Skip to main content

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 78))

Abstract

In our previous study, we isolated a high-affinity phosphate transporter gene, named PHT1, from Arabidopsis thaliana, and showed i) that expression of the gene at high levels in tobacco cultured cells increased the rate of phosphate uptake, and ii) that the transgenic cells exhibited increased biomass production when the supply of phosphate was limited (Mitsukawa et al., 1997 Proc. Natl. Acad. Sci. USA, 94, 7098–7102). In this study, two genes highly homologous to PHT1, named PHT2 and PHT3, were isolated from genomic DNA libraries. Nucleotide sequence comparison indicated conservation of the second intron sequence of PHT1 in PHT2. These three genes located closely on chromosome 5, indicating that they are not allelic with previously identified phosphate mutant loci pho1 and pho2, found on chromosome 2 and 3, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bun-ya M, Nishimura M, Harashima S and Oshima Y 1991 The PH084 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol. Cell. Biol. 11, 3229–3238.

    PubMed  CAS  Google Scholar 

  • Crawford N M 1994 Metabolic and genetic control of nitrate, phosphate, and iron assimilation in plants. In Arabidopsis. Eds. E M Meyerowitz and C R Somerville. pp 1119–1146. Cold Spring Harbor Laboratory Press, New York, USA.

    Google Scholar 

  • Delhaize E and Randall P J 1995 Characterization of a phosphate-accumulator mutant of Arabidopsis thaliana. Plant Physiol. 107, 207–213.

    PubMed  CAS  Google Scholar 

  • Harrison M J and van Buuren M L 1995 A phosphate transporter from the mycorrhizal fungus Glomus versiforme. Nature 378, 626–629.

    Article  PubMed  CAS  Google Scholar 

  • Jensen E O, Marcker K A, Schell J and de Bruijn F J 1988 Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghaemoglobin lbc3 5’upstream region. EMBO J. 7, 1265–1271.

    PubMed  CAS  Google Scholar 

  • Lessard P A, Allen R D, Bernier F, Crispino J D, Fujiwara T and Beachy R N 1991 Multiple nuclear factors interact with upstream sequences of differentially regulated β-conglycinin genes. Plant Mol. Biol. 16, 397–413.

    Article  PubMed  CAS  Google Scholar 

  • Lister C and Dean C 1993 Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana. Plant J. 4, 745–750.

    Article  CAS  Google Scholar 

  • Liu Y-G, Mitsukawa N, Vazques-Tello A and Whittier R F 1995 Generation of a high-quality PI library of Arabidopsis suitable for chromosome walking. Plant J. 7, 351–358.

    Article  CAS  Google Scholar 

  • Mitsukawa N, Okumura S, Shirano Y, Sato S, Kato T, Harashima S and Shibata D 1997 Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proc. Natl. Acad. Sci. USA 94, 7098–7102.

    Article  PubMed  CAS  Google Scholar 

  • Muchhal U S, Pardo J M and Raghothama K G 1996 Phosphate transporters from the higher plant Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 93, 10519–10523.

    Article  PubMed  CAS  Google Scholar 

  • Poirier Y, Thoma S, Somerville C and Schiefelbein J 1991 A mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiol. 97, 1087–1093.

    Article  PubMed  CAS  Google Scholar 

  • Saiki R K, Gelfand D H, Stoffel S, Scharf S J, Higuchi R, Horn G T, Mullis K B and Erlich H A 1988 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491.

    Article  PubMed  CAS  Google Scholar 

  • Smith F W, Ealing P M, Dong B and Delhaize E 1997 The cloning of two Arabidopsis genes belonging to a phosphate transporter family. Plant J. 11, 83–92.

    Article  PubMed  CAS  Google Scholar 

  • Versaw W K 1995 A phosphate-repressible, high-affinity phosphate permease is encoded by the pho-5 + gene of Neurospora crassa. Gene 153, 135–139.

    Article  PubMed  CAS  Google Scholar 

  • Wingender E, Kel A E, Kel O V, Karas H, Heinemeyer T, Dietze P, Knuppel R, Romaschenko A G and Kolchanov N A 1997 TRANSFAC, TRRD and COMPEL: towards a federated database system on transcriptional regulation. Nuc. Acids Res. 25, 265–268.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Daisuke Shibata .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Kluwer Academic Publishers

About this chapter

Cite this chapter

Mitsukawa, N., Okumura, S., Shibata, D. (1997). High-affinity phosphate transporter genes of Arabidopsis thaliana . In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_43

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-0047-9_43

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6510-8

  • Online ISBN: 978-94-009-0047-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics