Abstract
Methods are presented to extract and purify mitochondrial DNA from the rotifer Brachionus plicatilis. The mtDNA obtained is of sufficient purity for digestion with restriction endonucleases. EcoR I restriction patterns are presented for 4 geographically separated clones. A restriction map based on digestion with 5 different restriction enzymes is included for one of these clones. Finally, use of mtDNA analysis for studies on the population structure and biogeography of rotifers is discussed.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Avise, J. C., J. Arnold, R. M. Ball, E. Bermingham, T. Lamb, J. E. Neigel, G A. Reeb & N. G Saunders, 1987. Intra-specific phylogeography: The mitochondrial bridge between population genetics and systematics. Ann. Rev. Ecol. Syst. 18: 489–522.
Brown, W. M., 1985. The mitochondrial genome of animals. In: MacIntyre, R.J. (Ed.). Molecular Evolutionary Genetics. Plenum Press, New York: 95–130.
Caccone, A., G. D. Amato & J. R. Powell, 1988. Rates and patterns of scnDNA and mtDNA divergence within the Drosophila melanogaster subgroup. Genetics 118: 671–683.
Dumont, H. J., 1983. Biogeography of rotifers. Hydrobiologia 104: 19–30.
Garesse, R., 1988. Drosophila melanogaster mitochondrial DNA: Gene organization and evolutionary considerations. Genetics 118: 649–663.
Hillis, D. M., 1987. Molecular versus morphological approaches to systematics. Ann. Rev. Ecol. Syst. 18: 23–42.
Ishikaea, H., 1977. Comparative studies on the thermal stability of animal ribosomal RNA’s. IV. Thermal stability and molecular integrity of ribosomal RNA’s from several protostomes (rotifers, round-worms, liver-flukes, and brine shrimps). Comp. Biochem. Physiol. 56: 229–234.
King, C. E., 1977. Genetics of reproduction, variation, and adaptation in rotifers. Arch. Hydrobiol. Ergebn. Limnol. 8: 187–201.
King, C. E., 1980. The genetic structure of zooplankton populations. In: Kerfoot, W. C. (Ed.). Evolution and Ecology of Zooplankton Communities. New England University Press: 315–329.
King, C. E. & Y. Zhao, 1987. Coexistence of rotifer (Brachionus plicatilis) clones in Soda Lake, Nevada. Hydrobiologia 147: 57–64.
King, C. E., Y. Zhao, X. Liu & M. R. Li, 1988a. Polyacrylamide gel electrophoresis of isozyme variation in Artemia from Chinese salterns. J. Shandong College of Oceanography 18: 64–69.
King, C. E., Y. Zhao, X. Liu & M. R. Li, 1988b. Genetic variation in parthenogenetic Artemia from the Shandong Peninsula, P.R.C. Oceanology and Limnology 6: 179–185.
Kumazaki, T., H. Hori, S. Osawa, N. Ishii & K. Suzuki, 1982. The nucleotide sequences of 5S rRNA’s from a rotifer, Brachionus plicatilis, and two nematodes, Rhabditis tokai and Caenorhabditis elegans. Nucleic Acids Res. 10: 7001–7004.
Lair, N., 1980. The rotifer fauna of the river Loire (France), at the level of the nuclear power plants. Hydrobiologia 73: 153–160.
MacNeil, D. & C. Strobeck, 1987. Evolutionary relationships among colonies of Columbian ground squirrels as shown by mitochondrial DNA. Evolution 41: 873–881.
May, L., 1983. Rotifer occurrence in relation to water temperature in Loch Leven, Scotland. Hydrobiologia 104: 311–315.
Moritz, C., T. E. Dowling & W. M. Brown, 1987. Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Ann. Rev. Ecol. Syst. 18: 269–292.
Pejler, B., 1980. Variation in the genus Keratella. Hydrobiologia 73: 207–213.
Ruttner-Kolisko, A., 1983. The significance of mating processes for the genetics and for the formation of resting eggs in monogonont rotifers. Hydrobiologia 104: 181–190.
Serra-Galindo, M., 1987. Variación morfométrica, isoenzimática y demográfica en poblaciones de Brachionus plicatilis: Diferenciación genética y plasticidad fenotipica. Ph.D. Thesis, Universitat de Valencia.
Zhao, Y. & C. E. King. Ecological genetics of the rotifer Brachionus plicatilis in Soda Lake, Nevada, U.S.A. Hydrobiologia in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this paper
Cite this paper
King, C.E. (1989). Molecular genetics of rotifers: preliminary restriction mapping of the mitochondrial genome of Brachionus plicatilis . In: Ricci, C., Snell, T.W., King, C.E. (eds) Rotifer Symposium V. Developments in Hydrobiology, vol 52. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0465-1_46
Download citation
DOI: https://doi.org/10.1007/978-94-009-0465-1_46
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6694-5
Online ISBN: 978-94-009-0465-1
eBook Packages: Springer Book Archive