Abstract
The technique of genetic transformation of plant cells and regeneration of transgenic plants is possible in many species. The general objectives are to apply this technology in two major areas: the molecular definition of the genetic elements that regulate the expression of genes, and the rapid and directed genetic improvement of existing agricultural, horticultural, and ornamental varieties.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Jones J, Dunsmuir P, Bedbrook J (1985) High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J 4: 2411–2418.
Dunsmuir P, Bedbrook J, Bond D, Dean C, Gidoni D, Jones J (1987) The expression of introduced genes in regenerated plants. In: Setlow J, Hollaender A (Eds) Genetic Engineering, Vol. 9. New York: Plenum, in press.
Dunsmuir P, Smith S, Bedbrook J (1983) A number of different marker genes for the small subunit of RuBPCase are transmitted in petunia. Nucl Acids Res 11: 4177–4183.
Dunsmuir P, Smith S, Bedbrook J (1983) The major chlorophyll a/b binding protein of petunia is composed of several polypeptides encoded by a number of distinct nuclear genes. J Mol Appl Genet 2: 285–300.
Dean C, Favreaux M, Jones J, Dunsmuir P, Bedbrook J (1987) Expression of petunia rbcS gene fusions in transformed tobacco plants. In: Key J, Mcintosh L (eds) Proceedings UCLA Symposium, in press.
Ellis J, Llewellyn D, Dennis E, Peacock (1987) Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco. EMBO J 6: 11–16.
Chen Z, Schuler M, Beachy R (1986) Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci USA 83: 8560–8564.
McKnight (1982) Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus. Cell 31: 355–365.
Van den Elzen P, Townsend J, Lee K, Bedbrook J (1985) A chimaeric hygromycin resistance gene as a selectable marker in plant cells. Plant Mol Biol 5: 299–302.
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517.
Maniatis T, Fritsch E, Sambrook J (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers, Dordrecht
About this chapter
Cite this chapter
Dunsmuir, P., Bond, D., Lee, K., Gidoni, D., Townsend, J. (1989). Stability of introduced genes and stability in expression. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_23
Download citation
DOI: https://doi.org/10.1007/978-94-009-0951-9_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6918-2
Online ISBN: 978-94-009-0951-9
eBook Packages: Springer Book Archive