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Bioprocessing of Human Pluripotent Stem Cells for Cell Therapy Applications

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Stem Cells and Cell Therapy

Part of the book series: Cell Engineering ((CEEN,volume 8))

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Abstract

Human pluripotent stem cells (hPSCs), with their unique characteristics for indefinite proliferation and pluripotency, are an appealing source for cell replacement therapies, tissue engineering, drug discovery and in vitro toxicology. For the clinical implementation of these cells, there is the need for translating the culture protocols developed at research laboratories into validated bioprocesses that can guarantee reproducibility, scalability, standardization, robustness and safety.

The most attractive strategy for hPSC manufacturing consists in engineering stem cell niches by identifying key factors governing hPSC cell fate and creating culturing approaches that allow for 3D cell organization in a bioreactor-based system where the key environmental conditions are finely controlled. This chapter provides an overview of current bioengineering strategies that could be used to generate large numbers of hPSCs and/or their derivatives with potential application in regenerative medicine and drug discovery.

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Acknowledgments

The authors would like to acknowledge the financial support received from the Portuguese Foundation for Science and Technology (PTDC/BIO/72755/2006) and from the European Commission (Cell Programming by Nanoscaled Devices, NMP4-CT-2004-500039; Clinigene Network of Excellence, LSHB-CT-2006-018933; HYPERLAB - high yield and performance stem cell lab, 223011).

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  1. Animal Cell Technology Unit, Instituto de Biologia Experimental e Tecnológica (IBET), Apartado 12, Oeiras, 2780-901, Portugal

    Margarida Serra, Cláudia Correia, Catarina Brito & Paula M. Alves

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  1. Margarida Serra
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  2. Cláudia Correia
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  3. Catarina Brito
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  4. Paula M. Alves
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Correspondence to Paula M. Alves .

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Editors and Affiliations

  1. Engineering, University College, Dublin Dept. Chemical & Biochemical, Belfield, Dublin, Ireland

    Mohamed Al-Rubeai

  2. School of Chem. & Bioprocess Eng., University College Dublin, Dublin, Dublin, Ireland

    Mariam Naciri

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Serra, M., Correia, C., Brito, C., Alves, P.M. (2014). Bioprocessing of Human Pluripotent Stem Cells for Cell Therapy Applications. In: Al-Rubeai, M., Naciri, M. (eds) Stem Cells and Cell Therapy. Cell Engineering, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7196-3_4

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