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Transformation of plant pathogenic fungi

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Plant Molecular Biology Manual

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Abstract

The ability to introduce exogenous DNA into living cells and demonstrate its expression is a requirement for many research programs whose goals include determining the function of specific genes. Plant pathogenic fungi are highly appropriate subjects for transformation experiments, because a number of fungal genes have been identified whose products are believed, but not proven, to play significant roles in interactions with host plants. For example, fungal enzymes which detoxify antimicrobial compounds produced by plants in response to infection are believed to be important to the pathogenicity of some fungi [46]; fungal enzymes which degrade cell wall components appear to play a role in pathogenicity [22]; and fungal Phytotoxins are hypothesized to serve as pathogenicity or virulence factors [49]. Isolation of the genes encoding these enzymes [40, 50], followed by their in vitro mutagenesis and reintroduction into fungal cells will permit conclusive analysis of their roles in disease. Transformation of fungi in general and plant pathogenic fungi in particular has advanced rapidly in recent years. This chapter presents a brief survey of progress in the field, a discussion of the various methods available for transformation of fungi, and a detailed protocol for transformation of fungal protoplasts.

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Author information

Authors and Affiliations

  1. Biotechnology Center and Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA

    Robert C. Garber

  2. Department of Plant Pathology, Cornell University, Ithaca, NY, 14853, USA

    B. Gillian Turgeon & Olen C. Yoder

Authors
  1. Robert C. Garber
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  2. B. Gillian Turgeon
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  3. Olen C. Yoder
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Editor information

Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Stanton B. Gelvin

  2. Leiden State Universiy, Leiden, The Netherlands

    Robbert A. Schilperoort

  3. Ohio State University, Columbus, Ohio, USA

    Desh Pal S. Verma

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© 1989 Kluwer Academic Publishers, Dordrecht

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Garber, R.C., Turgeon, B.G., Yoder, O.C. (1989). Transformation of plant pathogenic fungi. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_10

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  • DOI: https://doi.org/10.1007/978-94-009-0951-9_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6918-2

  • Online ISBN: 978-94-009-0951-9

  • eBook Packages: Springer Book Archive

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