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Wine Components Normalize the Cytochrome P450 Content in the Liver and Kidneys of Rats Under Neurogenic Stress

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Advanced Bioactive Compounds Countering the Effects of Radiological, Chemical and Biological Agents

Abstract

A majority of organic environmental contaminants need to undergo the metabolic activation to exert their biological effects (toxic, mutagenic, transforming, etc.). This activation is catalyzed by monooxygenase system, which functional element is cytochrome P450 superfamily. One of the important properties of monooxygenase system is the ability of induction under the influence of external stimuli. Xenobiotics, different physiological factors, and stress can act as these stimuli. In our work, we studied cytochrome P450 content in the organs of the experimental animals under acute neurogenic stress and under the influence of alcohol and grape polyphenols. The obtained results showed that stressed animals had the low liver cytochrome P450 content compared with intact animals. Ethanol administration caused increasing of cytochrome P450 content in the liver of intact and stressed rats. Polyphenolic concentrate Enoant normalized the cytochrome P450 content in liver, whereas grape wines Cabernet and Rkatsiteli significantly increased cytochrome P450 content in both intact and stressed animals. The prophylactic administration of polyphenol concentrates to control animals did not significantly influence the cytochrome P450 content in hepatocytes and in rat kidneys. The cytochrome P450 content significantly decreased in animal kidneys under emotional-pain stress, but this effect was attenuated by ethanol administration. Incubation of isolated hepatocytes in the presence of phenacetin was accompanied by increased levels of cytochrome P450 in the cells. Grape polyphenols reduced cytochrome P450 content in the cells in dose-dependent and environment (e.g. stress, ethanol administration)-dependent manners, which suggests their possible role in the cancer prevention.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, The National University of Pharmacy, 53 Pushkinska St., Kharkiv, 61002, Ukraine

    Andrey Zagayko, Oksana Krasilnikova & Anna Kravchenko

Authors
  1. Andrey Zagayko
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  2. Oksana Krasilnikova
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  3. Anna Kravchenko
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Corresponding author

Correspondence to Andrey Zagayko .

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Editors and Affiliations

  1. St. Boniface Hospital Research Centre, Tache Ave 351, Winnipeg, R2H 2A6, Canada

    Grant N. Pierce

  2. , Division of Health and Rehabilitation, Crimean State Humanitarian University, Sevastopolskaya St. 2, Yalta, Crimea, 98635, Ukraine

    Volodymyr I. Mizin

  3. St. Boniface Hospital Research Centre, Tache Avenue 351, Winnipeg, R2H 2A6, Manitoba, Canada

    Alexander Omelchenko

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Zagayko, A., Krasilnikova, O., Kravchenko, A. (2013). Wine Components Normalize the Cytochrome P450 Content in the Liver and Kidneys of Rats Under Neurogenic Stress. In: Pierce, G., Mizin, V., Omelchenko, A. (eds) Advanced Bioactive Compounds Countering the Effects of Radiological, Chemical and Biological Agents. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6513-9_25

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