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Metabolic Engineering of Microorganisms for Vitamin C Production

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Reprogramming Microbial Metabolic Pathways

Part of the book series: Subcellular Biochemistry ((SCBI,volume 64))

Abstract

Vitamin C, an important organic acid, is widely used in the industries of pharmaceuticals, cosmetics, food, beverage and feed additives. Compared with the Reichstein method, biotechnological production of vitamin C is an attractive approach due to the low cost and high product quality. In this chapter, biosynthesis of vitamin C, including one-step fermentation processes and two-step fermentation processes are discussed and compared. Furthermore, the prospects of the biotechnological production of vitamin C are also presented.

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Abbreviations

AA-2G:

2-O-alpha-D-glucopyranosyl-L-ascorbic acid

2,5-DKG:

2,5-diketo-D-gluconate

2,5-DKGR:

2,5-diketo-D-gluconic acid reductase

GUL oxidase:

L-gulono-1,4-lactone oxidase

2-KLG:

2-keto-L-gulonic acid

2-KLGR:

2-keto-L-gulonic acid reductase

ORFs:

open reading frames

PQQ:

pyrro-quinoline quinone

SDH:

L-sorbose dehydrogenase

SLDH:

D-sorbitol dehydrogenase

SNDH:

L-sorbosone dehydrogenase

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Jingwen Zhou, Guocheng Du & Jian Chen

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  1. Jingwen Zhou
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  2. Guocheng Du
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  3. Jian Chen
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Correspondence to Jian Chen .

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Editors and Affiliations

  1. , State Key Laboratory, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, Jiangsu, China, People's Republic

    Xiaoyuan Wang

  2. School of Biotechnology, Jiangnan University, Lihu Avenue 1800, Wuxi, 214122, China, People's Republic

    Jian Chen

  3. , Div. of Life Sciences, King's College London, Stamford Street 150, London, SE1 9NH, United Kingdom

    Peter Quinn

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Zhou, J., Du, G., Chen, J. (2012). Metabolic Engineering of Microorganisms for Vitamin C Production. In: Wang, X., Chen, J., Quinn, P. (eds) Reprogramming Microbial Metabolic Pathways. Subcellular Biochemistry, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5055-5_12

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