Abstract
Originally, work on the biosynthesis of protochlorophyll(ide) [Pchl(ide)] and chlorophyll (Chl) in organello, started in 1967 in my laboratory at the National Research Institute in Tel-el-Amara, Lebanon (see Chap. 2) (Rebeiz 1967, 1968). At the time spectrophotometric instrumentation was used. Since I was aware that excised etiolated cucumber cotyledons greened very rapidly, within hours, in the light, I conjectured that if greening cotyledons were homogenized, I should be able to observe Pchl(ide) and Chl formation in the homogenate for a few minutes before the system fell apart. The first evidence of Chl biosynthesis in organello was observed in 1967 (Rebeiz 1967). However I soon realized that spectrophotometric techniques were not sensitive enough to observe consistent and reliable Pchl(ide) and Chl biosynthesis in organello. I therefore shifted to the use of 14C-δ-aminolevulinic acid (14C-ALA) as a precursor of 14C-Chl. At the time ALA was known as a tetrapyrrole precursor (Granick 1961). We observed the first incorporation of 14C-ALA into 14C-Chl in my laboratory in 1969. The work was perfected in California at UC Davis in 196–1970 when I joined Paul’s Castelfranco Laboratory (Rebeiz and Castelfranco 1971a, b).
All truths are easy to understand once they are discovered. The point is to discover them (Galileo Galilei).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abd-El-Mageed HA, El Sahhar KF, Robertson KR et al (1997) Chloroplast biogenesis 77. Two novel monovinyl and divinyl light–dark greening groups of plants and their relationship to the chlorophyll a biosynthetic heterogeneity of green plants. Photochem Photobiol 66:89–96
Bazzaz MB, Rebeiz CA (1979) Chloroplast culture V. Spectrofluorometric determination of chlorophyll(ide) a and b and pheophytin (or pheophorbide) a and b in unsegregated pigment mixtures. Photochem Photobiol 30:709–721
Beale SI, Castelfranco PA (1974) The biosynthesis of δ-aminolevulinic acid in higher plants. II. Formation of 14C-δ-aminolevulinic acid from labelled precursors in greening plant tissues. Plant Physiol 53:291–296
Daniell H, Rebeiz CA (1982a) Chloroplast culture VIII. A new effect of kinetin in enhancing the synthesis and accumulation of protochlorophyllide in vitro. Biochem Biophys Res Commun 104:837–843
Daniell H, Rebeiz CA (1982b) Chloroplast culture IX. Chlorophyll(ide) a biosynthesis in vitro at rates higher than in vivo. Biochem Biophys Res Commun 106:466–470
Daniell H, Rebeiz CA (1984) Bioengineering of photosynthetic membranes: requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro. Biotechnol Bioeng 26:481–487
Duggan JX, Rebeiz CA (1982a) Chloroplast biogenesis 37: induction of chlorophyllide a (E459F675) accumulation in higher plants. Plant Sci Lett 24:27–37
Duggan JX, Rebeiz CA (1982b) Chloroplast biogenesis 42. Conversion of DV chlorophyllide a to monovinyl chlorophyllide a in vivo and in vitro. Plant Sci Lett 27:137–145
Ellsworth RK, Aronoff S (1968) Investigations on the biogenesis of chlorophyll a. III. Biosynthesis of Mg-vinylporphine a 5 methylester from Mg-protoporphine IX monomethyl ester as observed in Chlorella mutants. Arch Biochem Biophys 125:269–277
Ellsworth RK, Hsing AS (1973) The reduction of vinyl-side chains of Mg-protoporphyrin IX monomethyl ester in vitro. Photosynthetica 313:119–129
Fuesler TP, Wright LA, Castelfranco PA (1981) Properties of magnesium chelatase in greening etioplasts. Metal ion specificity and effect of substrate concentration. Plant Physiol 67:246–249
Fuesler TP, Castelfranco PA, Wong YS (1984a) Formation of Mg-containing chlorophyll precursors from protoporphyrin IX, d-aminolevulinic acid, and glutamate in isolated, photo synthetically competent, developing chloroplasts. Plant Physiol 74:928–933
Fuesler TP, Wong YS, Castelfranco PA (1984b) Localization of Mg-chelatase and Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase activities within isolated, developing chloroplasts. Plant Physiol 75:662–664
Granick S (1961) Magnesium protoporphyrin monoester and protoporphyrin monomethyl ester in chlorophyll biosynthesis. J Biol Chem 236:1168–1172
Ioannides IM, Fasoula DM, Robertson KR et al (1994) An evolutionary study of chlorophyll biosynthetic heterogeneity in green plants. Biochem Syst Ecol 22:211–220
Jeffrey SW, Wright SW (1987) A new spectrally distinct component in preparations of chlorophyll c from micro-alga (Prymnesiophyceae). Biochim Biophys Acta 894:180–188
Kim JS, Kolossov V, Rebeiz CA (1997) Chloroplast biogenesis 76: regulation of 4-vinyl reduction during conversion of divinyl Mg-protoporphyrin IX to monovinyl protochlorophyllide a is controlled by plastid membrane and stromal factors. Photosynthetica 34:569–581
Kolossov VL, Rebeiz CA (2001) Chloroplast biogenesis 84. Solubilization and partial purification of membrane-bound [4-vinyl] chlorophyllide a reductase from etiolated barley leaves. Anal Biochem 295:214–219
Kolossov VL, Rebeiz CA (2010) Evidence for Various 4-vinyl reductase activities in higher plants. In: Rebeiz CA, Benning C, Bohnert HJ et al (eds) The chloroplast: basics and applications. Springer, Netherlands, pp 25–38
Lee HJ, Ball M, Rebeiz CA (1991) Intraplastidic localization of the enzymes that convert delta-aminolevulinic acid to protoporphyrin IX in etiolated cucumber cotyledons. Plant Physiol 96:910–915
Lee HJ, Ball MD, Parham R et al (1992) Chloroplast biogenesis 65. Enzymic conversion of protoporphyrin IX to Mg-protoporphyrin IX in a subplastidic membrane fraction of cucumber etiochloroplasts. Plant Physiol 99:1134–1140
Mattheis JR, Rebeiz CA (1977a) Chloroplast biogenesis. Net synthesis of protochlorophyllide from protoporphyrin IX by developing chloroplasts. J Biol Chem 252:8347–8349
Mattheis JR, Rebeiz CA (1977b) Chloroplast biogenesis. Net synthesis of protochlorophyllide from magnesium protoporphyrin monoester by developing chloroplasts. J Biol Chem 252:4022–4024
Mauzerall D, Granick S (1956) The occurrence and determination of delta-aminolevulinic acid and porphobilinogen in urine. J Biol Chem 219:435–446
Pardo AD, Chereskin BM, Castelfranco PA et al (1980) ATP requirement for Mg chelatase in developing chloroplasts. Plant Physiol 65:956–960
Parham R, Rebeiz CA (1992) Chloroplast biogenesis: [4-vinyl] chlorophyllide a reductase is a divinyl chlorophyllide a-specific NADPH-dependent enzyme. Biochemistry 31:8460–8464
Parham R, Rebeiz CA (1995) Chloroplast biogenesis 72: a [4-vinyl] chlorophyllide a reductase assay using divinyl chlorophyllide a as an exogenous substrate. Anal Biochem 231:164–169
Perkins HJ, Roberts DWA (1960) Chlorophyll biosynthesis in wheat leaves. Biochem Biophys Acta 45:613–620
Perkins EJ, Roberts DWA (1962) Purification of chlorophylls, pheophytins, an pheophorbides for specific activity determinations. Biochim Biophys Acta 58:486–498
Rebeiz CA (1967) Studies on chlorophyll biosynthesis in etiolated excised cotyledons of germinating cucumber at different stages of seedling development. Magon Serie Scientifique 13:1–21
Rebeiz CA (1968) The chloroplast pigments of etiolated and greening cucumber cotyledons. Magon Serie Scientifique 21:1–25
Rebeiz CA, Castelfranco P (1971a) Protochlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol 47:24–32
Rebeiz CA, Castelfranco P (1971b) Chlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol 47:33–37
Rebeiz CA, Yaghi M, Abou Haidar M et al (1970) Protochlorophyll biosynthesis in cucumber (Cucumis sativus, L.) cotyledons. Plant Physiol 46:57–63
Rebeiz CA, Larson S, Weier TE et al (1973) Chloroplast maintenance and partial differentiation in vitro. Plant Physiol 51:651–659
Rebeiz CA, Mattheis JR, Smith BB et al (1975a) Chloroplast biogenesis. Biosynthesis and accumulation of protochlorophyll by isolated etioplasts and developing chloroplasts. Arch Biochem Biophys 171:549–567
Rebeiz CA, Mattheis JR, Smith BB et al (1975b) Chloroplast biogenesis. Biosynthesis and accumulation of Mg-protoporphyrin IX monoester and longer wavelength metalloporphyrins by greening cotyledons. Arch Biochem Biophys, 166:446–465
Rebeiz CA, Daniell H, Mattheis JR (1982) Chloroplast bioengineering: the greening of chloroplasts in vitro. In: Scott CD (ed) Biotechnology bioengineering symposium, vol 12, John Wiley, New York, pp 414–439
Rebeiz CA, Montazer-Zouhoor A, Daniell H (1984) Chloroplast culture X: thylakoid assembly in vitro. Isr J Bot 33:225–235
Rebeiz CA, Kolossov VL, Briskin D et al (2003) Chloroplast biogenesis: chlorophyll biosynthetic heterogeneity, multiple biosynthetic routes and biological spin-offs. In: Nalwa HS (ed) Handbook of photochemistry and photobiology, vol 4. American Scientific Publishers, Los Angeles, pp 183–248
Scopes RK (1982) Protein purification; principles and practice. Springer, New York, pp 185–200
Shedbalkar VP, Rebeiz CA (1992) Chloroplast biogenesis: determination of the molar extinction coefficients of divinyl chlorophyll a and b and their pheophytins. Anal Biochem 207:261–266
Sisler EC, Klein W (1963) The effect of age and various chemicals on the lag phase of chlorophyll synthesis in dark grown bean seedlings. Physiol Plant 16:315–322
Smith BB, Rebeiz CA (1977a) Chloroplast biogenesis: detection of Mg-protoporphyrin chelatase in vitro. Arch Biochem Biophys 180:178–185
Smith BB, Rebeiz CA (1977b) Spectrofluorometric determination of Mg-protoporphyrin monoester and longer wavelength metalloporphyrins in the presence of Zn-protoporphyrin. Photochem Photobiol 26:527–532
Smith BB, Rebeiz CA (1979) Chloroplst biogenesis XXIV. Intrachloroplastic localization of the biosynthesis and accumulation of protoporphyrin IX, magnesium protoporphyrin IX, Magnesium-protoporphyrin monoester and longer wavelength metalloporphyrins during greening. Plant Physiol 63:227–231
Stetler DA, Laetsch WM (1965) Kinetin-induced chloroplast maturation in cultures of tobacco tissue. Science 149:1387–1388
Tripathy BC, Rebeiz CA (1988) Chloroplast biogenesis 60. Conversion of divinyl protochlorophyllide to monovinyl protochlorophyllide in green(ing) barley, a dark monovinyl/light divinyl plant species. Plant Physiol 87:89–94
Walker CJ, Weinstein JD (1991a) In vitro assay of the chlorophyll biosynthetic enzyme Mg- chelatase: resolution of the activity into soluble and membrane-bound fractions. Proc Natl Acad Sci U S A 88:5789–5793
Walker CJ, Weinstein JD (1991b) Further characterization of the magnesium chelatasein isolated developing cucumber chloroplasts. Plant Physiol 95:1189–1196
Wickliff JL, Aronoff S (1963) Turnover of chlorophyll a in mature soybean leaves. Plant and Cell Physiol:441–449
Wu SM, Mayasich JM, Rebeiz CA (1989) Chloroplast biogenesis: quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry. Anal Biochem 178:294–300
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Rebeiz, C.A. (2014). Development of Cell-Free Systems. In: Chlorophyll Biosynthesis and Technological Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7134-5_4
Download citation
DOI: https://doi.org/10.1007/978-94-007-7134-5_4
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-7133-8
Online ISBN: 978-94-007-7134-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)