Abstract
Nitrogen-fixing (acetylene-reducing) activities associated with rice plants obtained from field experiments using different N sources were measured by a modified short-term acetylene reduction assay. Besides using urea as source of inorganic N fertilizer, the following different organic fertilizers were incorporated in the treatments: grain legume green manure (Vigna radiata and Dolichos lablab), non-grain legume green manure (Sesbania and Crotolaria), non-legume green manure (Azolla), and rice straw. Nitrogen fixation per plant (or per unit area) was either enhanced or not affected by the application of inorganic N and organic fertilizers. However, when expressed on a per unit plant dry weight basis, the N2-fixing activities were generally lower, except in cases where some legume green manures such as V. radiata and Crotolaria were incorporated. Possible mechanisms of how rice plant associative N2 fixation is affected by different N fertilizers are discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Balandreau J, Rinaudo G, Fares-Hamad, J and Dommergues Y 1975 N2 fixation in paddy soils. In Nitrogen Fixation by Free-living Microorganisms. IBP Series No. 6, 57–70.
Dreyfus B L, Elmerich C and Dommergues Y R 1983 Freeliving Rhizobium strain able to grow on N2 as the sole nitrogen source. Appl. Environ. Microbiol. 45, 711–713.
Gilmour J T, Gilmour C M, and Johnston T H 1978 Nitrogenase activity of rice plant root system. Soil Biol. Biochem. 10, 261–264.
Koyama T and App A A 1979 Nitrogen balance in flooded rice soils. In Nitrogen and Rice. pp. 95–104. International Rice Research Institute, P.O. Box 933, Manila, Philippines.
Ladha J K, Tirol-Padre A, Daroy M L, Punzalan G and Ventura W 1986 Plant-associated N2 fixation (C2H2-reduction) by five rice varieties, and relationship with plant growth characters as affected by straw incorporation. Soil Sci. Plant Nutr. 32, 91–106.
Ladha J K, Tirol-Padre A, Daroy M L, Punzalan G and Ventura W 1986 Plant-associated N2 fixation (C2H2-reduction) by five rice varieties, and relationship with plant growth characters as affected by straw incorporation. Soil Sci. Plant Nutr. 32, 91–106.
Ladha J K, Tirol-Padre A, Punzalan G C and Watanabe I 1987 Nitrogen-fixing (C2H2-reducing) activity and plant growth characters of 16 wetland rice varieties. Soil Sci. Plant Nutr. 33, 187–200.
Matsuguchi T 1977 Factors affecting heterotrophic nitrogen fixation in submerged rice soils. In Nitrogen and Rice. pp. 207–222. International Rice Research Institute. P.O. Box 933, Philippines.
Nagarajah S 1987 Transformation of green manure nitrogen in lowland rice soils. Proc. Symposium on Sustainable Agriculture—The Role of Green Manure Crops in Rice Farming Systems. CASAFA/IRRI, 25–29 May 1987. (In press).
Neue H 1985 Organic matter dynamics in wetland soils. In Wetland Soils: Characterization, Classification and Utilization. pp. 109–122. International Rice Research Institute, P.O. Box 933, Manila, Philippines.
Ponnamperuma F N 1984 Straw as source of nutrients for wetland rice. In Organic Matter and Rice. pp. 117–136. International Rice Research Institute, P.O. Box 933, Manila, Philippines.
Rao J L N, Prasad J S and Rajaramamohan Rao V 1982 Rice rhizosphere nitrogen fixation (C2H2 reduction) as influenced by nitrogen source. Curr. Sci. 50, 900–901.
Rao J L N, Reddy B B, Rajaramamohan Rao V 1983 Rhizosphere soil nitrogenase (C2H2 reduction) as influenced by the nitrogen management in intermediate deep water rice. J. Agric. Camb. 101, 547–551.
Roger P A and Kulasooriya S A 1980 Blue-green Algae and Rice. pp. 112. International Rice Research Institute, P.O. Box 933, Manila, Philippines.
Tirol-Padre A, Ladha J K, Punzalan G C and Watanabe I (1987) A plant sampling procedure for acetylene reduction assay to detect rice varietal differences in ability to stimulate N2 fixation. Soil Biol. Biochem. (In press)
Trolldenier G 1977 Influence of some environmental factors on nitrogen fixation in the rhizosphere of rice. Plant and Soil 47, 203–217.
Watanabe I, De Guzman M R and Cabrera D A 1981 The effect of nitrogen fertilizer on N2 fixation in the paddy field measured by in situ acetylene reduction assay. Plant and Soil 59, 135–139.
Watanabe I 1986 Nitrogen fixation by non-legumes in tropical agriculture with special reference to wetland rice. Plant and Soil 90, 343–357.
Watanabe I and Inubushi K 1986 Dynamics of available nitrogen in paddy soils. Soil Sci. Plant Nutr. 32, 37–50.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this chapter
Cite this chapter
Ladha, J.K., Tirol-Padre, A., Punzalan, G.C., Garcia, M., Watanabe, I. (1989). Effect of inorganic N and organic fertilizers on nitrogen-fixing (acetylene-reducing) activity associated with wetland rice plants. In: Skinner, F.A., Boddey, R.M., Fendrik, I. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 35. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0889-5_31
Download citation
DOI: https://doi.org/10.1007/978-94-009-0889-5_31
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6888-8
Online ISBN: 978-94-009-0889-5
eBook Packages: Springer Book Archive