Abstract
A new method for gas chromatographic analysis of PQQ is presented. A sample was made weakly-alkaline and passed through a Sep-Pak C18 cartridge. The eluate was then made acid with HCl and passed through the second Sep-Pak C18 cartridge. Finally, PQQ was eluted with pyridine solution; it was evaporated to dryness. The residue was derivatized with a phenyltrimethylammonium reagent and subjected to gas chromatography (GC). The chemical structure of the reaction product was estimated to be 3-[1-methyl-3, 5-di(methoxycarbonyl)-pyrrole-2-yl]-2, 4, 6-tri(methoxycarbonyl)pyridine by mass spectral analysis. GC was made with SPB-1 fused silica wide-bore and narrow-bore capillary columns, and with flame ionization detection. The detection limit of PQQ was 5–10 ng in an injected volume with the narrow-bore capillary column in the splitless mode. The endogenous PQQ in human urine, plasma, brain and liver, and rat brain and liver, was below the detection limit (less than 100 ng/ml or g).
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© 1989 Kluwer Academic Publishers
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Suzuki, O., Kumazawa, T., Seno, H., Matsumoto, T., Urakami, T. (1989). Gas Chromatography of PQQ. In: Jongejan, J.A., Duine, J.A. (eds) PQQ and Quinoproteins. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0957-1_19
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DOI: https://doi.org/10.1007/978-94-009-0957-1_19
Publisher Name: Springer, Dordrecht
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