Molecular genetics of rotifers: preliminary restriction mapping of the mitochondrial genome of Brachionus plicatilis

  • Charles E. King
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 52)


Methods are presented to extract and purify mitochondrial DNA from the rotifer Brachionus plicatilis. The mtDNA obtained is of sufficient purity for digestion with restriction endonucleases. EcoR I restriction patterns are presented for 4 geographically separated clones. A restriction map based on digestion with 5 different restriction enzymes is included for one of these clones. Finally, use of mtDNA analysis for studies on the population structure and biogeography of rotifers is discussed.

Key words

mitochondrial DNA genetic variation restriction mapping rotifers Brachionus plicatilis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Avise, J. C., J. Arnold, R. M. Ball, E. Bermingham, T. Lamb, J. E. Neigel, G A. Reeb & N. G Saunders, 1987. Intra-specific phylogeography: The mitochondrial bridge between population genetics and systematics. Ann. Rev. Ecol. Syst. 18: 489–522.Google Scholar
  2. Brown, W. M., 1985. The mitochondrial genome of animals. In: MacIntyre, R.J. (Ed.). Molecular Evolutionary Genetics. Plenum Press, New York: 95–130.Google Scholar
  3. Caccone, A., G. D. Amato & J. R. Powell, 1988. Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup. Genetics 118: 671–683.PubMedGoogle Scholar
  4. Dumont, H. J., 1983. Biogeography of rotifers. Hydrobiologia 104: 19–30.CrossRefGoogle Scholar
  5. Garesse, R., 1988. Drosophila melanogaster mitochondrial DNA: Gene organization and evolutionary considerations. Genetics 118: 649–663.PubMedGoogle Scholar
  6. Hillis, D. M., 1987. Molecular versus morphological approaches to systematics. Ann. Rev. Ecol. Syst. 18: 23–42.CrossRefGoogle Scholar
  7. Ishikaea, H., 1977. Comparative studies on the thermal stability of animal ribosomal RNA’s. IV. Thermal stability and molecular integrity of ribosomal RNA’s from several protostomes (rotifers, round-worms, liver-flukes, and brine shrimps). Comp. Biochem. Physiol. 56: 229–234.CrossRefGoogle Scholar
  8. King, C. E., 1977. Genetics of reproduction, variation, and adaptation in rotifers. Arch. Hydrobiol. Ergebn. Limnol. 8: 187–201.Google Scholar
  9. King, C. E., 1980. The genetic structure of zooplankton populations. In: Kerfoot, W. C. (Ed.). Evolution and Ecology of Zooplankton Communities. New England University Press: 315–329.Google Scholar
  10. King, C. E. & Y. Zhao, 1987. Coexistence of rotifer (Brachionus plicatilis) clones in Soda Lake, Nevada. Hydrobiologia 147: 57–64.CrossRefGoogle Scholar
  11. King, C. E., Y. Zhao, X. Liu & M. R. Li, 1988a. Polyacrylamide gel electrophoresis of isozyme variation in Artemia from Chinese salterns. J. Shandong College of Oceanography 18: 64–69.Google Scholar
  12. King, C. E., Y. Zhao, X. Liu & M. R. Li, 1988b. Genetic variation in parthenogenetic Artemia from the Shandong Peninsula, P.R.C. Oceanology and Limnology 6: 179–185.CrossRefGoogle Scholar
  13. Kumazaki, T., H. Hori, S. Osawa, N. Ishii & K. Suzuki, 1982. The nucleotide sequences of 5S rRNA’s from a rotifer, Brachionus plicatilis, and two nematodes, Rhabditis tokai and Caenorhabditis elegans. Nucleic Acids Res. 10: 7001–7004.PubMedCrossRefGoogle Scholar
  14. Lair, N., 1980. The rotifer fauna of the river Loire (France), at the level of the nuclear power plants. Hydrobiologia 73: 153–160.CrossRefGoogle Scholar
  15. MacNeil, D. & C. Strobeck, 1987. Evolutionary relationships among colonies of Columbian ground squirrels as shown by mitochondrial DNA. Evolution 41: 873–881.CrossRefGoogle Scholar
  16. May, L., 1983. Rotifer occurrence in relation to water temperature in Loch Leven, Scotland. Hydrobiologia 104: 311–315.CrossRefGoogle Scholar
  17. Moritz, C., T. E. Dowling & W. M. Brown, 1987. Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Ann. Rev. Ecol. Syst. 18: 269–292.CrossRefGoogle Scholar
  18. Pejler, B., 1980. Variation in the genus Keratella. Hydrobiologia 73: 207–213.CrossRefGoogle Scholar
  19. Ruttner-Kolisko, A., 1983. The significance of mating processes for the genetics and for the formation of resting eggs in monogonont rotifers. Hydrobiologia 104: 181–190.CrossRefGoogle Scholar
  20. Serra-Galindo, M., 1987. Variación morfométrica, isoenzimática y demográfica en poblaciones de Brachionus plicatilis: Diferenciación genética y plasticidad fenotipica. Ph.D. Thesis, Universitat de Valencia.Google Scholar
  21. Zhao, Y. & C. E. King. Ecological genetics of the rotifer Brachionus plicatilis in Soda Lake, Nevada, U.S.A. Hydrobiologia in press.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Charles E. King
    • 1
  1. 1.Dept. of ZoologyOregon State UniversityCorvallisUSA

Personalised recommendations