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Regulation of a class of auxin-induced genes in cell-suspension cultures from Nicotiana tabacum

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Plant Hormone Signal Perception and Transduction

Abstract

Protoplasts derived from transgenic tobacco suspension cells, harbouring a chimaeric gene construct consisting of an auxin-inducible promoter and the GUS coding region as a reporter gene, maintained their ability to respond to auxins. Antibodies directed against the presumed auxin-binding site of the major maize auxin-binding protein were recently obtained and shown to have auxin-agonist activity in mesophyll protoplasts from tobacco. This auxin-like induction of the hyperpolarisation of the plasma membrane potential by these D16 antibodies was confirmed in this study by time resolved analysis for tobacco cell-suspension protoplasts. In contrast, the same antibodies were able to inhibit auxin-induced expression of the chimaeric gene construct and to reduce the accumulation of [14C]2,4–dichlorophenoxyacetic acid (2,4–D) in transgenic tobacco protoplasts. A possible role for an auxin-influx carrier in transmitting the auxin signal will be discussed.

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References

  1. Ainley WM, Walker JC, Nagao RT and Key JL (1988) Sequence and characterization of two auxin-regulated genes from soybean. J Biol Chem 263: 10658–10666

    PubMed  CAS  Google Scholar 

  2. Barbier-Brygoo H, Ephritikhine G, Klämbt D, Ghislain M and Guern J (1989) Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86: 891–895

    Article  PubMed  CAS  Google Scholar 

  3. Boot CJM, Van Der Zaal EJ, Velterop J, Quint A, Mennes AM, Hooykaas PJJ and Libbenga KR (1993) Further characterization of expression of auxin-induced genes in tobacco (Nicotiana tabacum) cell-suspension cultures. Plant Physiol 102:513–520

    PubMed  CAS  Google Scholar 

  4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254

    Article  PubMed  CAS  Google Scholar 

  5. Goldsmith MHM (1977) The polar transport of auxin. Ann Rev Plant Phsyiol 28: 179–187

    Google Scholar 

  6. Hagen G, Kleinschmidt A and Guilfoyle TJ (1984) Auxin-regulated gene expression in intact soybean hypocotyl and excised hypocotyl sections. Planta 162: 147–153

    Article  CAS  Google Scholar 

  7. Hesse T, Feldwisch J, Balshüsemann D, Bauw G, Puype M, Vandekerckhove J, Löbler M, Klämbt D, Schell J and Palme K (1989) Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin. EMBO J 8: 2453–2461

    PubMed  CAS  Google Scholar 

  8. Ince C, Van Dissel JT and Diesselhof-den Dulk MMC (1985) A teflon culture dish for high magnification microscopy and measurements in single cells. Pflügers Arch 403: 240–244

    Article  PubMed  CAS  Google Scholar 

  9. Inohara N, Shimomura S, Fukui T and Futai M (1989) Auxin-binding protein located in the endoplasmic reticulum of maize shoots: Molecular cloning and complete primary structure. Proc Natl Acad Sci USA 86: 3564–3568

    Article  PubMed  CAS  Google Scholar 

  10. Jefferson RA (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387–405

    Article  CAS  Google Scholar 

  11. Koens KB (1993) The growth regulator 2,4–D as a signal molecule in tobacco cell cultures. PhD Thesis, Leiden University, The Netherlands

    Google Scholar 

  12. Kotyk A, Janacek K and Koryta J (1988) In: Biophysical chemistry of membrane functions. Eds J Wiley and sons

    Google Scholar 

  13. McClure BA, Hagen G, Brown CS, Gee MA and Guilfoyle TJ (1989) Transcription, organization and sequence of an auxin-regulated gene cluster in soybean. Plant Cell 1: 229–239

    Article  PubMed  CAS  Google Scholar 

  14. Napier RM, Venis MA, Bolton MA, Richardson LI and Butcher GW (1988) Preparation and characterisation of monoclonal and polyclonal antibodies to maize membrane auxin-binding protein. Planta 176: 519–526

    Article  CAS  Google Scholar 

  15. Raven JA (1975) Transport of indole acetic acid in plant cells in relation to pH and electrical potential gradients and its significance for polar IAA transport. New Phytol 74: 163–172

    Article  CAS  Google Scholar 

  16. Rubery PH (1978) Hydrogen ion dependence of carrier-mediated auxin uptake by suspension-cultured crown guard cells. Planta 142:203–206

    Article  CAS  Google Scholar 

  17. Rubery PH (1987) In: Davies PJ (ed) Plant hormones and their role in plant growth and development, pp 341–362. Martinus Nijhoff, Dordrecht

    Google Scholar 

  18. Rück A, Palme K, Venis MA, Napier RM and Felle HH (1993) Patch-clamp analysis establishes a role for an auxin binding protein in the auxin stimulation of plasma membrane current in Zea mays protoplasts. Plant J 4: 41–46

    Article  Google Scholar 

  19. Shimomura S, Sotobayashi T, Futai M and Fukui T (1986) Purification and properties of an auxin-binding protein from maize shoot membranes. J Biochem 99: 1513–1524

    PubMed  CAS  Google Scholar 

  20. Takahashi Y, Kuroda H, Tanaka T, Machida Y, Takebe I and Nagata T (1989) Isolation of an auxin-regulated gene cDNA expressed during the transition from G0 to S phase in tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86: 9279–9383

    Article  PubMed  CAS  Google Scholar 

  21. Thomson KL, Hertel R, Müller S and Tavares JE (1973) N–1–Naphthylphthalamic acid and 2,3,5–triiodobenzoic acid. In vitro binding to particulate cell fractions and action on auxin transport in corn coleoptiles. Planta 109: 337–352

    Article  CAS  Google Scholar 

  22. Tillman U, Viola G, Kayser B, Siemeister G, Hesse T, Palme K, Löbler M and Klämbt D (1989) cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): Isolation and characterization by immunological methods. EMBO J 8: 2463–2467

    Google Scholar 

  23. Van Der Zaal EJ, Memelink J, Mennes AM, Quint A and LibbengaKR (1987) Auxin-inducedmRNA species in tobacco cell cultures. Plant Mol Biol 10: 145–157

    Article  Google Scholar 

  24. Van DerZaal EJ, Droog FNJ, Boot CJM, Hensgens LAM, Hoge JHC, Schilperoort RA and Libbenga KR (1991) Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression. Plant Mol Biol 16: 983–998

    Article  Google Scholar 

  25. Van Duijn B, Ypey DL and Van der Molen LG (1988) Electrophysiological properties of Dictyostelium derived from membrane potential measurements with microelectrodes. J Membrane Biol 106: 123–134

    Article  Google Scholar 

  26. Van Slogteren GMS, Hoge JHC, Hooykaas PJJ and Schilperoort RA (1983) Clonal analysis of heterogeneous crown gall tumor tissues induced by wild-type and shooter mutant strains of Agrobacterium tumefaciens-expression of T-DNA genes. Plant Mol Biol 2: 321–333

    Article  Google Scholar 

  27. Venis MA, Napier RM, Barbier-Brygoo H, Maurel C, Perrot-Rechenmann C and Guern J (1992) Antibodies to a peptide from the maize auxin-binding protein have auxin agonist activity. Proc Natl Acad Sci USA 89: 7208–7212

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands

    C. J. M. Boot, B. van Duijn, A. M. Mennes & K. R. Libbenga

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  1. C. J. M. Boot
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  2. B. van Duijn
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  3. A. M. Mennes
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  4. K. R. Libbenga
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Editor information

Editors and Affiliations

  1. Institute of Biological Sciences, University of Wales, Aberystwyth, UK

    A. R. Smith , A. W. Berry , N. V. J. Harpham  & M. A. Hall , ,  & 

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

    I. E. Moshkov , G. V. Novikova  & O. N. Kulaeva ,  & 

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© 1996 Kluwer Academic Publishers

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Boot, C.J.M., van Duijn, B., Mennes, A.M., Libbenga, K.R. (1996). Regulation of a class of auxin-induced genes in cell-suspension cultures from Nicotiana tabacum . In: Smith, A.R., et al. Plant Hormone Signal Perception and Transduction. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0131-5_7

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  • DOI: https://doi.org/10.1007/978-94-009-0131-5_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6546-7

  • Online ISBN: 978-94-009-0131-5

  • eBook Packages: Springer Book Archive

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