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Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment

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Plant Tissue Culture Manual

Abstract

Genetic tranformation technologies are essential to programs of molecular biology and genetic engineering. Although significant efforts in conifer molecular biology have been initiated since the late 1980s, so far only a limited number of genes have been cloned [1]. Conifers are by far the most difficult plant group for this type of study because of their large genomes and lengthy life cycles. Furthermore, progress has been hindered by the present inefficiencies in gene transfer methods and tissue culture protocols for certain species such as pines. Nevertheless, there is a significant body of literature on gene transfer in several conifer species using Agrobacterium-mediated transformation and other protocols of direct DNA transfer.

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Authors and Affiliations

  1. Molecular Genetics and Tissue Culture Group; Petawawa National Forestry Institute, Box 2000, Chalk River, Ontario, K0J 1J0, Canada

    Armand Séguin, Denis Lachance & Pierre J. Charest

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  1. Armand Séguin
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  2. Denis Lachance
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  3. Pierre J. Charest
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  1. Department of Biological Sciences, University of Durham, UK

    K. Lindsey

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© 1996 Kluwer Academic Publishers

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Séguin, A., Lachance, D., Charest, P.J. (1996). Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0181-0_2

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  • DOI: https://doi.org/10.1007/978-94-009-0181-0_2

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