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Plant Hormone Signal Perception and Transduction pp 15–22Cite as

Expression of an auxin-inducible promoter of tobacco in Arabidopsis thaliana

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Abstract

The expression of the auxin-inducible Nt103–1 gene of tobacco was studied in Arabidopsis thaliana. For this purpose we introduced a gene fusion between the promoter of the gene and the β-glucuronidase reporter gene (GUS) into Arabidopsis thaliana. The expression and location of GUS activity were studied histochemically in time and after incubation of seedlings on medium containing auxins or other compounds. The auxins 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), and 1-naphthylacetic acid (1-NAA) were able to induce GUS activity in the root tips of transgenic seedlings. The auxin transport inhibitor 2,3,5-triiodobenzoic acid was able to induce GUS activity not only in the root tip, but also in other parts of the root. Induction by the inactive auxin analog 3,5-dichlorophenoxyacetic acid was much weaker. Compounds like glutathione and the heavy metal CuSO4 were weak inducers. GUS activity observed after induction by glutathione was located in the transition zone. Salicylic acid and compounds increasing the concentration of hydrogen peroxide in the cell were also very well able to induce GUS activity in the roots. The possible involvement of hydrogen peroxide as a second messenger in the pathway leading to the induction of the Nt103–1 promoter is discussed.

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Authors and Affiliations

  1. Hooykaas Institute of Molecular Plant Sciences, Center for Phytotechnology RUL/TNO, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands

    Dianne A. M. van der Kop, Frans N. J. Droog, Bert J. van der Zaal & Paul J. J. Hooykaas

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  1. Dianne A. M. van der Kop
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  2. Frans N. J. Droog
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  3. Bert J. van der Zaal
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  4. Paul J. J. Hooykaas
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Editors and Affiliations

  1. Institute of Biological Sciences, University of Wales, Aberystwyth, UK

    A. R. Smith , A. W. Berry , N. V. J. Harpham  & M. A. Hall , ,  & 

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

    I. E. Moshkov , G. V. Novikova  & O. N. Kulaeva ,  & 

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© 1996 Kluwer Academic Publishers

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van der Kop, D.A.M., Droog, F.N.J., van der Zaal, B.J., Hooykaas, P.J.J. (1996). Expression of an auxin-inducible promoter of tobacco in Arabidopsis thaliana . In: Smith, A.R., et al. Plant Hormone Signal Perception and Transduction. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0131-5_3

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  • DOI: https://doi.org/10.1007/978-94-009-0131-5_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6546-7

  • Online ISBN: 978-94-009-0131-5

  • eBook Packages: Springer Book Archive

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