Abstract
A nitrogen fixing organism containing a plasmid has been isolated from the rhizosphere fraction of Leptochloa fusca (L) Kunth (kallar grass) growing on saline soils in the Punjab area. This bacterium can grow aerobically in a medium containing 1 M NaCl and can fix nitrogen efficiently under microaerobic conditions on semi-solid medium with glucose or sucrose as a carbon source. Maximum N2-fixation in batch cultures occurred with 100 mM NaCl at pH 8.0 and 35°C. DNA hybridization and analysis of the protein pattern were carried out to establish its taxonomic position. On the basis of protein electrophoretic pattern, physiological characteristics, DNA relatedness, and better growth in the presence of high NaCl concentration, we regard this strain as a new species of Klebsiella.
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References
Bagley ST, Seidler RJ and Brenner DJ 1981 Klebsiella planticola sp. nov.: A new species of Enterobacteriacae found primarily in non-clinical environments. Curren. Microbiol. 6, 105–109.
Bergersen FJ, Kennedy C and Hill S 1981 Influence of low oxygen concentration on depression of nitrogenase in Klebsiella pneumoniae. J. Gen. Microbiol. 128, 909–915.
Bilal R and Malik KA 1987 Isolation and identification of a nitrogen fixing zoogloea forming bacterium from Kallar grass histoplane. J. Appl. Bacteriol. 62, 289–294.
Dart PJ 1986 Nitrogen fixation associated with non-legumes in agriculture. Plant and Soil 90, 303–334.
Gowa J 1953 A micro biuret method for protein determination. Scan. J. Clin. Lab. Invest. 5, 218–222.
Hill S 1976 Influence of oxygen concentration on acetylene reduction and efficiency of nitrogen fixation in intact Klebsiella pneumoniae. J. Gen. Microbiol. 93, 335–345.
Hill S, Turner GL and Bergersen FJ 1984 Synthesis and activity of nitrogenase in Klebsiella pneumoniae exposed to low concentrations of oxygen. J. Gen. Microbiol. 130, 1061–1067.
Izard D, Ferragut F, Kersters K, De Ley J and Leclerc H 1981 Klebsiella terrigina: A new species from soil and water. Int. J. Syst. Bacteriol. 31, 116–127.
Kersters K and De Ley J 1975 Identification and grouping of bacteria by numerical analysis of their electrophoretic patterns. J. Gen. Microbiol. 87, 333–342.
Kersters K and De Ley J 1980 Classification and identification of bacteria by electrophoresis of their proteins. In Microbiological Classification and Identification. Eds. M. Goodfellow and RG Board, pp. 273–297. Soc. Appl. Bacteriol. Symp. Ser. 8. Academic Press, London.
Klucas R 1972 Nitrogen fixation grown in the presence of oxygen. Can. J. Microbiol. 18, 1845–1850.
Laemmli UK 1970 Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature (London) 27, 680–685.
Ladha JK, Barraquio WL and Watanabe I 1983 Isolation and identification of nitrogen-fixing Enterobacter cloacae and Klebsiella planticola with rice plants. Can. J. Microbiol. 29, 1301–1308.
Malik KA, Zafar Y and Hussain A 1980 Nitrogenase activity in the rhizosphere of Kallar grass. Biologia 26, 107–112.
Malik KA, Zafar Y and Hussain A 1982 Associative dinitrogen fixation in Diplachne fusca (Kallar grass). In Biological Nitrogen Fixation Technology for Tropical Agriculture. Eds. PH Graham and SC Harris, pp. 503–507. Cali, Colombia. CIAT.
Marmour J 1961 A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208–218.
McConaughy BC, Laird CD and McCarthy BJ 1969 Nucleic acid reassociation in formamide. Biochemistry 18, 3289–3295.
Reinhold B, Hurek T, Fendrik I, Pot B, Gills M, Kersters K, Thielemans S and De Ley J 1987 Azospirillum halopraeferens sp. nov.: A nitrogen fixing organism associated with roots of Kallar grass (Leptochloa fusca (L) Kunth.) Int. J. Sys. Bacteriol. 37, 43–51.
Sandhu GR and Malik KA 1975 Plant succession, key to utilization of salt affected soils. Nucleus 12, 35–38.
Shavikumar AG, Grundling GJ, Benson TA, Casuto D, Miller MF and Spear BB 1986 Vegetative expression of endotoxin genes of Bacillus thuringiensis sub sp. Kurstaki in Bacillus subtilus J. Bacteriol. 116, 194–204.
Steigerwalt AG, Fanning GR, Fife-Ausbury MA and Brenner DJ 1976 DNA relatedness among species of Enterobacter and Serratia, Can. J. Microbiol. 22, 121–127.
Yoch DC and Pengra RM 1966 Effect of amino acids on the nitrogenase system of Klebsiella pneumoniae J. Bacteriol. 92, 618–622.
Zafar Y 1985 Studies on the diazotrophic biocoeonosis in Kallar grass (Leptochloa fusca) (L) Kunth). Ph.D thesis. Quaid-i-Azam University, Islamabad, Pakistan.
Zafar Y, Ashraf M and Malik KA 1986 Nitrogen fixation associated with roots of Kallar grass (Leptochloa fusca (L) Kunth). Plant and Soil 90, 102–105.
Zafar Y, Malik KA and Niemann EG 1987 Studies on N2-fixing bacteria associated with the salt tolerant grass, Leptochloa fusca (L) Kunth. MIRCEN J. Appl. Bacteriol. Biotech. 3, 45–56.
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© 1989 Kluwer Academic Publishers
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Qureshi, J.A., Zafar, Y., Malik, K.A. (1989). Klebsiella sp. NIAB-I: A new diazotroph, associated with roots of kallar grass from saline sodic soils. In: Skinner, F.A., Boddey, R.M., Fendrik, I. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 35. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0889-5_15
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DOI: https://doi.org/10.1007/978-94-009-0889-5_15
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