Abstract
The orientational order and reorientational dynamics of lipid bilayer systems have been studied by ESR techniques. The rigid nitroxide spin label cholestane has been used as a probe molecule. We show that the observed ESR spectra must be interpreted within the formalism of the stochastic Liouville equation, as the rates of the reorientational motions are slow on the ESR timescale. Furthermore, we find that the picture of the orientational order and the rotational dynamics obtained with ESR techniques agrees well with that obtained from fluorescence depolarization experiments. This indicates that the probe molecules do not perturb the local structure of the lipid matrix to a large extent, so that their behaviour reflects the intrinsic order and dynamics of the system. We show that the orientational order decreases and the rates of the rotational motions increase with hydration and temperature. In marked contrast, increasing unsaturation of the lipid chains induces a reduction in the orientational order only. Furthermore we find that the curvature of the bilayer in vesicle systems reduces the orientational order, but has little effect on the reorientational dynamics. These observations are at odds with the correlation between the orientational order and the reorientational dynamics postulated by current membrane models.
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References
Silver, B.L. (1985) in The Physical Chemistry of Membranes, Solomon Press, New York.
Redfield, A.G. (1965) in Advances in Magnetic Resonance vol. 1 (Waugh, J.S., ed.), pp. 1–32, Academic Press, New York.
Freed, J.H., Bruno, G.V. and Polnaszek, C.F. (1971), J. Phys. Chem. 75, 3385–3399.
Polnaszek, C.F., Bruno, G.V. and Freed, J.H. (1973), J. Chem. Phys. 58, 3185–3199.
Freed, J.H. (1976) in Spin Labeling. Theory and Applications, (Berliner, L.J. ed.) Academic Press, New York, pp. 53–132.
Lange, A., Marsh, D., Wassmer, K.H., Meier, P. and Kothe, G. (1985) Biochemistry 24, 4383–4392.
Mayer, C., Müller, K., Weisz, K. and Kothe, G. (1988) Liq. Crystals 3, 797–806.
Dammers, A.J. (1985) Ph.D. Thesis, Rijksuniversiteit Utrecht, Utrecht.
Freed, J.H. and Fraenkel, G.K. (1963), J. Chem. Phys. 39, 326–248
Vega, A.J. and Fiat, D. (1974) J. Chem. Phys. 60, 579–583.
Abragam, A. (1961) in The Principles of Nuclear Magnetism, Oxford Univ. Press, London.
Schneider, D.J. and Freed, J.H. (1989) in Advances in Chemical Physics, 73, 387–453.
Moro, G. and Freed, J.H. (1981) J. Chem. Phys. 74, 3757–3773.
Dammers, A.J., Levine, Y.K. and Tjon, J.A. (1988) J. Chem. Phys., 89, 4505–4513.
Gaffney, B.J. and McConnel, H.M. (1974), J. Magn. Reson. 16, 1–28.
Sachse, J.-H., King, M.D. and Marsh, D. (1987), J. Magn. Reson. 71, 385–404.
Seelig, A. and Seelig, J. (1977) Biochemistry 16, 45–50.
van Ginkel, G., van Langen, H. and Levine, Y.K., Biochimie (in press).
Ehrström, E. and Ehrenberg, A. (1983) Biochim. Biophys. Acta 735, 271–282.
Chapman, D., Byrne, P. and Shipley, G.G. (1966) Proc. R. Soc. London, A 290, 115–142.
Chapman, D. and Benga, G. (1984) in Biological Membranes V (Chapman, D. ed.) Academic Press, London, pp. 1–56.
Deinum, D., van Langen, H., van Ginkel, G. and Levine, Y.K. (1988) Biochemistry 27, 852–860.
Prendergast, F.G., Haugland, R.P. and Callahan, P.J. (1981) Biochemistry 20, 7333–7338.
van Langen et al. (1989) Eur. Biophys. J. (in press).
Rao, K.V.S., Polnaszek, C.F. and Freed, J.H. (1977) J. Phys. Chem. 81, 449–456.
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Korstanje, L.J., Van Faassen, E.E., Levine, Y.K. (1989). Electron Spin Resonance Studies of Reorientational Dynamics in Lipid Systems. In: Dorfmüller, T. (eds) Reactive and Flexible Molecules in Liquids. NATO ASI Series, vol 291. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1043-0_16
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DOI: https://doi.org/10.1007/978-94-009-1043-0_16
Publisher Name: Springer, Dordrecht
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