Abstract
In order to reveal the subcellular distribution and the exchange characteristics of Ca2+ in citrus (Citrus Unshiu Marc, on Poncirus trifoliata Raf.) root, Ca2+ concentrations in root subcellular compartments, unidirectional fluxes were analyzed using isotope exchange kinetics. The results showed that Ca2+ concentrations in free space, cytoplasm and vacuole were 33.135 µM, 1.029 µM and 20.157 µM. Unidirectional influx from environment to cytoplasm (ø oc), from cytoplasm to environment (ø co), from vacuole to cytoplasm (ø vc) and from cytoplasm to vacuole (ø cv) were 0.900 µmolg-1FWh-1, 0.803 µmolg-1 FW-1, 0.052 µmolg-1FWh-1 and 0.038 µ/molg-1 FWh-1 respectively. Some net fluxes and unidirectional rate-constants were also calculated. It was concluded that a low Ca2+ concentration in cytoplasm, a very low net influx and upward flux characterized the analysis. In addition, the modification of the calculation because of high Ca2+ content in free space was also discussed.
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References
Bell C I, Cram W J and Clarkson D T 1994 J Exp Bot. 45, 879–886
Cram W J 1988 In Advances in Plant Nutrition (Vol. 3). Ed. B Tinker and A Läuchli, ppl-53. Praeger Publishers, New York.
Epstein E 1972 Mineral Nutrition of Plants: Principles and Perspectives. John Wiley and Sons Ine, New York. 412p.
Jacquez J A 1985 Compartmental Analysis in Biology and Medicine. University of Michigan Press, Ann Arbor. 560p.
Kronzucker H J, Siddqi M Y and Glass A D M 1995 Plant Physiol. 109,481–490.
Thain J F 1984 J Exp. Bot. 35, 444–453.
Walker N A and Pitman MG 1976 In Encyclopedia of Plant Physiology, New Series, 2A. Ed. U Lüttage and M G Pitman, pp 36–52. Springer-Verlag, Berlin.
Wang MY Siddiqi MY, Ruth TJ and Glass ADM 1993 Plant Physiol. 103, 1249–1258.
Xie Shao-Ping and Ni Jin-Shang 1990 Acta Phytophysiologica Sinica. 16,271–276.
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© 1997 Kluwer Academic Publishers
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Xiao, L., Hu, D., Hong, Y., Zheng, Y. (1997). Compartmentation and flux analysis of Ca2+ in citrus root using isotope exchange kinetics. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_22
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DOI: https://doi.org/10.1007/978-94-009-0047-9_22
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