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Coherent Diffraction and Holographic Imaging of Individual Biomolecules Using Low-Energy Electrons

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Advancing Methods for Biomolecular Crystallography

Abstract

Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50–250 eV offer a possibility of overcome the problem of radiation damage, and obtaining images of individual biomolecules. Two experimental schemes for obtaining images of individual molecules – holography and coherent diffraction imaging – are discussed and compared. Images of individual molecules obtained by both techniques, using low-energy electrons, are shown.

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Acknowledgments

We would like to thank the Swiss National Science Foundation for its financial support.

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Authors and Affiliations

  1. Physics Institute, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland

    Tatiana Latychevskaia, Jean-Nicolas Longchamp, Conrad Escher & Hans-Werner Fink

Authors
  1. Tatiana Latychevskaia
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  2. Jean-Nicolas Longchamp
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  3. Conrad Escher
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  4. Hans-Werner Fink
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Corresponding author

Correspondence to Tatiana Latychevskaia .

Editor information

Editors and Affiliations

  1. , Department of Haematology, University of Cambridge, Hills Road, Cambridge, CB2 0XY, United Kingdom

    Randy Read

  2. Fac.des Sciences et des Technologies, Physics Department, Université de Lorraine, Blvd. des Aiguillettes 1, Vandoeuvre-lès-Nancy, 54506, France

    Alexandre G. Urzhumtsev

  3. , Institute of Mathematical Problems of Bi, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, 142290, Russia

    Vladimir Y. Lunin

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Latychevskaia, T., Longchamp, JN., Escher, C., Fink, HW. (2013). Coherent Diffraction and Holographic Imaging of Individual Biomolecules Using Low-Energy Electrons. In: Read, R., Urzhumtsev, A., Lunin, V. (eds) Advancing Methods for Biomolecular Crystallography. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6232-9_29

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