Abstract
Purified preparations of FC receptors from maize, obtained under non-denaturing conditions, showed in SDS-PAGE two doublets of proteins with an apparent molecular mass of 30 and 90 kDa. In this paper the isolation of the 30 kDa protein, its identification as a 14–3–3-like protein, as well as its immunological detection in partially-purified FC-receptor preparations from bean and spinach are described. The 14–3–3 proteins have biochemical properties consistent with potential signalling roles, and their presence in highly purified FC-receptor preparations suggests that they may be involved in FC- signal transduction. Photoaffinity labelling experiments demonstrating that the protein at 90 kDa binds FC are also presented. The evidence presented taken as a whole, suggests the occurrence in maize of a protein complex for FC perception and signal transduction.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aducci P, Ballio A and Federico R (1982) Solubilisation of fusicoccin binding sites, Plasmalemma and Tonoplast, Their Function in the Plant Cell. In: Marmè D, Maire E and Hertel R (eds), pp 279–284. Amsterdam: Elsevier
Aducci P, Ballio A, Fullone MR and Persichetti F (1986) Entrapment into liposomes of fusicoccin binding sites. Plant Sci 45: 83–86
Aducci P, Ballio A, Blein J-P, Fullone MR, Rossignol M and Scalla R (1988) Functional reconstitution of a proton-translocating system responsive to fusicoccin. Proc Natl Acad Sci USA 85: 7849–7851
Aducci P, Ballio A, Fogliano V, Fullone MR, Marra M and Proietti N (1993) Purification and photoaffinity labelling of fusicoccin receptors from com. Eur J Biochem 214: 339–345
Aitken A, Ellis CA, Harris A, Sellers LA and Toker A (1990) Kinase and neurotransmitters. Nature 334: 594
Aitken A, Collinge DB, van Heusden BPH, Isobe T, Roseboom PH, Rosenfeld G and Soll J (1992) 14-3-3 proteins: a highly conserved, widespread family of eucaryotic proteins. TIBS 17: 498–501
de Boer A, Watson BA and Cleland RE (1989) Purification and identification of the fusicoccin binding protein from oat root plasma membrane, Plant Physiol 89: 250–259
de Vetten NC, Lu G and Ferl RG (1992) A com protein associated with the G-box binding complex has homology to brain regulatory proteins. The Plant Cell 4: 1295–1307
Feyerabend M and Weiler EW (1989) Photoaffinity labelling and partial purification of the putative plant receptor for the fungal wilt-inducing toxin, fusicoccin. Planta 178: 282–290
Galardy RE, Craig LC, Jamieson GD and Printz MP (1974) Photoaffinity labelling of peptide hormone binding sites. J Biol Chem 249: 3510–3518
Glazer AN, Delange RJ and Sigman DS (1975) in Chemical modification of proteins. In: Work TS and Work E (eds), pp 167–179. Amsterdam: Elsevier Biomedical Press
Hirsch S, Aitken A, Bertsch U and Soil J (1992) A plant homologue to mammalian brain 14–3–3 protein and protein kinase C inhibitor. FEBS Lett 296: 222–224
Ichimura T, Isobe T, Okuyama T, Takahashi N, Araki K, Kuwano R and Takahashi Y (1988) Molecular cloning of cDNA coding for brain-specific kinase-dependent activator of tyrosine and tryptophan hydroxylases. Proc Natl Acad Sci USA 85: 7084–7088
Johansson F, Sommarin M and Larsson C (1993) Fusicoccin activates the plasma membrane H+ ATPase by a mechanism involving the C-terminal inhibitory domain. The Plant Cell 5: 321–327
Kidou S, Umeda M, Kato A and Uchimiya H (1993) Isolation and characterization of a rice cDNA similar to the bovine brain specific 14–3–3 protein gene. Plant Mol Biol 12: 191–194
Korthout HAAJ and de Boer AH (1994) A fusicoccin binding protein belongs to the family of 14–3–3 brain protein homologs. The Plant Cell 6: 1681–1692
Lu G, De Lisle A, de Vetten NC and Ferl R (1992) Brain protein in plants: an Arabidopsis homolog to neurotransmitter pathway activators is part of a DNA binding complex. Proc Natl Acad Sci 89: 11490–11494
Lu G, Sehnke PC and Ferl R (1994) Phosphorylation and calcium binding properties of an Arabidopsis GF14 brain protein homolog. The Plant Cell 6: 501–510
Marra M, Ballio A, Fullone MR and Aducci P (1992) Some properties of functional reconstituted plasmalemma H+ -ATPase activated by fusicoccin. Plant Physiol 98: 1029–1034
Marra M, Fullone MR, Fogliano V, Masi S, Mattei M, Pen J and Aducci, P (1994) The 30 kD protein present in purified fusicoccin receptor preparation is a 14–3–3-like protein. Plant Physiol 106: 1497–1501
Marré E (1979) Fusicoccin: a tool in plant physiology. Annu Rev Plant Physiol 30: 273–288
Mochly-Rosen D, Khaner H, Lopez J and Smith BL (1991) Intracellular receptor for activated protein kinase C. Identification of a binding site for the enzyme. J Biol Chem 266: 14866–14868
Oecking C and Weiler EW (1991) Characterization and purification of the fusicoccin binding complex from plasma membrane of Commelina communis. Eur J Biochem 199:685–689
Palmgren GM, Sommarin M, Serrano R and Larsson C (1991) Identification of an autoinhibitory domain in the C-terminal region of the plant plasma membrane H+-ATPase. J Biol Chem 266 (30): 20470–20475
Rasi-Caldogno F, De Michelis MI, Pugliarello MC and Marrè E (1986) H+-pumping driven by the plasma membrane ATPase in membrane vesicles from radish: stimulation by fusicoccin. Plant Physiol 82: 121–125
Rasi Caldogno F, Pugliarello MC, Olivari C and De Michelis MI (1993) Controlled proteolysis mimics the effect of FÇ on the plasma membrane H+-ATPase. Plant Physiol 103: 391– 398
Serrano R, Portillo F, Monk B and Palmgren GM (1992) The regulatory domain of fungal and plasma membrane H+-ATPase. Acta Physiol Scand 146: 131–146
Toker A, Sellers AL, Amess B, Patel Y, Harris A and Aitken A (1992) Multiple isoforms of a protein kinase C inhibitor (KCIP-1/14–3–3) from sheep brain. Eur J Biochem 206: 453–461
Vera-Estrella R, Barkla BJ, Higgins VJ and Blumwald E (1994) Plant defense response to fungal pathogen. Plant Physiol 104: 209–215
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Kluwer Academic Publishers
About this chapter
Cite this chapter
Aducci, P., Ballio, A., Nasta, D., Fogliano, V., Fullone, M.R., Marra, M. (1996). Fusicocein and its receptors. In: Smith, A.R., et al. Plant Hormone Signal Perception and Transduction. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0131-5_19
Download citation
DOI: https://doi.org/10.1007/978-94-009-0131-5_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6546-7
Online ISBN: 978-94-009-0131-5
eBook Packages: Springer Book Archive