Abstract
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA. The first enzymes described (type I) require various cofactors and lack sequence specificity for their sites of cleavage. However, a second class (type II) were discovered that require only Mg2+ asa cofactor and have the distinct advantage that they recognize and cleave very specific sequences. Type II restriction enzymes are therefore capable of reducing complex DNA, such as plant nuclear DNA, into a population of fragments with discrete sizes. At least 475 restriction endonucleases have been described to date [24].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aaij C, Borst P (1972) The gel electrophoresis of DNA. Biochim Biophys Acta 269: 192–200.
Arber W (1979) Promotion and limitation of genetic exchange. Science 205: 361–365.
Avise JC, Lansman RA (1983) Polymorphism of mitochondrial DNA in populations of higher animals. In: Nei M, Koehn RK (eds) Evolution of Genes and Proteins, pp 147–164. Sunderland, MA: Sinauer Assoc, Inc.
Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112: 887–898.
Bhattacharya SS, Wright AF, Clayton JF, Price WH, Phillips CI, McKeown CME, Jay M, Bird AC, Pearson J (1984) Close genetic linkage between X-linked Retinitis pigmentosa and a restriction fragment length polymorphism identified by recombinant DNA probe L1.28. Nature 309: 253–255.
Boyer HW (1971) DNA restriction and modification mechanisms in bacteria. Ann Rev Microbiol 25: 153–176.
Botstein D, White RL, Skolnick M, Davis R (1980) Construction of a genetic linkage map in man using restriction fragment polymorphisms. Am J Hum Genet 32: 314–331.
Burr B, Evola SV, Burr FA, Beckmann JS (1983) The application of restriction fragment length polymorphisms to plant breeding. In: Setlow JK, Hollaender A (eds) Genetic Engineering Principles and Methods, Vol 5, pp 45–59. New York: Plenum.
Chyi Y-S, Jorgensen RA, Goldstein D, Tanksley SD, Loaiza-Figueroa F (1986) Locations and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genome. Mol Gen Genet 204: 64–69.
Engels WR (1981) Estimating genetic divergence and genetic variability with restriction endo-nucleases. Proc Natl Acad Sci USA 78: 6329–6333.
Evola SV, Burr FA, Burr B (1986) The suitability of restriction fragment length polymorphisms as genetic markers in maize. Theor Appl Genet 71: 765–771.
Grodziker T, Williams J, Sharp P, Sambrook J (1974) Physical mapping of temperature-sensitive mutations of adenovirus. Cold Spring Harbor Symp Quant Biol 39: 439–446.
Gusella JF, Tanzi RE, Anderson MA, Hobbs W, Gibbons K, Raschtchian R, Gilliam TC, Wallace MR, Wexler NS, Conneally PM (1984) DNA markers for nervous system diseases. Science 225: 1320–1326.
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5: 109–118.
Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72: 761–769.
Hudson RR (1982) Estimating genetic variability with restriction endonucleases. Genetics 100: 711–719.
Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314: 67–73.
Leary JJ, Brigati J, Ward DC (1983) Rapid and sensitive method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci USA 80: 4045–4049.
Lederberg S, Meselson M (1964) Degredation of non-replicating bacteriophage DNA in non-accepting cells. J Mol Biol 8: 623–628.
Nei M, Tajima F (1985) Evolutionary change of restriction cleavage sites and phylogenetic inference in man and apes. Mol Biol Evol 2: 189–205.
Palmer JD, Jorgensen RA, Thompson WF (1985) Chloroplast DNA variation and evolution in Pisum: Patterns of change and phylogenetic analysis. Genetics 109: 195–213.
Polans NO, Weeden NF, Thompson WF (1985) Inheritance, organization and mapping of the rbcs and cab multigene families in pea. Proc Natl Acad Sci USA 32: 5083–5087.
Rigby PWJ, Dieckman M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 815–817.
Roberts RJ (1984) Restriction and modification enzymes and their recognition sequences. Nucl Acids Res 12: 167–191.
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517.
Southern EM (1979) Gel electrophoresis of restriction fragments. In: Wu R (ed) Methods of Enzymology 68: 152–176.
Tanksley SD (1983) Molecular markers in plant breeding. Plant Mol Biol Rep 1: 3–8.
Tanksley SD, Mutschier MA, Rick CM (1987) Linkage map of the tomato. In: O’Brien S (ed) Genetic Maps.
Templeton AR (1983) Phylogenetic inference from restriction endonuclease cleavage site maps with particular reference to the evolution of humans and apes. Evolution 37: 221–244.
Vallejos CE, Tanksley SD, Bernatzky R (1986) Localization in the tomato genome of DNA restriction fragments containing sequences homologous to the rRNA (45S), the major chlorophyll a/b binding polypeptide and the ribulose bisphosphate carboxylase genes. Genetics 112: 93–105.
Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzylmethalpaper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76: 3683–3687.
Wallroth M, Gerats AGM, Rogers SG, Fraley RT, Horsch RB (1986) Chromosomal localization of foreign genes in Petunia hybrida. Mol Gen Genet 202: 6–15.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers, Dordrecht
About this chapter
Cite this chapter
Bernatzky, R. (1989). Restriction fragment length polymorphism. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_24
Download citation
DOI: https://doi.org/10.1007/978-94-009-0951-9_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6918-2
Online ISBN: 978-94-009-0951-9
eBook Packages: Springer Book Archive