS  Google Scholar 

  6. Bryan, C. S. and Reynolds, K. L. (1984). Bacteremic nosocomial pneumonia. Am. Rev. Resp. Dis., 129, 668–71

    PubMed  CAS  Google Scholar 

  7. Nicas, T. I. and Iglewski, B. H. (1985). The contribution of exoproducts to virulence of Pseudomonas aeruginosa. Can. J. Microbiol., 31, 387–92

    Article  PubMed  CAS  Google Scholar 

  8. Young, L. S. (1972). Human immunity to Pseudomonas aeruginosa. II. Relationship between heat-stable opsonins and type-specific lipopolysaccharides. J. Infect. Dis., 126, 277–87

    Article  PubMed  CAS  Google Scholar 

  9. Reynolds, H. Y., Kazmierowski, J. A. and Newball, H. H. (1975). Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages. J. Clin. Invest., 56, 376–85

    Article  PubMed  CAS  Google Scholar 

  10. Bjornson, A. B. and Michael, J. G. (1972). Contribution of humoral and cellular factors to the resistance to experimental infection by Pseudomonas aeruginosa in mice. II. Opsonic, agglutinative, and protective capacities of immunoglobulin G anti-Pseudomonas antibodies. Infect. Immun., 5, 775–82

    PubMed  CAS  Google Scholar 

  11. Groves, E. H. (1909). Case of bacillus pyocyaneus pyaemia successfully treated by vaccine. Br. Med. J., 1, 1169–70

    Article  PubMed  CAS  Google Scholar 

  12. Feller, I. and Pierson, C. (1968). Pseudomonas vaccine and hyperimmune plasma for burned patients. Arch. Surg., 97, 225–9

    PubMed  CAS  Google Scholar 

  13. Kefalides, N. A., Arana, J. A., Bazan, A., Boganegra, M., Stastny, P., Velarde, N. and Rosenthal, S. M. (1962). Role of infection in mortality from severe burns. N. Engl. J. Med., 267, 317–23

    Article  PubMed  CAS  Google Scholar 

  14. Bocanegra, M., Hinostroza, F., Bazan, A., Velarde, N., Yoza, V. and Rosenthal, S. M. (1966). Convalescent burn plasma therapy for severely burned children. Ann. Surg., 163, 461–9

    Article  PubMed  CAS  Google Scholar 

  15. Hanessian, S., Regan, W., Watson, D. and Haskel, T. H. (1971). Isolation and characterization of antigenic components of a new heptavalent Pseudomonas vaccine. Nature, 229, 209–10

    CAS  Google Scholar 

  16. Fisher, M. W., Devlin, H. B. and Gnabasik, F. J. (1969). New immunotype schema for Pseudomonas aeruginosa based on protective antigens. J. Bacteriol., 98, 835–6

    PubMed  CAS  Google Scholar 

  17. Young, L. S., Meyer, R. D. and Armstrong, D. (1973). Pseudomonas aeruginosa vaccine in cancer patients. Ann. Intern. Med., 79, 518–27

    PubMed  CAS  Google Scholar 

  18. Alexander, J. W. and Fisher, M. W. (1974). Immunization against Pseudomonas in infection after thermal injury. J. Infect. Dis., 130 (Suppl.), S152–8

    Article  Google Scholar 

  19. Polk, H. C., Borden, S. and Aldret, J. A. (1973). Prevention of Pseudomonas respiratory infection in a surgical intensive care unit. Arch. Surg., 177, 607–13

    Google Scholar 

  20. Pennington, J. E., Reynolds, H. Y., Wood, R. E., Robinson, R. A. and Levine, A. S. (1975). Use of Pseudomonas aeruginosa vaccine in patients with acute leukemia and cystic fibrosis. Am. J. Med., 58, 629–36

    Article  PubMed  CAS  Google Scholar 

  21. Miler, J. J., Spilsbury, J. F., Jones, R. J., Roe, E. A. and Lowbury, E. J. L. (1977). A new polyvalent Pseudomonas vaccine. J. Med. Microbiol., 10, 19–27

    Article  PubMed  CAS  Google Scholar 

  22. Jones, R. J., Roe, E. A., Lowbury, E. J. L., Miler, J. J. and Spilsbury, J. F. (1976). A new Pseudomonas vaccine: preliminary trial on human volunteers. J. Hyg. Comb., 76, 429–39

    Article  CAS  Google Scholar 

  23. Roe, E. A. and Jones, R. J. (1983). Immunization of burned patients against Pseudomonas aeruginosa infection at Safdarjang Hospital, New Delhi. Rev. Infect. Dis., 5 (Suppl.), 922–30

    Article  Google Scholar 

  24. Maclntyre, S., McVeigh, T. and Owen, P. (1986). Immunochemical and biochemical analysis of the polyvalent Pseudomonas aeruginosa vaccine PEV. Infect. Immun., 51, 675–86

    Google Scholar 

  25. Pier, G. B., Sidberry, H. F., Zolyomi, S. F. and Sadoff, J. C. (1978). Isolation and characterization of high molecular weight polysaccharide from the slime of Pseudomonas aeruginosa. Infect. Immun., 22, 908–18

    PubMed  CAS  Google Scholar 

  26. Pier, G. B. (1982). Safety and immunogenicity of high molecular weight polysaccharide vaccine from immunotype 1 Pseudomonas aeruginosa. J. Clin. Invest., 69, 303–8

    Article  PubMed  CAS  Google Scholar 

  27. Homma, J. Y. (1986). The protein moiety of the endotoxin of Pseudomonas aeruginosa. Z. Allg. Microbiol., 8, 227–48

    Google Scholar 

  28. Abe, C., Shionoya, H., Hirao, Y., Okada, K. and Homma, J. Y. (1975). Common protective antigen (OEP) of Pseudomonas aeruginosa. Jpn. J. Exp. Med., 45, 355–59

    PubMed  CAS  Google Scholar 

  29. Okada, K., Kawaharajo, K., Kasai, K. and Homma, J. Y. (1980). Effects of somatic components of Pseudomonas aeruginosa on protective immunity in experimental mouse burn infection. Jpn. J. Exp. Med., 50, 53–61

    PubMed  CAS  Google Scholar 

  30. Pollack, M. S. and Young, L. S. (1979). Protective activity of antibodies of exotoxin A and lipopolysaccharide at the onset of Pseudomonas aeruginosa septicemia in man. J. Clin. Invest., 63, 276–86

    Article  PubMed  CAS  Google Scholar 

  31. Pavlovskiy O. R., Edman, D. C., Leppla, S. H, Wretland, B., Lewis, L. R. and Martin, K. E. (1981). Protection against experimental Pseudomonas aeruginosa infection in mice by active immunization with exotoxin A toxoids. Infect. Immun., 32, 681–9

    Google Scholar 

  32. Cryz, S. J., Furer, E. and Germanier, R. (1983). Protection against Pseudomonas aeruginosa infection in a murine burn wound sepsis model by passive transfer of antitoxin A, antielastase, and antilipopolysaccharide. Infect. Immun., 39, 1072–9

    PubMed  CAS  Google Scholar 

  33. Cryz, S. J., Furer, E. and Germanier, R. (1983). Passive protection against Pseudomonas aeruginosa infection in an experimental leukopenic mouse model. Infect. Immun., 40, 659–64

    PubMed  Google Scholar 

  34. Woods, D. E., Bass, J. A., Johanson, W. G. Jr. and Straus, D. C. (1980). Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infect. Immun., 30, 694–9

    PubMed  CAS  Google Scholar 

  35. Woods, D. E., Straus, D. C., Johanson, W. G. Jr., Berry, V. K. and Bass, J. A. (1980). Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect. Immun., 29, 1146–51

    PubMed  CAS  Google Scholar 

  36. Montie, T. C., Doyle-Huntzinger, D., Craven, R. C. and Holder, I. A. (1982). Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model. Infect. Immun., 38, 1296–8

    PubMed  CAS  Google Scholar 

  37. Holder, I. A. and Naglich, J. G. (1986). Experimental studies of the pathogenesis of infections due to Pseudomnas aeruginosa immunization using divalent flagella preparations. J. Trauma, 26, 118–22

    Article  PubMed  CAS  Google Scholar 

  38. Holder, I. A., Wheeler, R. and Montie T. C. (1982). Flagellar preparations from Pseudomonas aeruginosa: animal protection studies. Infect. Immun., 35, 276–80

    PubMed  CAS  Google Scholar 

  39. Anderson, T. R. and Montie, T. C. (1987). Opsonophagocytosis of Pseudomonas aeruginosa treated with antiflagellar serum. Infect. Immun., 55, 3204–6

    PubMed  CAS  Google Scholar 

  40. Mutharia, L. M., Nicas, T. I. and Hancock, R. E. W. (1982). Outer membrane proteins of Pseudomonas aeruginosa serotype strains. J. Infect. Dis., 146, 770–9

    Article  PubMed  CAS  Google Scholar 

  41. Lam, J. S., Mutharia, L. M., Hancock, R. E. W., Hoiby, N., Lam, K., Baek, L. and Costerton, J. W. (1983). Immunogenicity of Pseudomonas aeruginosa outer membrane antigens examined by crossed Immunoelectrophoresis. Infect. Immun., 42, 88–98

    PubMed  CAS  Google Scholar 

  42. Mathews-Greer, J. M. and Gilleland, Jr. H. E. (1987). Outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine against heterologous immunotype strains in a burned mouse model. J. Infect. Dis., 155, 1282–91

    Article  Google Scholar 

  43. Sawada, S., Suzuki, M., Kawamura, T., Fujinaga, S., Masuho, Y. and Tomibe, K. (1984). Protection against infection with Pseudomonas aeruginosa by passive transfer of monoclonal antibodies to lipopolysaccharides and outer membrane proteins. J. Infect. Dis., 150, 570–6

    Article  PubMed  CAS  Google Scholar 

  44. Pennington, J. E., Small, G. J., Lostrom, M. E. and Pier, G. B. (1986). Polyclonal and monoclonal antibody therapy for experimental Pseudomonas aeruginosa pneumonia. Infect. Immun., 54, 239–44

    PubMed  CAS  Google Scholar 

  45. Pennington, J. E. and Pier, G. B. (1983). Efficacy of cell wall Pseudomonas aeruginosa vaccines for protection against experimental pneumonia. Rev. Infect. Dis., 5 (Suppl. 5), S852–7

    Article  PubMed  Google Scholar 

  46. Cryz, S. J., Jr, Fürer, E., Sadoff, J. C. and Germanier, R. (1986). Pseudomonas aeruginosa immunotype 5 polysaccharide-toxin A conjugate vaccine. Infect. Immun., 52, 161–5

    PubMed  CAS  Google Scholar 

  47. Cryz, S. J. Jr, Fürer, E., Cross, A. S., Wegmann, A., Germanier, R. and Sadoff, J. C. (1987). Safety and immunogenicity of a Pseudomonas aeruginosa O-polysaccharide toxin A conjugate vaccine in humans. J. Clin. Invest., 80, 51–6

    Article  PubMed  CAS  Google Scholar 

  48. Cryz, S. J. Jr., Sadoff, J. C. and Fürer, E. (1988). Immunization with a Pseudomonas aeruginosa immunotype 5 O polysaccharide-toxin A conjugate vaccine: effect of a booster dose on antibody levels in humans. Infect. Immun., 56, 1829–30

    PubMed  CAS  Google Scholar 

  49. Pier, G. B., Matthews, W. J., Jr and Eardley, D. D. (1983). Immunochemical characterization of the mucoid exopolysaccharide of Pseudomnas aeruginosa. J. Infect. Dis., 147, 494–503

    Article  PubMed  CAS  Google Scholar 

  50. Schwarzmann, D. and Boring, J. R. Jr (1971) Antiphagocytic effect of slime from a mucoid strain of Pseudomonas aeruginosa. Infect. Immun., 3, 762–7

    PubMed  CAS  Google Scholar 

  51. Ames, P., DesJardins, D. and Pier, G. B. (1985). Opsonophagocytic killing activity of rabbit antibody to Pseudomonas aeruginosa mucoid exopolysaccharide. Infect. Immun., 49, 281–5

    PubMed  CAS  Google Scholar 

  52. Pier, G. B., Saunders, J. M., Ames, P., Edwards, M. S., Auerbach, H., Goldfard, J., Speert, D. P. and Hurwitch, S. (1987) Opsonophagocytic killing antibody to Pseudomonas aeruginosa mucoid exopolysaccharide in older noncolonized patients with cystic fibrosis. N. Engl. J. Med., 317, 793–8

    Article  PubMed  CAS  Google Scholar 

  53. Nosocomial Infection Surveillance (1984). CDC morbidity and mortality weekly report 35, 17ss–29ss

    Google Scholar 

  54. Finland, M. (1930). The serum treatment of lobar pneumonia. N. Engl. J. Med., 202, 1244–7

    Article  Google Scholar 

  55. Pennington, J. E. and Pier, G. B. (1987). Pseudomonas aeruginosa immune globulin in experimental pneumonia. Infection, 15, S47–9

    Article  PubMed  Google Scholar 

  56. Counts, G. W., Schwartz, R. W., Ulness, B. K., Hamilton, D. J., Rosok, M. J., Dunningham, M. D., Tarn, M. R. and Darveau, R. P. (1988). Evaluation of an immunofluorescent-antibody test for rapid identification of Pseudomonas aeruginosa in blood cultures. J. Clin. Microbiol., 26, 1161–5

    PubMed  CAS  Google Scholar 

  57. Ziegler, E. J., McCutchan, J. A., Fierer, J., Glauser, M. P., Sadoff, J. C., Douglas, H. and Braude, A. I. (1982). Treatment of gram-negative bacteremia and shock with human antiserum to a mutant Escherichia coli. N. Engl. J. Med., 307, 1225–30

    Article  PubMed  CAS  Google Scholar 

  58. Greisman, S. E., DuBuy, J. B. and Woodward, C. L. (1979). Experimental gram-negative bacterial sepsis: prevention mortality not preventable by antibiotics alone. Infect. Immun., 25, 538–57

    PubMed  CAS  Google Scholar 

  59. Pennington, J. E. and Menkes, E. (1981). Type-specific versus cross-protective vaccination for gram-negative pneumonia. J. Infect. Dis., 144, 599–603

    Article  PubMed  CAS  Google Scholar 

  60. Sadoff, J. C., Futrovsky, S. L., Sidberry, H. F, Iglewski, B. H. and Seid, R. C. (1982). Detoxified lipopolysaccharide-protein conjugates. Sem. Infect. Dis., 4, 346–54

    CAS  Google Scholar 

  61. Cryz, S. J., Furer, E. and Germanier, R. (1984). Protection against fatal Pseudomonas aeruginosa burn wound sepsis by immunization with lipopolysaccharide and high-molecular-weight polysaccharide. Infect. Immun., 43, 795–9

    PubMed  CAS  Google Scholar 

  62. Pennington, J. E., Pier, G. B., Sadoff, J. C. and Small, G. J. (1986). Active and passive immunization strategies for Pseudomonas aeruginosa pneumonia. Rev. Infect. Dis., 8 (Suppl.), 426–33

    Article  Google Scholar 

  63. Holder, I. A. and Neely, A. N. (1987). Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: passive intravenous immunotherapy using Pseudomonas globulin. Serodiagnosis Immunol., 1, 153–62

    Article  Google Scholar 

  64. Collins, M. S. and Roby, R. E. (1984). Protective activity of an intravenous immune globulin (human) enriched in antibody against lipopolysaccharide antigens of Pseudomonas aeruginosa. Am. J. Med., 76, 168–74

    Article  PubMed  CAS  Google Scholar 

  65. Pennington, J. E., Pier, G. B. and Small, G. J. (1986). Efficacy of intravenous immune globulin for treatment of experimental Pseudomonas aeruginosa pneumonia. J. Crit. Care, 1, 4–10

    Article  Google Scholar 

  66. Class, I., Junginger, W. and Klöss, Th. (1987). Einsatz von pseudomonas-immunglobulin bei beatmeten patienten einer interdisziplinären chirurgischen intensivstation. Infection, 15 (Suppl. 2), S67–70

    Article  PubMed  Google Scholar 

  67. Sadoff, J. C., Wright, D. C., Futrovsky, S., Sidberry, H., Collins, H. and Kaufmann, B. (1985). Characterization of mouse monoclonal antibodies directed against Pseudomonas aeruginosa lipopolysaccharides. Antibiot. Chemother., 36, 134–146

    PubMed  CAS  Google Scholar 

  68. Sawada, S., Kawamura, T., Masuho, Y. and Tomibe, K. (1985). Characterization of a human monoclonal antibody to lipopolysaccharides of Pseudomonas aeruginosa serotype 5: a possible candidate as an immunotherapeutic agent for infections with P. aeruginosa. J. Infect. Dis., 5, 965–70

    Article  Google Scholar 

  69. Sawada, S., Kawamura, T. and Masuho, Y. (1987). Immunoprotective human monoclonal antibodies against five major serotypes of Pseudomonas aeruginosa. J. Gen. Microbiol., 133, 3581–90

    PubMed  CAS  Google Scholar 

  70. Zweerink, H. J., Gammon, M. C., Hutchison, C. F., Jackson, J. J., Lombardo, D., Miner, K. M., Puckett, J. M., Sewell, T. J. and Sigal, N. H. (1988). Human monoclonal antibodies that protect mice against challenge with Pseudomonas aeruginosa. Infect. Immun., 56, 1873–9

    PubMed  CAS  Google Scholar 

  71. Hector, R. F., Trawinski, J., Besemer, D., Collins, M. S. and Pennington, J. E. (1987). In vitro and in vivo evaluation of a human immunoglobulin M monoclonal antibody to Pseudomonas aeruginosa immunotype 3. Presented at the 27th Interscience Conference on Antimicrobial Agents and Chemotherapy, New York, No. 547

    Google Scholar 

Download references

Authors
  1. J. E. Pennington
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Medical Microbiology, London School of Hygiene and Tropical Medicine, University of London, London, UK

    A. J. Zuckerman

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Kluwer Academic Publishers

About this chapter

Cite this chapter

Pennington, J.E. (1989). Pseudomonas aeruginosa immunization. In: Zuckerman, A.J. (eds) Recent Developments in Prophylactic Immunization. Immunology and Medicine, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1067-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-1067-6_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6969-4

  • Online ISBN: 978-94-009-1067-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation