Abstract
Whey was hydrolysed by Kluyveromyces bulgaricus cells immobilized in either alginate or chitosan beads. The hydrolysate was used to grow Gluconobacter oxydans. The identification of the acids produced was carried out by Gas Capillary Chromatography. Glucose was oxidised to gluconic acid in less than 24 hours. Galactose was oxidised to galactonic acid at a lower rate.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ameyama M, Shinagawa E, Matsushita K and Adachi O, 1981. D-glucose dehydrogenase of Gluconobacter oxydans: solubilization, purification and characterization. Agric. Biol. Chem. 45: 851–861.
Anieyama M, Nonobe M, Shinagawa E, Matsushita K, Takimoto K and Adachi O, 1986. Purification and characterization of the quinoprotein D-glucose dehydrogenase apoenzyme from Escherichia coli. Agric. Biol. Chem. 50: 49–57.
Decleire M, Van Huynh N, Motte JC and De Cat W, 1985. Hydrolysis of whey by whole cells of Kluyveromyces butgaricus immobilized in calcium alginate gels and in hen egg white. Appl. Microbiol. Biotechnol. 22: 438–441.
Dokter P, Pronk JT, Van Schie BJ, Van Dijken JP and Duine JA, 1987. The in vivo and in vitro substrate specificity of quinoprotein glucose dehydrogenase of Acinetobacter calcoaceticus. FEMS Microbiology Letters 43: 195–200.
Homines RWJ, Loenen WAM, Neijssel OM and Postma PW, 1986. Galactose metabolism in gal mutants of Salmonella typhimurium and Escherichia coli. FEMS Microbiology Letters 36: 187–190.
Van Huynh N and Decleire M, 1982. Les cellules entieres de Kluyveromyces bulgaricus comme source de ß-galactosidase. Revue des Fermentations et des Industries Alimentaires. 37: 153–157.
Van Huynh N, Decleire M, Voets M, Motte JC and Monseur X, 1986. Production of gluconic acid from whey hydrolysate. Process Biochemistry 21: 31–32.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this chapter
Cite this chapter
Van Huynh, N., Decleire, M., Motte, J.C., Monseur, X. (1989). Production of Gluconic and Galactonic Acids from Whey. In: Jongejan, J.A., Duine, J.A. (eds) PQQ and Quinoproteins. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0957-1_14
Download citation
DOI: https://doi.org/10.1007/978-94-009-0957-1_14
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6920-5
Online ISBN: 978-94-009-0957-1
eBook Packages: Springer Book Archive