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Stromule Formation

  • John C. GrayEmail author
Chapter
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 36)

Summary

Stromules are highly dynamic stroma-filled tubules extending from the surface of plastids in all multicellular plants. Although stromules may interconnect two, or more, plastids and allow the transfer of stromal proteins as large as Rubisco (∼550 kDa) between plastids, their function is still largely a matter of conjecture. They may increase the plastid surface area to facilitate movement of materials into or out of plastids, be involved in sensing the cellular environment, and/or have signaling functions due to close apposition of stromules and nuclei, plasma membrane and other cell organelles. Stromule formation appears unrelated to chloroplast division or to light-intensity-dependent chloroplast movement. Stromules are most easily observed by confocal microscopy of cells expressing plastid-targeted fluorescent proteins, and the definition of stromules is based on such observations. Identification of stromules in electron microscope images is problematic, and ideally requires examination of thin serial sections. In leaves of both monocots and dicots, stromules are most abundant in epidermal cell-types, such as trichomes, guard cells and pavement cells, and are more difficult to observe in mesophyll cells containing large closely packed chloroplasts. Stromule formation and movement depends on the actin cytoskeleton and requires the ATPase activity of myosin XI proteins. A 42-amino-acid-residue region of myosin XI that directs myosin XI to the chloroplast periphery offers prospects for identification of stromule components required for stromule mobility. Stromules are affected by various environmental factors and by biotic stresses. Stromules are induced by water stress, acting via abscisic acid signaling pathways, and by viral infection. Stromule abundance is also affected by light and temperature.

Keywords

Green Fluorescent Protein Guard Cell Mesophyll Cell Bundle Sheath Subsidiary Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations:

ABA

Abscisic acid;

ACC

1-amino­cyclopropane-1-carboxylic acid;

APM

Amiprophosmethyl;

BDM

2,3-butanedione 2-monoxime;

BiFC

Bimolecular fluorescence complementation;

CFP

Cyan fluorescent protein;

DIC

Differential interference contrast;

GFP

Green fluorescent protein;

OEP14

Outer envelope protein of 14 kDa;

PEG

Polyethylene glycol;

TIC

Translocon of the inner chloroplast envelope membrane;

TMV

Tobacco mosaic virus;

TOC

Translocon of the outer chloroplast envelope membrane;

TPT

Triose phosphate–phosphate translocator;

VIGS

Virus-induced gene silencing;

YFP

Yellow fluorescent protein

Notes

Acknowledgments

I am extremely grateful to Jim Sullivan, Michael Hansen and Senthil Natesan for providing previously unpublished images of stromules, and to John Carr for discussion of virus infection. The work in my laboratory on stromules has been supported by research grants from the Biotechnology and Biological Sciences Research Council (BBSRC).

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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Plant SciencesUniversity of CambridgeCambridgeUK

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