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Plant Molecular Biology Manual pp 29–47Cite as

Binary vectors

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Abstract

Agrobacterium tumefaciens is capable of transferring a defined piece of DNA (T-DNA) containing tumorigenic loci from its tumor-inducing (Ti) plasmid into the genome of a large number of gymnosperms and angiosperms [1–3]. This process requires the cis acting T-DNA border sequences [4–6] and the trans acting virulence (vir) functions encoded by the Ti plasmid and the Agrobacterium chromosome [7–10]. The transfer process is fully active when the vir functions and the T-DNA are located on separate compatible replicons in Agrobacterium [11]. These features made the development of binary vectors possible [12–15]. In such systems, the Agrobacterium host strain contains a wild-type Ti plasmid or disarmed (tumor genes deleted) Ti plasmid that carries the vir functions and serves a a helper. The T-DNA borders are located on a compatible replicón that will function in both E. coli and Agrobacterium. DNA that is inserted between the T-DNA borders will be efficiently transferred to and stably maintained within the plant genome.

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Author information

Authors and Affiliations

  1. Institute of Biological Chemistry and Plant Physiology Program, Washington State University, Pullman, WA, 99164-6340, USA

    Gynheung An, Paul R. Ebert, Amitava Mitra & Sam B. Ha

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  1. Gynheung An
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  2. Paul R. Ebert
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  3. Amitava Mitra
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  4. Sam B. Ha
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Editor information

Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Stanton B. Gelvin

  2. Leiden State Universiy, Leiden, The Netherlands

    Robbert A. Schilperoort

  3. Ohio State University, Columbus, Ohio, USA

    Desh Pal S. Verma

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© 1989 Kluwer Academic Publishers, Dordrecht

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An, G., Ebert, P.R., Mitra, A., Ha, S.B. (1989). Binary vectors. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_3

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  • DOI: https://doi.org/10.1007/978-94-009-0951-9_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6918-2

  • Online ISBN: 978-94-009-0951-9

  • eBook Packages: Springer Book Archive

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