Abstract
The isolation of DNA from small quantities of plant material has been the subject of a large number of recent articles (1,2,3). The methods described to date may be divided into protocols which either produce DNA of sufficient quality and quantity to be analysed immediately or which produce small quantities of DNA that can be analysed only following amplification by the polymerase chain reaction. Within the first category, only the ‘scaled down’ CTAB extraction procedure, as described in protocol 1, can produce, on a routine basis, sufficient good quality DNA, from a variety of starting material, which is suitable for most purposes. Careful use of this procedure may produce up to 25 µg of restrictable DNA from as little as 0.05 g of freeze-dried tissue.
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References
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© 1998 Chapman & Hall
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Edwards, K.J. (1998). Miniprep Procedures for the Isolation of Plant DNA. In: Karp, A., Isaac, P.G., Ingram, D.S. (eds) Molecular Tools for Screening Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0019-6_5
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DOI: https://doi.org/10.1007/978-94-009-0019-6_5
Publisher Name: Springer, Dordrecht
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