Abstract
The potential application of thermophiles and their enzymes for industry is enormous where the development of host-vector systems in thermophiles is essential. Compared to mesophilic organisms, the host-vector systems in thermophiles are lagging behind. However, in recent years a number of host-vector systems were developed for thermophilic bacteria and also for some archaea. Host-vector systems in thermophiles are more advanced than is commonly believed.
In this chapter, firstly, we introduce basic genetic methodology for host-vector systems in thermophiles including transformation, selectable markers, vectors, and hosts (recipient cells). We show transformation methods used for thermophiles, selectable markers that are effective for thermophiles, some Escherichia coli-host shuttle vectors, and important property of the host.
Secondly, we show the actual application systems for the host-vector systems in thermophiles, expression vectors, reporter gene systems, and targeted gene disruption (replacement) method.
Finally, we introduce some commercial and potential application of thermophile host-vector systems. Homologous and heterologous expression of the thermophilic proteins which were difficult to produce in full active form from mesophilic hosts was successfully developed in thermophiles, especially in T. thermophilus, and several hyperthermophilic archaea using their host-vector systems, “genetic and metabolic engineering,” were developed for biofuel production in thermophiles, especially in thermophilic ethanologens. And, directed evolution methods were developed for thermo-adaptation of mesophilic proteins in thermophiles such as T. thermophilus.
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References
Aagaard C, Phan H, Trevisanato S, Garrett RA (1994) J Bacteriol 176:7744–7747
Aagaard C, Leviev I, Aravalli RN, Forterre P, Prieur D, Garrette RA (1996) FEMS Microbiol Rev 18:93–104
Albers SV, Driessen AJM (2007) Archaea 2:145–149
Albers SV, Jonuscheit M, Dinkelaker S, Urich T, Kletzin A, Tampé R, Driessen AJM, Schleper C (2006) Appl Environ Microbiol 72:102–111
Allers T, Mevarech M (2005) Nat Rev Genet 6:58–73
Allers T, Barak S, Liddell S, Wardell K, Mevarech M (2010) Appl Environ Microbiol 76:1759–1769
Angelov A, Mientus M, Liebl S, Liebl W (2009) Syst Appl Microbiol 32:177–185
Aravalli RN, Garret RA (1997) Extremophiles 1:183–191
Atomi H, Sato T, Kanai T (2012) Curr Opin Biotechnol 22:618–626
Averhoff B (2004) J Bioenerg Biomembr 36:25–33
Averhoff B (2006) Genetic Systems for Thermus, Gene Transfer Systems for Obligately Anaerobic Thermophilic Bacteria. In: Rainey FA, Oren A (eds) Methods in biotechnology, vol 35. Elsevier Ltd, New York, pp 279–308
Barnard D, Casanueva A, Tuffin M, Cowan D (2010) Environ Technol 31:871–888
Basen M, Sun J, Adams MWW (2012) MBio 3:1–8
Berkner S, Lipps G (2008) Arch Microbiol 190:217–230
Berkner S, Wlodkowski A, Albers SV, Lipps G (2010) Extremophiles 14:249–259
Bini E (2010) FEMS Microbiol Ecol 73:1–16
Bjornsdottir SH, Thorbjarnardottir SH, Eggertsson G (2005) Appl Microbiol Biotechnol 66:675–682
Bjornsdottir SH, Blondal T, Hreggvidsson GO, Eggertsson G, Petursdottir S, Hjorleifsdottir S, Thorbjarnardottrir SH, Kristjansson JK (2006) Extremophiles 10:1–6
Bjornsdottir SH, Fridjjonsson OH, Kristjansson JK, Eggertsson G (2007) Extremophiles 11:283–293
Blas-Galindo E, Cava F, Lopez-Vinas E, Mendieta J, Berenguer J (2007) Appl Environ Microbiol 73:5138–5145
Blondal T, Hjorleifsdottir S, Aevarsson A, Fridjonsson OH, Skirnisdottir S, Weat JO, Hermannsdottir AG, Hreggvidsson GO, Smith AV, Kristjansson JK (2005) J Biol Chem 280:5188–5194
Brouns SJWH, Akerboom J, Turnbull AP, de Vos WM, van der Oost J (2005) J Biol Chem 280:11422–11431
Brügger K, Torarinsson E, Redder P, Chen L, Garrette RA (2004) Biochem Soc Trans 32:179–183
Burdette DS, Secundo F, Phillips RS, Dong J, Scott RA, Zeikus JG (1997) Biochem J 326:717–724
Burdette DS, Jung SH, Shen GJ, Hollingsworth RI, Zeikus JG (2002) Appl Environ Microbiol 68:1914–1918
Cannio R, Contursi P, Rossi M, Bartolucci S (2001) Extremophiles 5:153–159
Cava F, Laptenko O, Borukhov S, Chahlafi Z, Bias-Galindo E, Gomez-Puertas P, Barenguer J (2007) Mol Microbiol 64:630–646
Cava F, de Pedero MA, Blas-Galindo E, Waldo GS, Westblade LF, Berenguer J (2008) Environ Microbiol 10:605–613
Cava F, Hidalgo A, Berenguer J (2009) Extremophiles 13:213–231
Chandrayan SK, McTernan PM, Hopkins RC, Sun J, Jenney FE Jr, Adams MWW (2012) J Biol Chem 287:3257–3264
Charbonnier F, Forterre P (1994) J Bacteriol 176:1251–1259
Chautard H, Blas-Galindo E, Menguy T, Grand’Moursel L, Cava F, Berenguer J, Delcourt M (2007) Nat Methods 4:919–921
Chen CK, Boucle CM, Blaschek HP (1996) FEMS Microbiol Lett 140:185–191
Contursi P, Cannio R, Prato S, Fiorentino G, Rossi M, Bartolucci S (2003) FEMS Microbiol Lett 218:115–120
Contursi P, Pisani FM, Grigoriev A, Cannio R, Bartolucci S, Rossi M (2004) Extremophiles 8:385–391
Cripps RE, Eley K, Leak DJ, Rudd B, Taylor MP, Boakes S, Martin S, Atkinson T (2009) Metab Eng 11:398–408
de Grade M, Lasa I, Berenguer J (1998) FEMS Microbiol Lett 165:51–57
Deng L, Zhu H, Chen Z, Liang YX, She Q (2009) Extremophiles 13:735–746
Desai SG, Guerinot ML, Lynd LR (2004) Appl Microbiol Biotechnol 65:600–605
Dien BS, Cotta MA, Jeffries TW (2003) Appl Microbiol Biotechnol 63:258–266
Erauso G, Prieur D, Godfroy A, Raguénes G (1995) Plate Cultivation Technique for Strictly Anaerobic, Thermophilic, Sulfur-metabolizing Archaea. In: Fleiscmann EM, Place AR, Robb FT, Schreier JJ (eds) Archaea: a laboratory manual, vol 3. Cold Spring Harbpr Laboratory Press, Cold Spring Harbor, pp 25–29
Farkas J, Chung D, DeBarry M, Adams MWW, Westpheling J (2011) Appl Environ Microbiol 77:6343–6349
Fridjonsson O, Watzlawick H, Mattes R (2002) J Bacteriol 184:3385–3391
Garrett RA, Prangishvili D, Shah SA, Reuter M, Stetter KO, Peng X (2010) Environ Microbiol 12:2918–2930
Georgieva T, Mikkelsen MJ, Ahring BK (2007) Cent Eur J Biol 2:364–377
Gorlas A, Koonin EV, Bienvenu N, Prieur D, Geslin C (2012) Environ Microbiol 14:503–516
Grogan DW (1996) J Bacteriol 178:3207–3211
Grogan DW, Stengel KR (2008) Mol Microbiol 69:1255–1265
HasenÖhrl D, Lombo T, Kaberdin V, Londel P, Bläsi U (2008) Proc Natl Acad Sci 105:2146–2150
Henche AL, Koerdt A, Ghosh A, Albers SV (2012) Environ Microbiol 14:779–793
Hidaka Y, Hasegawa M, Nakahara T, Hoshino T (1994) Bisosci Biotechnol Biochem 58:1338–1339
Hidalgo A, Betancor L, Moreno R, Zafra O, Cava F, Fernandez-Lafuente R, Guisan JM, Berenguer J (2004) Appl Environ Microbiol 70:3839–3844
Ishida M, Oshima T, Yutani K (2002) FEMS Microbiol Lett 216:179–183
Jennert KCB, Tardif C, Young DI, Young M (2000) Microbiology 146:3071–3080
Jenney FE Jr, Adams MW (2008) Extremophiles 12:39–50
Jiang Y, Zhou Q, Wu K, Li XQ, Shao WL (2006) FEMS Microbiol Lett 259:254–259
Jonuscheit M, Martusewitsch E, Stedman KM, Schleper C (2003) Mol Microbiol 48:1241–1252
Kayser KJ, Kwak JH, Park HS, Kilbane JJ 2nd (2001) Lett Appl Microbiol 32:412–418
Kiss C, Temirov J, Chasteen L, Waldo GS, Bradbury RM (2009) Protein Eng Des Sel 22:313–323
Klapatch TR, Guerinot ML, Lynd LR (1996) J Ind Microbiol 16:342–347
Koyama Y, Hoshino T, Tomizuka N, Furukawa K (1986) J Bacteriol 166:338–340
Koyama Y, Okamoto S, Furukawa K (1990) Appl Environ Microbiol 56:2251–2254
Kurosawa N, Grogan DW (2005) FEMS Microbiol Lett 253:141–149
Laptenko O, Kim SS, Lee J, Starodubtseva M, Cava F, Berenguer X, Kong P, Borukhov S (2007) EMBO J 25:2131–2141
Lasa I, de Grado M, de Pedro MA, Berenguer J (1992) J Bacteriol 174:6424–6431
Liao H, Mckenzie T, Hageman R (1986) Proc Natl Acad Sci 83:576–580
Lipps G (2006) Extremophiles 10:17–28
Lipps G (2007) In: Garrett R, Klenl HP (eds) Archaea: evolution, physiology, and molecular biology. Blackwell Publishing Ltd, Malden pp 105–112
Lipscomb GL, Stirrett K, Schut GJ, Yang F, Jenney FE Jr, Scott RA, Adams MWW, Westpheling J (2011) Appl Environ Microbiol 77:2232–2238
Liu B, Zhang X (2008) Appl Microbiol Biotechnol 80:697–707
Lucas S, Toffin L, Zivanovic Y, Charlier D, Moussard H, Forterre P, Prieur D, Erauso G (2002) Appl Environ Microbiol 68:5528–5536
Mai V, Wiegel J (2000) Appl Environ Microbiol 66:4817–4821
Mai V, Lorenz WW, Wiegel J (1997) FEMS Microbiol Lett 148:163–167
Martusewitsch E, Sensen CW, Schleper C (2000) J Bacteriol 182:2574–2581
Maseda H, Hoshino T (1995) FEMS Microbiol Lett 128:127–134
Matsumi R, Manabe K, Fukui T, Atomi H, Imanaka T (2007) J Bacteriol 189:2683–2691
Matsumura M, Aiba S (1985) J Biol Chem 260:15298–15303
Meile L, Abendschein P, Leisinger T (1990) J Bacteriol 172:3507–3508
Mevarech M, Allers T (2007) In: Garrett R, Klenl HP (eds) Archaea: evolution, physiology, and molecular biology. Blackwell Publishing Ltd, Malden pp 125–136
Minakhin L, Goel M, Berdygulova Z, Ramanculov E, Florens L, Glazko G, Karamychev VN, Slesarev AI, Kozyavkin SA, Khromov I, Ackermann HW, Washburn M, Mushegian A, Severinov K (2008) J Mol Biol 378:468–480
Mochizuki T, Yoshida T, Tanaka R, Forterre P, Sako Y, Prangishvili D (2010) Virology 402:347–352
Mochizuki T, Sako Y, Prangishvili D (2011) J Bacetriol 193:5412–5419
Moreno R, Zafra O, Cava F, Berenguer J (2003) Plasmid 49:2–8
Moreno R, Haro A, Castellanos A, Berenguer J (2005) Appl Environ Microbiol 71:591–593
Mueller M, Takemasa R, Schwarz A, Atomi H, Imanaka T, Reeve JN (2009) Biochim Biophys Acta 1794:1709–1714
Nakamura A, Takakura Y, Kobayashi H, Hoshino T (2005) J Biosci Bioeng 100:158–163
Narumi I, Sawakami K, Nakamoto S, Nakayama N, Yanagisawa T, Takahashi N, Kihara H (1992) Biotechnol Tech 6:83–86
Park HS, Kayser KJ, Kwak JH, Kilbane JJ 2nd (2004) J Ind Microbiol Biotechnol 31:189–197
Pedelacq JD, Cabantous S, Tran T, Terwillinger TC, Waldo GS (2006) Nat Biotechnol 24:79–88
Peng H, Fu B, Mao Z, Shao W (2006) Biotechnol Lett 28:1913–1917
Pina M, Bize A, Forterre P, Prangishvili D (2011) FEMS Microbiol Rev 35:1035–1054
Prangishvili D, Forterre P, Garret RA (2006) Nat Rev Microbiol 4:837–848
Ramírez-Acros S, Moreno R, Zafra O, Castan P, Valles C, Berenguer J (1998) J Bacteriol 180:3137–3143
Redder P, Garrett RA (2006) J Bacteriol 188:4198–4206
Redder P, Peng X, Brügger K, Shah SA, Roesch F, Greve B, She Q, Schleper C, Forterre P, Garrett RA, Prangishvili D (2009) Environ Microbiol 11:2849–2862
Rhee MS, Kim JW, Qian Y, Ingram LO, Shanmugam KT (2007) Plasmid 58:13–22
Santangelo TJ, Čuboňoava L, Reeve JN (2008) Appl Environ Microbiol 74:3099–3104
Santangelo TJ, Cuboňoava L, Skinner KM, Reeve JN (2009) J Bacteriol 191:7102–7108
Santangelo TJ, Čuboňoava L, Reeve JN (2010) Appl Environ Microbiol 76:1044–1052
Santangelo TJ, Čuboňoava L, Reeve JN (2011) Mol Microbiol 81:897–911
Sato T, Fukui T, Atomi H, Imanaka T (2003) J Bacteriol 185:210–220
Sato T, Fukui T, Atomi H, Imanaka T (2005) Appl Environ Microbiol 71:3889–3899
Schelert J, Dixit V, Hoang V, Simbahan J, Drozda M, Blum P (2004) J Bacteriol 186:427–437
Schleper C, Roder R, Singer T, Zilling W (1994) Mol Gen Genet 243:91–96
Schleper C, Holz I, Janekovic D, Murphy J, Zilling W (1995) J Bacteriol 177:4417–4426
Schreier HJ, Robinson-Bidle KA, Romashko AM, Patel GV (1999) Extremophiles 3:11–19
Shaw AJ, Podkaminer KK, Desai SG, Bardsley JS, Rogers SR, Thorne PG, Hogsett DA, Lynd LR (2008) Proc Natl Acad Sci USA 105:13769–13774
Shaw AJ, Hosgett DA, Lynd LR (2010) Appl Environ Microbiol 76:4713–4719
She Q, Singh RK, Confalonieri F, Zivanovic Y, Allard G, Awayez MJ, Chan-Weiher CC, Clausen IG, Curtis BA, De Moors A, Erauso G, Fletcher C, Gordon PM, Heikamp-de Jong I, Jeffries AC, Kozera CJ, Medina N, Peng X, Thi-Ngoc HP, Redder P, Schenk ME, Theriault C, Tolstrup N, Charlebois RL, Doolittle WF, Duguet M, Gaasterland T, Garret RA, Sensen CW, Van der Oost J (2001) Proc Natl Acad Sci 98:7835–7840
She Q, Zhang C, Deng L, Peng N, Chen Z, Liang YX (2009) Biochem Soc Trans 37:92–96
Soler N, Marguet E, Cortez D, Desnoues N, Keller J, Tilbeurgh HV, Sezonov G, Forterre P (2010) Nucleic Acids Res 28:1–17
Soutscheck-Bauer E, Hartl L, Staudenbauer WL (1986) Biotecnol Lett 7:705–710
Tailliez P, Girard H, Millet J, Beguin P (1989a) Appl Environ Microbiol 55:207–211
Tailliez P, Girard H, Longin R, Beguin P, Millet J (1989b) Appl Environ Microbiol 55:203–206
Takemasa R, Yokooji Y, Yamatsu A, Atomi H, Imanaka T (2011) Appl Environ Microbiol 77:2392–2398
Tamakoshi M, Yamagishi A, Oshima T (1995) Mol Microbiol 16:1031–1036
Tamakoshi M, Yaoi T, Oshima T, Yamagishi A (1999) FEMS Microbiol Lett 173:431–437
Tamakoshi M, Nakano Y, Kakizawa S, Yamagishi A, Oshima T (2001) Extremophiles 5:17–22
Taylor MP, Esteban CD, Leak DJ (2008) Plasmid 60:45–52
Taylor MP, Eley KL, Martin S, Tuffin MI, Burton SG, Cowan DA (2009) Trends Biotechnol 27:398–405
Thompson AH, Studholme DJ, Green EM, Leak DJ (2008) Biotechnol Lett 30:1359–1365
Tuffin M, Anderson D, Heath C, Cowan D (2009) Biotechnol J 4:1671–1683
Tyurin MV, Deseai SG, Lynd LR (2004) Appl Environ Microbiol 70:883–890
Tyurin MV, Sullivan CR, Lynd LR (2005) Appl Environ Microbiol 71:8069–8076
Tyurin MV, Lynd LR, Wiegel J (2006) Genetic Systems for Thermus, Gene Transfer Systems for Obligately Anaerobic Thermophilic Bacteria. In: Rainey FA, Oren A (eds) Methods in biotechnology, vol 35. Elsevier Ltd, New York, pp 309–330
van der Oost J, Jore MM, Weatra ER, Lundgren M, Brouns SJ (2009) Trends Biochem Sci 34:401–407
van Kovács AT, Hartskamp M, Kuipers OP, van Kranenburg R (2010) Appl Environ Microbiol 76:4085–4088
van Lynd LR, Zyl WH, McBride JE, Laser M (2005) Curr Opin Biotechnol 16:577–583
Vee Aune TE, Aachmann FL (2010) Appl Microbiol Biotechnol 85:1301–1313
Waege I, Schmid G, Thumann S, Thomm M, Hausner W (2010) Appl Environ Microbiol 76:3308–3313
Wartrin L, Martin-Jezequel V, Prieur D (1995) Appl Environ Microbiol 61:1138–1140
Wei D, Zhang X (2010) J Virol 84:2365–2373
Worrell VE, Nagle DP Jr, McCarthy D, Eisenbraun A (1988) J Bacteriol 170:653–656
Worthington P, Hoang V, Perez-Pomares F, Blum P (2003) J Bacteriol 185:482–488
Wu L, Welker NE (1989) J Gen Microbiol 135:1315–1324
Yao S, Mikkelsen MJ (2010) Appl Microbiol Biotechnol 88:199–208
Yokooji Y, Tomita H, Atomi H, Imanaka T (2009) Biochim Biophys Acta 284:28137–28145
Yu JS, Vargas M, Mityas C, Noll KM (2001) Extremophiles 5:53–60
Yu MX, Slater MR, Ackermann HW (2006) Arch Environ 151:663–679
Zaldivar J, Nielson J, Olsson L (2001) Appl Microbiol Biotechnol 56:17–34
Zaparty M, Esser D, Gertig S, Haferkamp P, Kouril T, Manica A, Pham TK, Reimann J, Schreiber K, Sierocinski P, Teichmann D, van Wolferen M, von Jan M, Wieloch P, Albers SV, Driessen AJM, Klenk HP, Schleper C, Schomburg D, van der Oost J, Wright PC, Siebers B (2010) Extremophiles 14:119–142
Zhang C, Whitaker RJ (2012) Microbiology 158:1513–1522
Zheng T, Huang Q, Zhang C, Ni J, She Q, Shen Y (2012) Appl Environ Microbiol 78:568–574
Zyprian E, Matzura H (1986) DNA 5:219–225
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Inoue, T., Sako, Y. (2013). Host-Vector Systems in Thermophiles. In: Satyanarayana, T., Littlechild, J., Kawarabayasi, Y. (eds) Thermophilic Microbes in Environmental and Industrial Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5899-5_13
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