Abstract
Genomics tools are revolutionizing the way we study developmental processes. Microarrays and RNA-Seq allow the sensitive, quantitative and global analysis of gene expression patterns. Instead of examining a few select genes, it is now routine to generate global gene expression profiles of developing systems. The early experiments examined entire developing organs, thereby identifying, for example, all transcription factors, growth factors, and receptors expressed. Subsequent experiments dramatically improved the genomics analysis by providing higher spatio-temporal resolution. Organ development is remarkably complex, with discrete substructures, a developmental time course of events, and the simultaneous differentiation of discrete cell types. It is not possible, therefore, to homogenize the developing organ, apply genomics tools, and derive deep insight. Laser capture microdissection allows the purification of single developmental compartments. Transgenic mice with gene specific promoters driving the expression of markers, such as green fluorescent protein, can be used to purify specific cell types from developing organs. Even higher resolution analysis can be carried out with tools that allow the gene expression profiling of single cells. The use of these genomics approaches produces enormous volumes of data that need to be captured, annotated in a systematic way, stored, integrated and analyzed, using the resources of biomedical informatics.
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This work was supported in part by NIH grant number RC4-DK090891.
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Brunskill, E.W., Potter, A.S., Potter, S.S. (2012). Defining Genetic Blueprints – Kidney and Craniofacial Development. In: Hutton, J. (eds) Pediatric Biomedical Informatics. Translational Bioinformatics, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5149-1_18
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DOI: https://doi.org/10.1007/978-94-007-5149-1_18
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