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Trypanosoma cruzi Trans-Sialidase: Structural Features and Biological Implications

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Proteins and Proteomics of Leishmania and Trypanosoma

Part of the book series: Subcellular Biochemistry ((SCBI,volume 74))

Abstract

Trypanosoma cruzi trans-sialidase (TcTS) has intrigued researchers all over the world since it was shown that T. cruzi incorporates sialic acid through a mechanism independent of sialyltransferases. The enzyme has being involved in a vast myriad of functions in the biology of the parasite and in the pathology of Chagas’ disease. At the structural level experiments trapping the intermediate with fluorosugars followed by peptide mapping, X-ray crystallography, molecular modeling and magnetic nuclear resonance have opened up a three-dimensional understanding of the way this enzyme works. Herein we review the multiple biological roles of TcTS and the structural studies that are slowly revealing the secrets underlining an efficient sugar transfer activity rather than simple hydrolysis by TcTS.

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Abbreviations

4-MUNeu5Ac:

4-methylumbelliferyl-N-acetyl neuraminic acid

Galf :

Galactofuranose

Galp :

Galactopyranose

GlcNAc:

N-acetylglucosamine

Neu5Ac:

N-acetylneuraminic

Neu5Gc:

N-glycolylneuraminic acid

NGF:

Nerve growth factor receptor

pNPNeu5Ac:

p-nitrophenyl-N-acetyl-neuraminic acid

SAPA:

Shed acute phase antigen

Sias:

Sialic acids

Siglecs:

Sia-binding Ig-like lectin

TcTS:

Trypanosoma cruzi trans-sialidase

TSs:

Trans-sialidase family

UTR:

Untranslated regions

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Acknowledgments

The study was financially supported by Conselho Nacional de Desenvolvimento e Tecnologia (CNPq) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and the National Institute for Science and Technology in Vaccines (CNPq 573547/2008-4).

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  1. Laboratório de Glicobiologia Estrutural e Funcional, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Centro de Ciências da Saúde-Bloco D-3, 21941-902, Cidade Universitária, Rio de Janeiro, Brazil

    Isadora A. Oliveira, Leonardo Freire-de-Lima, Luciana L. Penha, Wagner B. Dias & Adriane R. Todeschini

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  1. Isadora A. Oliveira
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  2. Leonardo Freire-de-Lima
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  3. Luciana L. Penha
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  4. Wagner B. Dias
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  5. Adriane R. Todeschini
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Correspondence to Adriane R. Todeschini .

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Editors and Affiliations

  1. Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    André L.S. Santos

  2. Departamento de Microbiologia Geral, Ins. de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

    Marta H. Branquinha

  3. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Claudia M. d’Avila-Levy

  4. Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Lucimar F. Kneipp

  5. Departamento de Biologia Celular e Molecular, Instituto de Biologia Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Cátia L. Sodré

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Oliveira, I.A., Freire-de-Lima, L., Penha, L.L., Dias, W.B., Todeschini, A.R. (2014). Trypanosoma cruzi Trans-Sialidase: Structural Features and Biological Implications. In: Santos, A., Branquinha, M., d’Avila-Levy, C., Kneipp, L., Sodré, C. (eds) Proteins and Proteomics of Leishmania and Trypanosoma. Subcellular Biochemistry, vol 74. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7305-9_8

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