Abstract
Fe absorption was examined using chloroplasts, isolated from leaves of hydroponically cultured Fe-sufficient barley plants, to clarify the mechanism underlying the light-dependent Fe-influx into the chloroplasts. Inhibitors of Fe transport, ATP and proton flux were employed. Fe transport into the chloroplasts was partially inhibited by bathophenanthrolinedisulfonic acid (BPDS), which suggested the involvement of Fe3+-reductase in the process of transmembrane Fe-influx. We also found that adenosine triphosphate (ATP) and proton (H+) flux were not the cause of transmembrane Fe transport. The optimum pH for Fe transport across the chloroplast envelope was 8.0. However, a sharp increase in the Fe absorption was observed in pH range of 7.0 to 7.5. Fe transport into the chloroplasts was unaffected by the presence of Zn, Cu, Ni, Mn, Co or Cd in the medium at 1 x 10-5 or 1 × 10-4 M concentration.
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© 1997 Kluwer Academic Publishers
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Bughio, N., Takahashi, M., Yoshimura, E., Nishizawa, N.K., Mori, S. (1997). Characteristics of light-regulated iron transport system in barley chloroplasts. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_33
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DOI: https://doi.org/10.1007/978-94-009-0047-9_33
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6510-8
Online ISBN: 978-94-009-0047-9
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