Cell-Free Protein Synthesis Using E. coli Cell Extract for NMR Studies

  • Mitsuhiro Takeda
  • Masatsune KainoshoEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 992)


The use of cell-free protein production systems for producing isotope labeled proteins generates new opportunities to perform unprecedented NMR studies. As compared with conventional cellular expression systems, the scrambling and dilution of amino acids are highly suppressed in the cell-free reaction, allowing the production of proteins with a wide variety of residue and site-specific isotope labeling patterns. In this chapter, the procedure for cell-free protein synthesis for NMR studies, using an E. coli extract, is introduced.


Dialysis Solution Label Amino Acid Protein Synthesis Machinery Endogenous Amino Acid Isotope Label Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Structural Biology Research Center, Graduate School of ScienceNagoya UniversityNagoyaJapan
  2. 2.Center for Priority Areas, Graduate School of Science and TechnologyTokyo Metropolitan UniversityHachiojiJapan

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