Abstract
Determination in the past and the contemporary re-determination of several protein structures are considered in the context of our personal experience with the use of single-wavelength anomalous dispersion (SAD) techniques. A unifying feature of these cases is that in each event, the anomalous signal generated by metal ions was not optimized to the peak of the absorption edge, but was, nevertheless sufficient for structure re-determination. Examples are described with the emphasis on educational aspects. Determinants of success are discussed in each case. In addition, briefly discussed are some concepts related to the quality of the diffraction data used in experimental phasing, with special attention to SAD phasing.
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Frolow, F. (2013). Use of a Weak Anomalous Signal for Phasing in Protein Crystallography: Reflection from Personal Experience. In: Read, R., Urzhumtsev, A., Lunin, V. (eds) Advancing Methods for Biomolecular Crystallography. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6232-9_16
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DOI: https://doi.org/10.1007/978-94-007-6232-9_16
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