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Hexanoyl-Lysine as an Oxidative-Injured Marker – Application of Development of Functional Food

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Book cover Lipid Hydroperoxide-Derived Modification of Biomolecules

Part of the book series: Subcellular Biochemistry ((SCBI,volume 77))

Abstract

We could proposed that Nε-(hexanoy)lysine, HEL, become a useful biomarker for detection of oxidative stress damage occurred by exhaustive exercise. We examined the preventive effect of flavonoid compound, eriocitrin, against exercise-induced oxidative damage in rat liver. Eriocitrin administration prior to exercise significantly suppressed the increases in thiobarbituric acid-reactive substance caused by lipid peroxidation during exhaustive exercise. The increase in the contents of HEL in rat liver was also abolished by eriocitrin administration. The concentration of oxidized glutathione was significantly increased by exercise, but the eriocitrin administration suppressed this increase. These results suggested that eriocitrin administration prior to exercise prevented oxidative damages caused by exhaustive exercise-induced oxidative stress. Therefore, it was suggested that HEL could be a good biomarker for oxidative stress, especially at earlier stage when oxidative damage was occurred by lipid peroxidation than a stage of harmful aldehyde formation. Moreover, it was suggested that eriocitrin metabolites, eriodictyol and 3, 4 – dihydroxyhydrocinnamic, might scavenge free radicals and reactive oxygen species, resulting in suppression of lipid peroxidation and reactive proteins with radicals to form HEL. These findings implied that eriocitrin might be useful as an anti-oxidative compound to protect oxidative stress damages.

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Abbreviations

HEL:

Nε-(hexanonyl)lysine

DT:

o,o-dityrosine

NT:

Nitrotyrosine

TBARS:

Thiobarbituric acid-reactive substance

ROS:

Reactive oxygen species

ELISA:

Enzyme-linked immunosorbent assay

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

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Acknowledgment

This work was supported by a grant aid from the Japan Science and Technology Corporation (JST). We are deeply grateful to Professor Toshihiko Osawa of Aichi- Gakuin University for his guidance.

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Meijo University, 1-501 Shiogamaguchi, Nagoya, 468-8502, Japan

    Ken-ichiro Minato

  2. Department of Human Wellness, Tokaigakuen University, 21-233, Nishinohora, Ukigai-cho, Miyoshi, Aichi, 470-0207, Japan

    Yoshiaki Miyake

Authors
  1. Ken-ichiro Minato
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  2. Yoshiaki Miyake
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Corresponding author

Correspondence to Ken-ichiro Minato .

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Editors and Affiliations

  1. School of Human Science & Environment, University of Hyogo, Himeji, Hyogo, Japan

    Yoji Kato

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Minato, Ki., Miyake, Y. (2014). Hexanoyl-Lysine as an Oxidative-Injured Marker – Application of Development of Functional Food. In: Kato, Y. (eds) Lipid Hydroperoxide-Derived Modification of Biomolecules. Subcellular Biochemistry, vol 77. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7920-4_14

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