Abstract
The successful investigations of the chlorophyll biosynthetic pathway would not have been possible without the development of new analytical and preparatory techniques. These techniques form the object of this chapter.
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References
Bazzaz MB, Rebeiz CA (1978) Chloroplast culture: the chlorophyll repair potential of mature chloroplasts incubated in a simple medium. Biochim Biophys Acta 504:310–323
Bazzaz MB, Rebeiz CA (1979) Chloroplast culture V. Spectrofluorometric determination of chlorophyll(ide) a and b and pheophytin (or pheophorbide) a and b in unsegregated pigment mixtures. Photochem Photobiol 30:709–721
Belanger FC, Rebeiz CA (1982) Chloroplast biogenesis: detection of monovinyl magnesium protoporphyrin monoester and other monovinyl magnesium porphyrins in higher plants. J Biol Chem 257:1360–1371
Belanger FC, Rebeiz CA (1984) Chloroplast biogenesis 47: spectroscopic study of net spectral shifts induced by ligand coordination in metalated tetrapyrroles. Spectrochim Acta 40A:807–827
Belanger FC, Dugan JX, Rebeiz CA (1982) Chloroplast biogenesis: identification of chlorophyllide a (E458F674) as a divinyl chlorophyllide a. J Biol Chem 257:4849–4858
Daniell H, Rebeiz CA (1984) Bioengineering of photosynthetic membranes: requirement of magnesium for the conversion of chlorophyllide a to chlorophyll a during the greening of etiochloroplasts in vitro. Biotechnol Bioeng 26:481–487
Granick S (1948) Protoporphyrin 9 as a precursor of chlorophyll. J Biol Chem 172:717–727
Ioannides IM, Shedbalkar VP, Rebeiz CA (1997) Quantitative determination of 2-monovinyl protochlorophyll(ide) b by spectrofluorometry. Anal Biochem 249:241–244
Rebeiz CA, Belanger FC (1984) Chloroplast biogenesis 46: calculation of net spectral shifts induced by axial ligand coordination in metalated tetrapyrroles. Spectrochim Acta 40A:793–806
Rebeiz CA, Castelfranco P (1971a) Protochlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol 47:24–32
Rebeiz CA, Castelfranco P (1971b) Chlorophyll biosynthesis in a cell-free system from higher plants. Plant Physiol 47:33–37
Rebeiz CA, Lascelles J (1982) Biosynthesis of pigments in plants and bacteria. In: Govindgee (ed) Photosynthesis: energy conversion by plants and bacteria, vol 1. Academic, New York, pp 699–780
Rebeiz CA, Mattheis JR, Smith BB et al (1975a) Chloroplast biogenesis. Biosynthesis and accumulation of protochlorophyll by isolated etioplasts and developing chloroplasts. Arch Biochem Biophys 171:549–567
Rebeiz CA, Mattheis JR, Smith BB et al (1975b) Chloroplast biogenesis. Biosynthesis and accumulation of Mg-protoporphyrin IX monoester and longer wavelength metalloporphyrins by greening cotyledons. Arch Biochem Biophys 166:446–465
Rebeiz CA, Montazer-Zouhoor A, Daniell H (1984a) Chloroplast culture X: thylakoid assembly in vitro. Isr J Bot 33:225–235
Rebeiz CA, Montazer-Zouhoor A, Hopen HJ et al (1984b) Photodynamic herbicides: 1. Concept and phenomenology. Enzyme Microbiol Technol 6:390–401
Rebeiz CA, Tripathy BC, Mayasich JM (1988) Chloroplast biogenesis 61: kinetic analysis of precursor-product relationships in complex biosynthetic pathways. J Theor Biol 133:319–326
Shedbalkar VP, Ioannides IM, Rebeiz CA (1991) Chloroplast biogenesis. Detection of monovinyl protochlorophyll(ide) b in plants. J Biol Chem 266:17151–17157
Smith BB, Rebeiz CA (1977a) Spectrofluorometric determination of Mg-protoporphyrin monoester and longer wavelength metalloporphyrins in the presence of Zn-protoporphyrin. Photochem Photobiol 26:527–532
Smith BB, Rebeiz CA (1977b) Chloroplast biogenesis: detection of Mg-protoporphyrin chelatase in vitro. Arch Biochem Biophys 180:178–185
Thornber JP, Gregory RPF, Smith CA et al (1967) Studies on the nature of the chloroplast lamella. I. Preparation and some properties of two chlorophyll-protein complexes. Biochemistry 6:391–396
Tripathy BC, Rebeiz CA (1985) Chloroplast biogenesis. Quantitative determination of monovinyl and divinyl Mg-protoporphyrins and protochlorophyll(ides) by spectrofluorometry. Anal Biochem 149:43–61
Tripathy BC, Rebeiz CA (1988) Chloroplast biogenesis 60. Conversion of divinyl protochlorophyllide to monovinyl protochlorophyllide in green(ing) barley, a dark monovinyl/light divinyl plant species. Plant Physiol 87:89–94
Wu SM, Mayasich JM, Rebeiz CA (1989) Chloroplast biogenesis: quantitative determination of monovinyl and divinyl chlorophyll(ide) a and b by spectrofluorometry. Anal Biochem 178:294–300
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Rebeiz, C.A. (2014). Development of Analytical and Preparatory Techniques. In: Chlorophyll Biosynthesis and Technological Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7134-5_3
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DOI: https://doi.org/10.1007/978-94-007-7134-5_3
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