Abstract
Nuclear magnetic resonance (NMR) has transformed the research areas of Chemistry, Biochemistry and Medicine, but much of its fundamentals remain obscure for the non-initiated. NMR is a technique based on the absorption of radiofrequency radiation by atomic nuclei in the presence of an external magnetic field. In this chapter, we describe the physical basis of phenomena within NMR spectroscopy from both a quantum-theory perspective and a classical view, to provide any prospective user the basic concepts underlying the technique. The spectroscopic notion of energy level population is described, as well as a basic introduction to the theory and mechanisms of spin relaxation. The application of radiofrequency pulses to produce the NMR signal, its conversion to the frequency domain by Fourier Transform and the typical instrumental set-up of magnetic resonance are also covered.
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Barjat H, Chilvers PB, Fetler BK, Horne TJ, Morris GA (1997) A practical method for automated shimming with normal spectrometer hardware. J Magn Reson 125:197–201
Berger S, Braun S (2003) 200 and more NMR experiments. Wiley-VCH, New York
Bracewell RM (1978) The Fourier transform and its applications, 2nd edn. McGraw Hill, New York
Cavanagh J, Fairbrother WJ, Palmer AG 3rd, Skelton NJ (1996) Protein NMR spectroscopy. Principles and practice, 1st edn. Academic Press, New York
Claridge TDW (1999) High-resolution NMR techniques in organic chemistry. Pergamon Press, Oxford
Derome AE (1987) Modern NMR techniques for chemistry research. Pergamon Press, Oxford
Ernst RR, Bodenhausen G, Wokau A (1987) Principles of NMR in one or two dimensions. Clarendon Press, Oxford
Evans JNS (1996) Biomolecular NMR spectroscopy. Oxford University Press, Oxford
Farrar TC, Becker ED (1971) Pulse and Fourier transform NMR. Academic Press, New York
Günther H (1995) NMR spectroscopy: basic principles, concepts and applications in chemistry, 2nd edn. Wiley, Chichester
Harris RK, Mann BE (1978) NMR and the periodic table. Academic Press, London
Hurd RE (1990) Gradient enhanced spectroscopy. J Magn Reson 87:422–428
Keeler J (2006) Understanding NMR spectroscopy, 2nd edn. Wiley, Chichester
Keeler J, Clowes RT, Davis AL, Laue ED (1994) Pulse-field gradients: theory and practice. Methods Enzymol 239:145–207
Mason J (1987) Multinuclear NMR. Plenum, New York
Sanders JKM, Hunter BK (1992) Modern NMR spectroscopy, 2nd edn. Oxford University Press, Oxford
Vanzijl PCM, Sukumar S, Johnson MO, Webb P, Hurd RE (1994) Optimized shimming for high resolution NMR using three-dimensional image-based field mapping. J Magn Reson 111:203–207
Wüthrich K (1986) NMR of proteins and nucleic acids. Wiley, New York
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Carbajo, R.J., Neira, J.L. (2013). The Basis of Nuclear Magnetic Resonance Spectroscopy. In: NMR for Chemists and Biologists. SpringerBriefs in Biochemistry and Molecular Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6976-2_1
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DOI: https://doi.org/10.1007/978-94-007-6976-2_1
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